LOCUS       V023498                 9023 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023498
VERSION     V023498
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             902..4462
                     /label="PTPN14(NM_005401)"
                     /note="PTPN14(NM_005401)"
                     /gene="PTPN14"
     CDS             4469..4495
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4499..4525
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4529..4555
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    4590..4814
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4860..5288
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5302..5631
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5698..6492
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6666..6787
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6836..6852)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6860..6876
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6884..6914)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6929..6950
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7238..7823)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7994..8854)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8855..8959)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcgccac
      901 catgcctttt ggtctgaagc tccgccggac acggcgctac aacgtcctga gcaagaactg
      961 ctttgtcaca cggattcgcc tgctggacag caatgttatc gagtgcacgc tgtcggtgga
     1021 aagcacaggg caagaatgcc tggaggctgt ggcccagagg ctggagctgc gagagacgca
     1081 ctactttggc ctttggtttc tcagcaagag ccagcaagca cgatgggtgg agctggagaa
     1141 acctctgaag aaacatctgg acaaattcgc taatgagcct ttgcttttct ttggagtcat
     1201 gttctatgtg ccaaatgtgt catggcttca gcaagaggcc acaagatatc agtattacct
     1261 gcaagtcaaa aaagatgtgc ttgaagggcg attacgatgt acattggacc aggtgattcg
     1321 gctagccggc ctagctgtgc aagctgattt tggagactat aatcagtttg attctcaaga
     1381 tttcctcaga gagtatgtgc tatttcctat ggatttggcc ctggaagagg ctgttctgga
     1441 ggagctgacc cagaaggtag cccaagaaca caaagcccac agtggaatcc tgccagcaga
     1501 agctgaactg atgtacatca atgaagttga acgtttggat ggatttggac aggaaatctt
     1561 ccctgtaaag gacaatcatg gaaactgtgt acaccttggc attttcttta tggggatttt
     1621 cgtgaggaac agaattggaa gacaagcggt aatatacagg tggaatgaca tggggaatat
     1681 cactcataac aagtcgacca ttctagtgga gctcatcaac aaagaagaga ctgccctctt
     1741 tcacacggat gatatcgaaa atgccaagta tatttctcgg ttgtttgcca cacgacacaa
     1801 gttttacaaa caaaacaaaa tctgcactga acagtcaaat tctccacccc ccatcagacg
     1861 ccagcccacc tggagccgat cctctctgcc caggcagcag ccgtacatcc tgcctcccgt
     1921 tcacgtccag tgtggtgagc actactcgga aacgcacacc tcgcaagaca gcatttttca
     1981 tgggaatgaa gaagccttgt attgcaactc tcacaacagc ctggacttaa attatttaaa
     2041 tggcactgtc accaatggca gcgtgtgtag cgttcacagc gtcaactccc tcaactgctc
     2101 gcaaagtttc atccaggcct cccctgtatc ctccaacctc agtatccctg ggagtgacat
     2161 catgcgggcc gactacatcc cgagccaccg gcacagcgcg atcatcgtgc cctcgtacag
     2221 gccaaccccc gattatgaga cagtcatgcg ccagatgaag agggggatcc tgcatacaga
     2281 cagccagagc cagtctctga gaaacctcaa cattatcaac acccatgcct acaaccagcc
     2341 agaggatctg gtgtacagcc aaccggagat gcgggagagg cacccctaca ctgtccctta
     2401 tgggccacag ggggtctaca gcaacaaact tgtcagtcca tctgaccaga ggaacccaaa
     2461 gaataatgtg gtaccaagca agccgggggc aagcgccatc tcgcacacgg tgagcacccc
     2521 agagctggcc aacatgcagc tgcagggcag ccataactac agcacggccc acatgcttaa
     2581 gaactatctc ttcaggccac cgccccccta cccacggcca cgacctgcca ccagcacccc
     2641 agacctggcc agccaccgcc acaagtacgt cagcggcagc agcccggacc tggtgacccg
     2701 gaaggtgcag ctctcggtga agaccttcca agaggacagc tctccggtgg ttcatcagtc
     2761 tctccaggag gtgagcgagc ccctcacggc caccaagcac cacggcactg tgaacaagcg
     2821 ccacagcctg gaggtgatga acagcatggt gcggggcatg gaggccatga cgctcaagtc
     2881 gctccacctc cccatggctc gccgcaacac gctccgggag cagggaccgc ccgaggaggg
     2941 gtcaggcagc cacgaggtcc cccagctccc tcagtatcac cacaagaaga ccttctctga
     3001 tgccactatg ctaatccaca gcagcgagag tgaggaggag gaggaggagg ctccagaatc
     3061 ggtgccccag atccccatgc tccgggagaa gatggagtac agtgcccagc tgcaggcggc
     3121 cctggcccgc atccccaaca agcccccgcc tgagtacccc ggtccaagga agagtgtgag
     3181 caatggggct ctgaggcagg accaagccag ccttcctccc gccatggcca gagccagggt
     3241 gctgaggcat gggccagcca aggccatcag catgtctcgg actgacccgc cggctgtcaa
     3301 cggggcctct ctcggcccat ccatctcgga acccgacctg actagtgtga aggagcgggt
     3361 caaaaaagag cctgtgaagg agagacctgt gtctgaaatg ttttccctgg aagacagcat
     3421 tatagagaga gagatgatga tcaggaatct agagaagcag aagatggcag gcctggaggc
     3481 acagaagagg ccgctgatgt tggcagcatt gaatgggctc tcggtggctc gagtctcagg
     3541 gcgggaagag aatcgagttg atgccacccg ggttcccatg gacgagaggt tcagaaccct
     3601 gaagaagaaa ctagaagagg gaatggtgtt cacagaatat gagcaaattc caaagaaaaa
     3661 ggcgaatggc attttcagca cagcagctct gccagaaaac gccgagcgca gccgaatccg
     3721 tgaagttgtc ccctatgagg agaatcgagt agagctgata ccaaccaaag aaaataacac
     3781 aggatacatt aatgcctccc acatcaaggt ggtggttggc ggggcagaat ggcactacat
     3841 agccacccag gggcccctgc cacacacgtg ccacgacttc tggcagatgg tgtgggagca
     3901 gggagtgaat gtgattgcca tggtcactgc agaggaggag ggtggacgaa ccaaaagcca
     3961 ccgatactgg cccaaactag gttcaaagca cagctcagcc acctatggca agttcaaggt
     4021 caccacgaag tttcgaacgg attctgtttg ctatgcaacc acgggcttga aggtcaagca
     4081 ccttttgtct gggcaagaaa ggacggtgtg gcatttacaa tatactgact ggccagatca
     4141 cggctgtcca gaagatgtcc aaggattttt atcctacttg gaggagatcc agtcggtccg
     4201 tcgccatacc aacagcatgc tggaaggcac caagaaccgg cacccgccca tcgtggtcca
     4261 ctgtagtgct ggggtgggaa ggaccggcgt gctcattctt tctgagctga tgatctactg
     4321 cttggaacat aacgaaaagg tggaagtgcc catgatgctg aggctcctca gggagcagag
     4381 gatgttcatg atccagacta tcgctcagta caagtttgtc taccaagtcc tcatccagtt
     4441 cctccaaaac tccagactca ttctcgagta cccatacgac gtacctgatt atgcaactta
     4501 tccttatgat gtacctgatt atgctagcta tccttatgat gtcccagact acgcataagg
     4561 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4621 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4681 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4741 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4801 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     4861 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4921 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4981 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     5041 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     5101 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     5161 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     5221 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     5281 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     5341 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     5401 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     5461 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5521 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     5581 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     5641 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     5701 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     5761 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     5821 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     5881 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     5941 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     6001 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     6061 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     6121 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     6181 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     6241 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     6301 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     6361 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     6421 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     6481 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     6541 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     6601 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     6661 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6721 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6781 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     6841 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6901 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6961 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7021 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7081 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7141 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7201 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7261 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7321 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7381 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7441 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7501 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7561 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7621 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7681 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7741 cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta
     7801 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7861 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7921 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7981 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8041 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8101 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8161 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8221 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8281 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8341 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8401 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8461 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8521 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8581 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8641 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8701 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8761 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8821 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8881 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8941 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     9001 gagatctccc gatcccctat ggt
//
