LOCUS V024837 7511 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V024837 VERSION V024837 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..3001 /label="PATZ1(NM_014323)" /note="PATZ1(NM_014323)" /gene="PATZ1" CDS 3008..3073 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 3111..3335 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3381..3809 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3823..4152 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4219..5013 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5187..5308 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5357..5373) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5381..5397 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5405..5435) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5450..5471 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5759..6344) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6515..7375) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7376..7480) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggagcggg tgaacgacgc 961 ttcgtgcggc ccgtctggct gctacacata ccaggtgagc agacacagca cggagatgct 1021 gcacaacctg aaccagcagc gcaaaaacgg cgggcgcttc tgcgacgtgc tcttgcgggt 1081 aggcgacgag agcttcccag cgcaccgcgc cgtgctggcc gcctgcagcg agtactttga 1141 gtcggtgttc agcgcccagt tgggcgacgg cggagctgcg gacgggggtc cggctgatgt 1201 agggggcgcg acggcagcac caggcggcgg ggccgggggc agccgggagc tggagatgca 1261 cactatcagc tccaaggtat ttggggacat tctggacttc gcctacactt cccgcatcgt 1321 ggtgcgcttg gagagctttc ccgaactcat gacggccgcc aagttcctgc tgatgaggtc 1381 ggttatcgag atctgccagg aagtcatcaa acagtccaac gtacagatcc tggtaccccc 1441 tgcccgcgcc gatataatgc tctttcgccc ccctgggacc tcggacttgg gcttcccttt 1501 ggacatgacc aacggggcag ccttggcagc caacagcaat ggcatcgccg gcagcatgca 1561 gccagaggag gaggcagctc gggcggctgg tgcagccatt gcaggccaag cctctttgcc 1621 tgtgttacct ggggtggacc gcttgcccat ggtggctgga cccctatccc cccaactgct 1681 gacttcccca ttccccagtg tggcatccag tgcccctccc ctgactggca agcgaggccg 1741 gggccgccca aggaaggcca acctgctgga ctcaatgttt gggtccccag ggggcctgag 1801 ggaggcaggc atccttccat gcggtctatg tggtaaggtg ttcactgatg ccaaccggct 1861 ccggcagcac gaggcccagc acggtgtcac cagcctccag ctgggctaca tcgaccttcc 1921 tcctccgagg ctgggtgaga atgggctacc catctctgaa gaccccgacg gcccccgaaa 1981 gaggagccgg accaggaagc aggtggcttg tgagatctgc ggcaagatct tccgtgatgt 2041 gtatcatctt aaccggcaca agctgtccca ctctggggag aagccctact cctgccctgt 2101 gtgtgggttg cggttcaaga gaaaagaccg catgtcctac catgtgcggt cccatgatgg 2161 gtccgtgggc aagccttaca tctgccagag ctgtgggaaa ggcttctcca ggcctgatca 2221 cttgaacgga catatcaagc aggtgcacac ttctgagcgg cctcacaagt gtcagacctg 2281 caatgcttct tttgccaccc gagaccgtct gcgctcccac ctggcctgtc atgaagacaa 2341 ggtgccctgc caggtgtgtg ggaagtactt gcgggcagca tacatggcag accacctgaa 2401 gaagcacagc gaggggccca gcaacttctg cagtatctgt aaccgaggtt tctcctctgc 2461 ctcctactta aaggtccatg ttaaaaccca ccacggtgtt ccccttcccc aggtctccag 2521 gcaccaggag cccatcctga atgggggagc agcgttccac tgcgccagga cctatggcaa 2581 caaagaaggc cagaaatgct cacatcagga tccgattgag agctctgact cctatggtga 2641 cctctcagat gccagcgacc tgaagacgcc agagaagcag agtgccaatg gctctttctc 2701 ctgcgacatg gcagtcccca aaaacaaaat ggagtctgat ggggagaaga agtacccatg 2761 ccctgaatgt gggagcttct tccgctctaa gtcctacttg aacaaacaca tccagaaggt 2821 gcatgtccgg gctctcgggg gccccctggg ggacctgggc cctgcccttg gctcaccttt 2881 ctctcctcag cagaacatgt ctctcctcga gtcctttggg tttcagattg ttcagtcggc 2941 atttgcgtca tctttagtag atcctgaggt tgaccagcag cccatggggc ctgaagggaa 3001 actcgaggac tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga 3061 tgacgatgac aagtagtgag ggcccgttta aacccgctga tcagcctcga ctgtgccttc 3121 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 3181 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 3241 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 3301 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg 3361 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 3421 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 3481 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 3541 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 3601 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 3661 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 3721 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 3781 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt 3841 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 3901 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 3961 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 4021 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 4081 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 4141 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca 4201 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct 4261 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc 4321 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc 4381 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc 4441 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg 4501 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc 4561 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac 4621 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat 4681 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc 4741 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg 4801 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg 4861 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc 4921 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc 4981 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg 5041 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa 5101 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc 5161 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat 5221 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 5281 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga 5341 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 5401 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 5461 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 5521 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 5581 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 5641 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 5701 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 5761 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 5821 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 5881 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 5941 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 6001 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 6061 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 6121 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 6181 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 6241 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt 6301 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 6361 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 6421 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 6481 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 6541 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 6601 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 6661 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 6721 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 6781 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 6841 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 6901 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 6961 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 7021 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 7081 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 7141 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 7201 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 7261 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 7321 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 7381 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 7441 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 7501 cacctgacgt c //