LOCUS V027982 6676 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V027982 VERSION V027982 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 931..2112 /label="PRMT8(NM_019854)" /note="PRMT8(NM_019854)" /gene="PRMT8" CDS 2119..2208 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" polyA_signal 2243..2467 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2513..2941 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2955..3284 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3351..4145 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4319..4440 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4489..4505) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4513..4529 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4537..4567) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4582..4603 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4891..5476) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5647..6507) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6508..6612) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cactagtcca gtgtggtgga attcgccacc atgggcatga aacactcctc ccgctgcctg 961 ctcctgagga ggaaaatggc ggagaacgcg gccgagagca ccgaggtgaa cagccccccc 1021 tcccagcccc cccagcccgt cgtccctgct aagcccgtgc aatgcgtcca tcatgtgtcc 1081 actcaaccca gctgcccagg acggggcaag atgtccaagc tgctgaaccc agaggagatg 1141 acctcgagag attattactt cgactcctat gcccactttg ggatccacga ggaaatgctg 1201 aaggatgagg tgcggactct cacttaccgg aactccatgt accacaacaa gcacgtgttc 1261 aaggacaaag tggtactgga tgtggggagt ggtactggga tcctttccat gttcgctgcc 1321 aaggcagggg ccaagaaggt gtttgggatc gaatgctcca gtatttctga ctactcagag 1381 aagatcatta aggccaacca cttggacaac atcatcacca tatttaaggg taaagtggaa 1441 gaggtggagc tgcctgtgga gaaggtggac atcatcatca gcgagtggat gggctactgt 1501 ctgttctatg agtccatgct caacacggtg atctttgcca gggacaagtg gctgaaacct 1561 ggagggctta tgtttccaga ccgggcagct ttgtacgtgg tagcgattga agacagacag 1621 tacaaggact tcaaaatcca ctggtgggag aatgtctatg gctttgacat gacctgcatc 1681 cgggacgtgg ccatgaagga gcctctagtg gacatcgtgg atccaaagca agtggtgacc 1741 aatgcctgtt tgataaagga ggtggacatt tacacagtga agacggaaga gctatcgttc 1801 acatctgcat tctgcctgca gatacagcgc aacgactacg tccacgccct ggtcacctat 1861 tttaatattg aatttaccaa gtgccacaag aaaatggggt tttccacagc ccctgatgct 1921 ccctacaccc actggaagca gaccgtcttc tacttggaag attacctcac tgtccggagg 1981 ggggaggaaa tctacgggac catatccatg aagccaaatg ccaaaaatgt gcgagacctc 2041 gatttcacag tagacttgga ttttaaggga cagctgtgtg aaacatctgt atctaatgac 2101 tacaaaatgc gtctcgagga acaaaaacta atatcagagg aagatttgga acagaaacta 2161 atcagtgaag aagatttaga gcaaaagtta atttccgagg aggacttatg agggcccgtt 2221 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc 2281 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat 2341 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg 2401 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc 2461 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc 2521 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 2581 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 2641 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 2701 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 2761 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 2821 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 2881 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 2941 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca 3001 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 3061 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 3121 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 3181 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc 3241 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt 3301 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac 3361 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 3421 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 3481 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg 3541 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 3601 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 3661 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 3721 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 3781 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 3841 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc 3901 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 3961 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 4021 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 4081 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 4141 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg 4201 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga 4261 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa 4321 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 4381 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 4441 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt 4501 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 4561 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 4621 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 4681 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 4741 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 4801 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 4861 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 4921 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 4981 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 5041 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 5101 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 5161 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 5221 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 5281 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 5341 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 5401 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag 5461 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 5521 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 5581 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 5641 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 5701 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 5761 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 5821 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 5881 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 5941 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 6001 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 6061 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 6121 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 6181 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 6241 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 6301 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 6361 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 6421 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 6481 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 6541 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 6601 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct 6661 cccgatcccc tatggt //