LOCUS       V018404                 8598 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018404
VERSION     V018404
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             895..4032
                     /label="Ogt(NM_139144)"
                     /note="Ogt(NM_139144)"
                     /gene="Ogt"
     CDS             4033..4062
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     polyA_signal    4165..4389
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4435..4863
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4877..5206
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5273..6067
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6241..6362
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6411..6427)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6435..6451
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6459..6489)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6504..6525
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6813..7398)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7569..8429)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8430..8534)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg ccacatggcg
      901 tcttccgtgg gcaacgtggc cgacagtaca gaaccaacga aacgtatgct ttccttccaa
      961 gggttagctg agttggcaca tcgagaatat caggcaggag attttgaggc agctgagaga
     1021 cactgcatgc agctctggag acaagagcct gacaatactg gtgttctttt attactttca
     1081 tctatacact tccagtgtcg aaggctggac agatctgctc attttagcac cttggcaatt
     1141 aaacagaatc cccttctagc agaagcctat tcgaatttgg gaaatgtgta caaggaaaga
     1201 gggcagttgc aggaagcaat cgagcattat cgacatgcct tgcggctgaa gcctgatttc
     1261 attgatggtt atattaacct ggcagcagcc ttggtagcag caggtgacat ggaaggagca
     1321 gtacaagcct atgtctctgc tcttcagtac aatcctgatt tgtactgtgt tcgcagtgac
     1381 ctggggaacc tgctcaaagc cctgggtcgc ttggaagaag ccaaggcatg ttatttgaaa
     1441 gcaattgaga cgcaaccaaa ctttgcagta gcctggagta atctcggctg tgttttcaat
     1501 gcacaagggg agatttggct ggctattcat cactttgaaa aggctgtcac ccttgaccca
     1561 aattttctgg atgcttatat caatttagga aatgtcttga aagaggcacg catttttgac
     1621 agagctgtcg cagcttatct tcgtgcctta agtttgagcc caaatcatgc ggtggtgcac
     1681 ggcaacctgg cttgtgtgta ctacgagcaa ggcctaatag acctggccat tgatacctac
     1741 aggagagcta tcgaactgca accccatttc cccgatgctt actgcaacct agcaaatgct
     1801 ctcaaagaga agggcagtgt tgctgaagca gaagattgtt ataacacagc tcttcgtctg
     1861 tgtcctactc atgcagactc tttgaataac cttgccaaca tcaaacggga acagggcaac
     1921 attgaagagg cagttcgcct gtatcgcaaa gcattagaag tcttcccaga gtttgctgct
     1981 gcacattcca atttagcaag tgtactgcaa cagcagggca agctgcagga agcactgatg
     2041 cactataaag aagccatacg aattagtcct acatttgctg atgcttattc caatatggga
     2101 aacactctaa aggagatgca ggatgtgcag ggcgctttgc agtgttatac tcgtgccatc
     2161 cagattaatc ctgcctttgc tgatgcacac agcaatctgg cctccattca caaggattca
     2221 gggaatatcc cagaagcaat agcttcttac cgcacagctc tgaaacttaa gcctgacttt
     2281 cctgatgctt attgtaactt ggctcattgc ctacagattg tctgtgattg gacagactat
     2341 gatgagcgga tgaagaaatt ggttagtatt gtagctgagc agctagagaa gaatagactg
     2401 ccttctgtcc atcctcacca tagcatgctg taccctcttt cccatggctt caggaaggct
     2461 attgcagaga ggcatgggaa tctctgcttg gataagatta atgtccttca taaaccacca
     2521 tatgaacatc caaaagactt gaagctcagt gatggccgat tgcgtgtagg ctatgtgagt
     2581 tctgacttcg ggaatcaccc tacttcacac cttatgcagt ctattccagg catgcataat
     2641 cctgataagt ttgaggtatt ctgctatgcc ttgagcccgg atgatggtac aaactttcga
     2701 gtgaaggtga tggcggaagc caatcatttc attgatcttt ctcagattcc ttgtaatgga
     2761 aaagcagccg accgcatcca ccaagatgga attcacatcc ttgtgaatat gaatgggtat
     2821 accaagggtg ctcggaatga gctctttgct cttaggccag ctcctattca ggccatgtgg
     2881 ctgggctacc ctgggactag tggtgcactg ttcatggatt acatcatcac tgatcaggaa
     2941 acttccccag ctgaagttgc agagcagtat tctgagaaac tggcttatat gccccatact
     3001 ttctttattg gtgatcatgc taatatgttc cctcacctga agaaaaaagc agtcatcgat
     3061 tttaaatcca atgggcacat ttatgataat cggatagttc tgaatggcat cgatctcaaa
     3121 gcatttctcg atagcctacc cgatgtgaag attgtcaaga tgaaatgtcc tgatggaggt
     3181 gacaatccag acagcagtaa cacagctctt aatatgcccg ttattcccat gaatacgatt
     3241 gcagaagcag taattgaaat gattaacaga gggcagattc agataacaat taacggattc
     3301 agtattagca atggactggc gactacacag attaataata aggctgcaac cggagaggaa
     3361 gttccccgta ccattattgt aaccacccgt tcccagtatg ggctaccaga agatgccatt
     3421 gtgtactgta actttaatca gttatataaa attgacccat ctaccctgca gatgtgggca
     3481 aatattctga aacgtgtgcc taacagcgtg ctttggctgt tgcgttttcc agcagtagga
     3541 gaacccaata ttcaacaata tgcacaaaat atgggccttc cccagaaccg tatcattttc
     3601 tcacctgtgg ctcctaaaga ggagcatgtc aggagaggtc agctggctga tgtctgcctg
     3661 gatactcctt tgtgtaatgg acacaccaca gggatggatg ttctctgggc aggaacaccc
     3721 atggtgacta tgccaggaga gactcttgcc tctcgagttg cagcttctca gcttacttgt
     3781 ctaggatgtc tcgagctcat tgctaaaagc agacaggaat atgaagacat agctgtgaaa
     3841 ctgggaaccg atctagaata cctgaagaaa attcgtggca aagtctggaa acagagaata
     3901 tctagccctc tgttcaacac caaacaatac acaatggaat tagagcgact ttatctgcag
     3961 atgtgggagc attatgcagc tggcaacaaa cctgaccaca tgattaagcc tgttgaagtc
     4021 accgagtcag ccgaacaaaa actcatctca gaagaggatc tgtaaggatc cactagtcca
     4081 gtgtggtgga attctgcaga tatccagcac agtggcggcc gctcgagtct agagggcccg
     4141 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     4201 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     4261 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     4321 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     4381 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     4441 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4501 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4561 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4621 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     4681 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4741 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     4801 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4861 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4921 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4981 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     5041 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5101 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     5161 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     5221 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     5281 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     5341 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     5401 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     5461 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     5521 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5581 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5641 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5701 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5761 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     5821 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5881 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5941 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     6001 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     6061 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     6121 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     6181 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     6241 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6301 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6361 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     6421 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6481 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6541 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6601 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6661 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6721 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6781 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6841 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6901 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6961 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7021 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7081 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7141 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7201 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7261 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7321 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc
     7381 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7441 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7501 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7561 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7621 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7681 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7741 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7801 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7861 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7921 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7981 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8041 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8101 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8161 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8221 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8281 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8341 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8401 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8461 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8521 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     8581 ctcccgatcc cctatggt
//
