LOCUS V046799 7792 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V046799 VERSION V046799 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 964..3282 /label="PANK4(NM_018216)" /note="PANK4(NM_018216)" /gene="PANK4" CDS 3289..3354 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 3392..3616 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3662..4090 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4104..4433 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4500..5294 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5468..5589 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5638..5654) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5662..5678 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5686..5716) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5731..5752 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6040..6625) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6796..7656) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7657..7761) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc 961 accatggcgg agtgtggagc gagcggcagc gggagcagcg gggacagtct ggacaagagc 1021 atcacgctgc cccccgacga gatcttccgc aacctggaga acgccaagcg cttcgccatc 1081 gacataggcg ggtcgttaac caagctggcc tactattcaa cggtacagca caaagtcgcc 1141 aaggtgcggt ctttcgacca ctccggaaag gacacagaac gtgaacatga gccgccctat 1201 gagatttcag ttcaagaaga gatcactgct cgactgcact tcattaagtt tgagaatacc 1261 tacatcgaag cctgcctgga cttcatcaaa gaccatctcg tcaacacaga gaccaaggtc 1321 atccaggcga ccgggggcgg ggcctacaag ttcaaagacc tcatcgaaga gaagctgcgg 1381 ctgaaagtcg acaaggagga cgtgatgacg tgcctgatta aggggtgcaa cttcgtgctc 1441 aagaacatcc cccatgaggc cttcgtgtac cagaaggatt ccgaccctga gttccggttc 1501 cagaccaacc acccccacat tttcccctat cttcttgtca atatcggctc tggagtctcc 1561 atcgtgaagg tggagacgga ggacaggttc gagtgggtcg gcggcagctc cattggaggc 1621 ggcaccttct gggggcttgg cgctctgctc accaaaacga agaagtttga cgagctcctg 1681 cacctggcct cgaggggcca gcacagcaat gtggacatgc tggtgcggga cgtctacggc 1741 ggcgcccacc agactctcgg gctgagcggg aacctcatcg ccagcagctt cgggaagtcg 1801 gccaccgccg accaagagtt ctccaaagaa gacatggcga agagcctgct gcacatgatc 1861 agcaacgaca ttgggcagct ggcctgcctc cacgcacggc tgcacagcct ggaccgcgtg 1921 tactttggag gcttctttat ccggggccac cccgtgacca tgcgcaccat cacctatagc 1981 atcaacttct tctccaaggg ggaagtgcag gcgctgtttc tgaggcacga aggctacctg 2041 ggagccatcg gagcgttcct gaaaggagct gagcaggaca atcctaacca gtacagctgg 2101 ggagagaact atgcaggcag ctccgggctg atgagtgcat cacccgagct cggcccggcg 2161 cagcgggcgc ggagtggcac ttttgacttg ctggaaatgg accggctgga gaggccactg 2221 gttgacctgc cgctcctcct ggacccgccc tcctacgtgc ccgacacggt ggacctcacc 2281 gatgacgctc tggcccgaaa atactggctc acctgctttg aggaggccct ggacggggta 2341 gtgaagcgcg cagtggcgag ccagccagac tctgtggatg cagccgagag ggcggagaag 2401 ttccggcaga agtactggaa caagcttcag accctgaggc agcagccctt cgcctatggg 2461 accctgaccg tgcgcagcct gctggacacc agggagcact gtctgaacga gttcaacttc 2521 ccggatccct actccaaagt gaagcagcgg gagaatggcg tggcgctgag gtgcttcccc 2581 ggggtcgtgc gctccctgga cgcgctgggc tgggaggaac ggcagctggc gctggtgaaa 2641 ggcctcctgg cggggaatgt cttcgactgg ggggccaaag ccgtgtctgc tgtccttgaa 2701 tccgacccct actttgggtt tgaagaagca aagaggaagt tacaagaaag accctggctc 2761 gtggattcct acagcgagtg gcttcagaga ttaaaggggc cccctcataa atgtgcctta 2821 attttcgcag ataacagtgg aatagacatc attttgggag tcttcccctt tgtcagggag 2881 ctactcctta gagggacaga ggtcatcctg gcgtgcaact caggccccgc cctgaacgac 2941 gtgacccaca gcgagtccct catcgtggca gagcgtattg cgggcatgga ccctgtcgtg 3001 cactctgcgc tccaggaaga gaggctgctg ctggtgcaga cgggctccag ctccccgtgc 3061 ctcgacctca gccgcctgga taaggggctg gccgcactgg tgcgggagcg tggcgcggat 3121 ctggtggtca tcgagggcat gggccgtgct gtccacacaa actaccacgc agccctgcgc 3181 tgcgagagcc tcaagctggc cgtcatcaag aacgcgtggc tggccgagcg gctgggcggc 3241 cggctcttca gcgtcatctt caagtacgag gtcccagccg agctcgagga ctacaaagac 3301 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagtga 3361 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt 3421 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc 3481 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt 3541 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat 3601 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc 3661 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 3721 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 3781 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 3841 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 3901 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 3961 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 4021 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 4081 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc 4141 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 4201 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 4261 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 4321 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg 4381 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 4441 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca 4501 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 4561 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 4621 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 4681 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 4741 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 4801 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 4861 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 4921 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 4981 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg 5041 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 5101 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 5161 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 5221 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 5281 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct 5341 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 5401 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 5461 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 5521 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 5581 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 5641 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 5701 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 5761 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 5821 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 5881 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 5941 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 6001 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 6061 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 6121 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 6181 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 6241 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 6301 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 6361 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 6421 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 6481 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 6541 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat 6601 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 6661 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 6721 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 6781 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 6841 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 6901 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 6961 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 7021 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 7081 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 7141 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 7201 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 7261 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 7321 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 7381 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 7441 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 7501 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 7561 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 7621 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 7681 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 7741 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc //