LOCUS       V039992                 8754 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039992
VERSION     V039992
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             955..4269
                     /label="Otud4(NM_001191700)"
                     /note="Otud4(NM_001191700)"
                     /gene="Otud4"
     CDS             4291..4320
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     polyA_signal    4349..4573
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4619..5047
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5061..5390
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5457..6251
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6427..6548
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6597..6613)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6621..6637
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6645..6675)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6690..6711
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6999..7587)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7758..8618)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8619..8723)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattcgc caccatggag
      961 gcagctgtcg gcgcgcccga cggcgtggac cagggcggcg cgggaccgct ggaggatgcg
     1021 acgcccatgg acgcctatct gcgcaagctg ggattgtatc ggaaattggt cgccaaggac
     1081 gggtcgtgct tgttccgggc tgtggccgag caggtattac actctcagtc tcgacatgtg
     1141 gaagttagaa tggcttgtat tcgctacctt cgagataaca gagagaaatt tgaagcgttt
     1201 atagaagggt catttgaaga atatttaaaa cgtttggaaa acccacagga atgggtagga
     1261 caagtggaga taagcgccct ctctcttatg tacaggaaag attttgtaat atatcaggag
     1321 ccaaatgttt ctccttcgca tgtaactgaa aataattttc ctgagaaggt gttactgtgc
     1381 ttttcgaatg gaaatcatta tgacattgtc tatcctataa catacagaga tagttctgct
     1441 gtgtgtcagt ctctccttta tgaattgctg tatgagaagg tatttaaaac tgacgtcagt
     1501 aaaatcatga tgggactaga aacttctgaa gtggctgatg aaagcaacag tgaaatatca
     1561 gattctgagg atgacagttg caagagtaaa agcactgctg ctactgatgt gaatggattt
     1621 aagtcctcag gcagtgagaa ccctaagaac catgggaact cagctgacct tcctttgtcc
     1681 agaaaggttc ttaagtccct cagcccagca gtctatagaa atgtggagta tgaaatttgg
     1741 ttgaagtcta agcaagctca acaaaaacgt gattattcca ttgctgctgg cttacaatat
     1801 gaaggaggag agagatgcca tgttagattg gatcataatg ggaagttatc taatgcagac
     1861 attcatgggg ttcactctga gaatggaccg gttttgtctg aagaactggg gaaaaagcaa
     1921 acaccgaaga acctcaagcc acctccccca gaaagctgga acacggtgtc cggaaagaag
     1981 atgaaaaaac ctaattctgg gcaaaatttc cattcagata cggattacag agggccaaag
     2041 aatctaaaca agccaatcaa agccccgtct gcactacctc ctcgcctgca gcatcctcca
     2101 gcgggtgtaa ggcagcacgc gctctccagt cattctacgg ggccccagtc tcagaaatcc
     2161 tccagtgagc ataagaatct aagtcgggtg ccatcacaga tcacaagaaa acctgatcgt
     2221 gaaagagctg aggactttga tcatgtgagt cgtgaatctt actattttgg cctctcgcca
     2281 gaagaacgca gagagaagca agctattgaa gagtctcggc tactctatga gattcagaac
     2341 cgggatgaac aggcttttcc ggctctttct agttcatcag tcagtcagtc acctcagaat
     2401 agcaacgcgt gcgtcccaag gaagtcttca catgcgaggg acaggaaagg aagcatgcgg
     2461 agagtagatg cagaggaacg gaaggacaaa gactctctac gtggccatac tcacatggat
     2521 aaaaaacccg agccaagcac actggagatc agcgatgata aatgtacaag agtttcatca
     2581 ccatctaagt caaagaaaga gtgcccatct cctgtagaac aaaagccagc agaacatata
     2641 cctttgtcaa atccagcccc cctcctagtc tctccagaag ttcatctcac tcctgcggtg
     2701 ccttctttac cagccactgt gccagcctgg ccaagtgaac ccacaacttt cggaccaaca
     2761 ggtgtccctg ctcagattcc cattttgtca gtgacacaga ccactggacc tgatgctgcc
     2821 gtgtcacaag cgcatttaac accctctccg gttcctgtgt caattcaggc agttaaccag
     2881 cccttgatgc ctttgcctca gacaatgagc ctctatcaag acccactcta tcctgggttt
     2941 ccttgtagtg agaagggaga tcgagccatt gcaccaccct attcactgtg tcagaccggc
     3001 gaggacctgc ctaaagataa gaatattctt cgattcttct tcaatcttgg tgtaaaggca
     3061 tatagttgcc ctatgtgggc cccacactct tacctgtatc ctctgcacca ggcgtacatg
     3121 gcagcctgca gggtgtaccc aaaggtccct gttcctgtgt atcctcagag tacttggttc
     3181 caagaagccc ctcctgctca gagtgaaagt gactgcccct gtgctgacgc ccactactct
     3241 atgcaccctg aggccagtgt taatggccag atgccacagg cagagatcgg accacctgcg
     3301 ttttcttccc ctctggttat ccctccatct caggtgtctg aaggtcatgg acagttgtct
     3361 taccaacctg aactggagtc tgagagccca gggcagcttc ttcacgctga gtatgaagag
     3421 tcacttagcg gcaagaacat gtacccacag cagtcctttg ggcctaatcc attcttaggt
     3481 cctgttccca ttgcacctcc tttcttccct catgtttggt atgggtatcc ttttcaggga
     3541 tttgtggaaa atcctgtaat gaggcaaaat attgtcctgc cccctgatga taaaggagag
     3601 ttggatttgc ctttggaaaa tctagatctg tctaaagagt gcgattctgt ctcagcagta
     3661 gacgagttcc cagaagccag agttgaaggc gcacattctc tctctgaagc aagtgtgagc
     3721 agcaagcatg aaggccgggt ggagcagtcg tcccagacgc ggaaggcaga catagacttg
     3781 tcttcaggtt cttctggagt ggagggaaag gctcaccccc ctactcagat tctaaaccga
     3841 gagagagagg ctgtgtctgc tgaacctgag cctaagagga ccattcaaag tctgaaagaa
     3901 aaaccagaga aagtcaaaga ccccaagact gctgccgatg tgctcagctc tggggccaac
     3961 tctgtggata gattgcaaag accaaaagaa gagagttcag aagatgagaa cgaagtgtct
     4021 aatattttga gaagtggcag atccaagcag ttttataatc aaacttatgg aagcaggaag
     4081 tacaaaagtg attggggctc ttctggtaga ggtggctatc aacatgtgag aggcgaggag
     4141 tcctggaaag ggcagcctaa cagaagccgg gatgaaggtt atcagtacca tcgacatgtt
     4201 agaggacgcc cttacagggg agataggagg aggtcaggga tgggagatgg ccacagggga
     4261 caacacactc tcgagtctag agggcccttc gaacaaaaac tcatctcaga agaggatctg
     4321 tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     4381 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4441 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     4501 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     4561 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     4621 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4681 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4741 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4801 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4861 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4921 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4981 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5041 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     5101 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     5161 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     5221 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5281 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     5341 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     5401 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     5461 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     5521 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     5581 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     5641 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     5701 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     5761 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     5821 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     5881 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     5941 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     6001 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     6061 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     6121 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     6181 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     6241 agttcttctg agcgggactc tggggttcgc gaaatgaccg accaagcgac gcccaacctg
     6301 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     6361 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     6421 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6481 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6541 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     6601 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6661 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6721 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6781 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6841 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6901 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6961 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7021 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7081 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7141 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7201 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7261 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7321 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7381 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7441 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7501 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7561 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7621 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7681 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7741 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7801 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7861 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7921 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7981 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8041 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8101 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8161 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8221 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8281 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8341 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8401 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8461 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8521 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8581 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8641 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8701 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
