LOCUS       V022763                 8240 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022763
VERSION     V022763
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             902..3679
                     /label="PTPN4(NM_002830)"
                     /note="PTPN4(NM_002830)"
                     /gene="PTPN4"
     CDS             3686..3712
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3716..3742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3746..3772
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3807..4031
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4077..4505
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4519..4848
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4915..5709
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5883..6004
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6053..6069)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6077..6093
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6101..6131)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6146..6167
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6455..7040)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7211..8071)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8072..8176)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcgccac
      901 catgacctca cgtttccgat tgcctgctgg cagaacctac aatgtacgag catcagagtt
      961 ggcccgagac agacagcata ctgaagtggt ttgcaacatc cttcttctgg ataacactgt
     1021 acaagctttc aaagtcaata aacatgatca ggggcaagtc ttgttggatg tcgtcttcaa
     1081 gcatctagat ttgactgagc aggactattt tggtttacag ttggctgatg attccacaga
     1141 taacccaagg tggctggatc caaacaaacc aataaggaag cagctaaaga gaggatctcc
     1201 ttacagtttg aactttagag tcaaattttt tgtaagtgac cccaacaagt tacaagaaga
     1261 atatacaagg taccagtatt ttttgcaaat taaacaagac attcttactg gaagattacc
     1321 ctgtccttct aatactgctg cccttttagc ttcatttgct gttcagtctg aacttggaga
     1381 ctacgatcag tcagagaact tgtcaggcta cctctcagat tattctttca ttcctaatca
     1441 acctcaagat tttgaaaaag aaattgcaaa attacatcag caacacatag gcttatctcc
     1501 tgcagaagca gaatttaatt acctaaacac agcacgtacc ttagaactct atggagttga
     1561 attccactat gcaagggatc agagtaacaa tgaaattatg attggagtga tgtcaggagg
     1621 aattctgatt tataagaaca gggtacgaat gaataccttt ccatggttga agattgtaaa
     1681 aatttctttt aagtgcaaac agttttttat tcaacttaga aaagaattgc atgaatctag
     1741 agaaacatta ttgggattta atatggtgaa ttacagagca tgtaaaaatt tgtggaaagc
     1801 atgtgtagaa catcacacat tcttccgttt ggacagacca cttccacctc aaaagaattt
     1861 ttttgcacat tattttacat taggttcaaa attccggtac tgtgggagaa ctgaagtcca
     1921 atcagttcag tatggcaaag aaaaggcaaa taaagacagg gtatttgcaa gatccccaag
     1981 taagcccttg gcacggaaat taatggattg ggaagtagta agcagaaatt caatatctga
     2041 tgacaggtta gaaacacaaa gtcttccatc acgatctcca ccgggaactc ctaatcatcg
     2101 aaattctaca ttcacgcagg aaggaacccg gttacgacca tcttcagttg gtcatttggt
     2161 agaccatatg gttcatactt ccccaagcga agtgtttgta aatcagagat ctccgtcatc
     2221 aacacaagct aatagcattg ttctggaatc atcaccatca caagagaccc ctggagatgg
     2281 gaagcctcca gctttaccac ccaaacagtc aaagaaaaac agttggaacc aaattcatta
     2341 ttcacattcg caacaagatc tagaaagtca tattaatgaa acatttgata ttccatcttc
     2401 tcctgaaaaa cccactccta atggtggtat tccacatgat aatcttgtcc taatcagaat
     2461 gaaacctgat gaaaatggga ggtttggatt caatgtaaag ggaggatatg atcagaagat
     2521 gcctgtgatt gtgtctcgag tagcaccagg aacacctgct gacctctgtg tccctagact
     2581 gaatgaaggg gaccaagttg tactgatcaa tggtcgggac attgcagaac acactcatga
     2641 tcaggttgtg ctgtttatta aagctagttg tgagagacat tctggggaac tcatgcttct
     2701 agttcgacct aatgctgtat atgatgtagt ggaagaaaag ctagaaaatg agccagattt
     2761 ccagtatatt cctgagaaag ccccactaga tagtgtgcat caggatgacc attccctgcg
     2821 ggagtcaatg atccagctag ctgaggggct tatcactgga acagtcctga cacagtttga
     2881 tcaactgtat cggaaaaaac ctggaatgac aatgtcctgt gccaaattac ctcagaatat
     2941 ttccaaaaat agatacagag atatttcgcc ttatgatgcc acacgggtca ttttaaaagg
     3001 taatgaagac tacatcaatg cgaactatat aaatatggaa attccttctt ccagcattat
     3061 aaatcagtac attgcttgtc aagggccatt accacacact tgtacagatt tttggcagat
     3121 gacttgggaa caaggctcct ctatggttgt aatgttgacc acacaagttg aacgtggcag
     3181 agttaaatgt caccaatatt ggccagaacc cacaggcagt tcatcttatg gatgctacca
     3241 agttacctgc cactctgaag aaggaaacac tgcctatatc ttcaggaaga tgaccctatt
     3301 taaccaagag aaaaatgaaa gtcgtccact cactcagatc cagtacatag cctggcctga
     3361 ccatggagtc cctgatgatt cgagtgactt tctagatttt gtttgtcatg tacgaaacaa
     3421 gagggctggc aaggaagaac ccgttgttgt ccattgcagt gctggaatcg gaagaactgg
     3481 ggttcttatt actatggaaa cagccatgtg tctcattgaa tgcaatcagc cagtttatcc
     3541 actagatatt gtaagaacaa tgagagatca gcgagccatg atgatccaaa cacctagtca
     3601 atacagattt gtatgtgaag ctattttgaa agtttatgaa gaaggctttg ttaaaccctt
     3661 aacaacatca acaaataaac tcgagtaccc atacgacgta cctgattatg caacttatcc
     3721 ttatgatgta cctgattatg ctagctatcc ttatgatgtc ccagactacg cataagggcc
     3781 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     3841 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     3901 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     3961 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     4021 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     4081 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4141 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4201 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4261 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4321 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4381 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4441 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4501 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     4561 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4621 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4681 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4741 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     4801 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     4861 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     4921 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     4981 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     5041 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     5101 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     5161 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     5221 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     5281 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     5341 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     5401 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     5461 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     5521 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     5581 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     5641 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     5701 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     5761 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     5821 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     5881 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5941 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6001 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     6061 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6121 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6181 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6241 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6301 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6361 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6421 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6481 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6541 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6601 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6661 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6721 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6781 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6841 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6901 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6961 gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc
     7021 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7081 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7141 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7201 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7261 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7321 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7381 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7441 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7501 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7561 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7621 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7681 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7741 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7801 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7861 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7921 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7981 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8041 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8101 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8161 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     8221 atctcccgat cccctatggt
//
