LOCUS V045013 6843 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V045013 VERSION V045013 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 955..2358 /label="Ppara(NM_011144)" /note="Ppara(NM_011144)" /gene="Ppara" CDS 2380..2409 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" polyA_signal 2438..2662 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2708..3136 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3150..3479 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3546..4340 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4516..4637 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4686..4702) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4710..4726 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4734..4764) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4779..4800 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5088..5676) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5847..6707) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6708..6812) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattcgc caccatggtg 961 gacacagaga gccccatctg tcctctctcc ccactggagg cagatgacct ggaaagtccc 1021 ttatctgaag aattcttaca agaaatggga aacattcaag agatttctca gtccatcggt 1081 gaggagagct ctggaagctt tggttttgca gactaccagt acttaggaag ctgtccgggc 1141 tccgagggct ctgtcatcac agacaccctc tctccagctt ccagcccttc ctcagtcagc 1201 tgccccgtga tccccgccag cacggacgag tcccccggca gtgccctgaa catcgagtgt 1261 cgaatatgtg gggacaaggc ctcagggtac cactacggag ttcacgcatg tgaaggctgt 1321 aagggcttct ttcggcgaac tattcggctg aagctggtgt acgacaagtg tgatcggagc 1381 tgcaagattc agaagaagaa ccggaacaaa tgccagtact gccgttttca caagtgcctg 1441 tctgtcggga tgtcacacaa tgcaattcgc tttggaagaa tgccaagatc tgaaaaagca 1501 aaactgaaag cagaaattct tacctgtgaa cacgacctga aagattcgga aactgcagac 1561 ctcaaatctc tgggcaagag aatccacgaa gcctacctga agaacttcaa catgaacaag 1621 gtcaaggccc gggtcatact cgcgggaaag accagcaaca acccgccttt tgtcatacat 1681 gacatggaga ccttgtgtat ggccgagaag acgcttgtgg ccaagatggt ggccaacggc 1741 gtcgaagaca aagaggcaga ggtccgattc ttccactgct gccagtgcat gtccgtggag 1801 accgtcacgg agctcacaga atttgccaag gctatcccag gctttgcaaa cttggacttg 1861 aacgaccaag tcaccttgct aaagtacggt gtgtatgaag ccatcttcac gatgctgtcc 1921 tccttgatga acaaagacgg gatgctgatc gcgtacggca atggctttat cacacgcgag 1981 ttccttaaga acctgaggaa gccgttctgt gacatcatgg aacccaagtt tgacttcgct 2041 atgaagttca atgccttaga actggatgac agtgacattt ccctgtttgt ggctgctata 2101 atttgctgtg gagatcggcc tggccttcta aacataggct acattgagaa gttgcaggag 2161 gggattgtgc acgtgcttaa gctccacctg cagagcaacc atccagatga caccttcctc 2221 ttcccaaagc tccttcaaaa aatggtggac cttcggcagc tggtcacgga gcatgcgcag 2281 ctcgtacagg tcatcaagaa gaccgagtcc gacgcagcgc tgcacccact gttgcaagag 2341 atctacagag acatgtacct cgagtctaga gggcccttcg aacaaaaact catctcagaa 2401 gaggatctgt gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca 2461 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc 2521 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg 2581 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct 2641 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg 2701 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 2761 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 2821 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 2881 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 2941 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt 3001 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 3061 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 3121 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 3181 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 3241 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 3301 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 3361 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg 3421 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 3481 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt 3541 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc 3601 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc 3661 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg 3721 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag 3781 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg 3841 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg 3901 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac 3961 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg 4021 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc 4081 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg 4141 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc 4201 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc 4261 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc 4321 ttcttgacga gttcttctga gcgggactct ggggttcgcg aaatgaccga ccaagcgacg 4381 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 4441 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 4501 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc 4561 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 4621 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa 4681 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 4741 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 4801 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 4861 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 4921 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 4981 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 5041 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 5101 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 5161 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 5221 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 5281 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 5341 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 5401 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 5461 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 5521 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 5581 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 5641 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 5701 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 5761 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 5821 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 5881 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 5941 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 6001 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 6061 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 6121 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 6181 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 6241 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 6301 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 6361 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 6421 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 6481 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 6541 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 6601 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 6661 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 6721 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 6781 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 6841 gtc //