LOCUS V027988 7570 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V027988 VERSION V027988 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 931..3006 /label="PRMT7(NM_019023)" /note="PRMT7(NM_019023)" /gene="PRMT7" CDS 3013..3102 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" polyA_signal 3137..3361 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3407..3835 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3849..4178 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4245..5039 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5213..5334 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5383..5399) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5407..5423 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5431..5461) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5476..5497 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5785..6370) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6541..7401) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7402..7506) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cactagtcca gtgtggtgga attcgccacc atgaagatct tctgcagtcg ggccaatccg 961 accacggggt ctgtggagtg gctggaggag gatgaacact atgattacca ccaggagatt 1021 gcaaggtcat cttatgcaga tatgctacat gacaaagaca gaaatgtaaa atactaccaa 1081 ggtatccggg ctgccgtgag cagggtgaag gacagaggac agaaggcctt ggttctcgac 1141 attggcactg gcacgggact cttgtcaatg atggcggtca cagcaggtgc cgacttctgc 1201 tatgccatcg aggttttcaa gcctatggct gatgctgctg tgaagattgt ggagaaaaat 1261 ggctttagtg ataagattaa ggttatcaac aagcattcca ccgaggtgac tgtaggtcca 1321 gagggtgaca tgccatgccg tgccaacatc ctggtcacag agttgtttga cacagagctg 1381 atcggggagg gggcgctgcc ctcctatgag cacgcacaca ggcatctcgt ggaggaaaat 1441 tgtgaggccg tgccccacag agccaccgtc tatgcacagc tggtggagtc cgggaggatg 1501 tggtcgtgga acaagctatt tcccatccac gtgcagacca gcctcggaga gcaggtcatc 1561 gtccctcccg ttgacgtgga gagctgccct ggcgcaccct ctgtctgtga cattcagctg 1621 aaccaggtgt caccagccga ctttacagtc ctcagcgatg tgctgcccat gttcagcata 1681 gacttcagca agcaagtcag tagctcagca gcctgccata gcaggcggtt tgaacctctg 1741 acatctggcc gagctcaggt ggttctctcg tggtgggaca ttgaaatgga ccctgagggg 1801 aagatcaagt gcaccatggc ccccttctgg gcacactcag acccagagga gatgcagtgg 1861 cgggaccact ggatgcagtg tgtgtacttc ctgccacaag aggagcctgt ggtgcagggc 1921 tcagcgctct atctggtagc ccaccacgat gactactgcg tatggtacag cctgcagagg 1981 accagccctg aaaagaatga gagagtccgc cagatgcgcc ccgtgtgtga ctgccaggct 2041 cacctgctct ggaaccggcc tcggtttgga gagatcaatg accaggacag aactgatcga 2101 tacgtccagg ctctgaggac cgtgctgaag ccagacagcg tgtgcctgtg tgtcagcgat 2161 ggcagcctgc tctccgtgct ggcccatcac ctgggggtgg agcaggtgtt tacagtcgag 2221 agttcagcag cttctcacaa actgttgaga aaaatcttca aggctaacca cttggaagat 2281 aaaattaaca tcatagagaa acggccggaa ttattaacaa atgaggacct acagggcaga 2341 aaggtctctc tcctcctggg cgagccgttc ttcactacca gcctgctgcc gtggcacaac 2401 ctctacttct ggtacgtgcg gaccgctgtg gaccagcacc tggggccagg tgccatggtg 2461 atgccccagg cagcctcgct gcacgctgtg gttgtggagt tcagggacct gtggcggatc 2521 cggagcccct gtggtgactg cgaaggcttc gacgtgcaca tcatggacga catgattaag 2581 cgtgccctgg acttcaggga gagcagggaa gctgagcccc acccgctgtg ggagtaccca 2641 tgccgcagcc tctccgagcc ctggcagatc ctgacctttg acttccagca gccggtgccc 2701 ctgcagcccc tgtgtgccga gggcaccgtg gagctcagaa ggcccgggca gagccacgca 2761 gcggtgctat ggatggagta ccacctgacc ccggagtgca cgctcagcac tggcctcctg 2821 gagcctgcag accccgaggg gggctgctgc tggaaccccc actgcaagca ggccgtctac 2881 ttcttcagcc ctgccccaga tcccagagca ctgctgggtg gcccacggac tgtcagctat 2941 gcagtggagt ttcaccccga cacaggcgac atcatcatgg agttcaggca tgcagatacc 3001 ccagacctcg aggaacaaaa actaatatca gaggaagatt tggaacagaa actaatcagt 3061 gaagaagatt tagagcaaaa gttaatttcc gaggaggact tatgagggcc cgtttaaacc 3121 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc 3181 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa 3241 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac 3301 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg 3361 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc 3421 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 3481 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 3541 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 3601 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 3661 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 3721 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 3781 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc 3841 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta 3901 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 3961 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 4021 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 4081 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt 4141 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat 4201 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg 4261 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac 4321 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg 4381 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc 4441 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg 4501 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc 4561 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc 4621 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta 4681 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg 4741 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg 4801 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat 4861 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc 4921 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta 4981 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag 5041 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt 5101 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg 5161 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt 5221 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 5281 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 5341 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt 5401 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 5461 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 5521 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 5581 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 5641 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 5701 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 5761 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 5821 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 5881 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 5941 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 6001 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 6061 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 6121 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 6181 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 6241 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 6301 acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 6361 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 6421 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 6481 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 6541 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 6601 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 6661 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 6721 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 6781 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 6841 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 6901 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 6961 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 7021 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 7081 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 7141 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 7201 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 7261 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 7321 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 7381 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 7441 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 7501 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat 7561 cccctatggt //