LOCUS       V029770                 8629 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029770
VERSION     V029770
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..4068
                     /label="OGT(NM_181672)"
                     /note="OGT(NM_181672)"
                     /gene="OGT"
     CDS             4075..4101
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4105..4131
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4135..4161
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    4196..4420
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4466..4894
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4908..5237
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5304..6098
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6272..6393
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6442..6458)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6466..6482
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6490..6520)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6535..6556
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6844..7429)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7600..8460)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8461..8565)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggcgtctt ccgtgggcaa cgtggccgac
      961 agcacagaac caacgaaacg tatgctttcc ttccaagggt tagctgagtt ggcacatcga
     1021 gaatatcagg caggagattt tgaggcagct gagagacact gcatgcagct ctggagacaa
     1081 gagccagaca atactggtgt gcttttatta ctttcatcta tacacttcca gtgtcgaagg
     1141 ctggacagat ctgctcactt tagcactctg gcaattaaac agaaccccct tctggcagaa
     1201 gcttattcga atttggggaa tgtgtacaag gaaagagggc agttgcagga ggcaattgag
     1261 cattatcgac atgcattgcg tctcaaacct gatttcatcg atggttatat taacctggca
     1321 gccgccttgg tagcagcggg tgacatggaa ggggcagtac aagcttacgt ctctgctctt
     1381 cagtacaatc ctgatttgta ctgtgttcgc agtgacctgg ggaacctgct caaagccctg
     1441 ggtcgcttgg aagaagccaa ggcatgttat ttgaaagcaa ttgagacgca accgaacttt
     1501 gcagtagctt ggagtaatct tggctgtgtt ttcaatgcac aaggggaaat ttggcttgca
     1561 attcatcact ttgaaaaggc tgtcaccctt gacccaaact ttctggatgc ttatatcaat
     1621 ttaggaaatg tcttgaaaga ggcacgcatt tttgacagag ctgtggcagc ttatcttcgt
     1681 gccctaagtt tgagtccaaa tcacgcagtg gtgcacggca acctggcttg tgtatactat
     1741 gagcaaggcc tgatagatct ggcaatagac acctacaggc gggctatcga actacaacca
     1801 catttccctg atgcttactg caacctagcc aatgctctca aagagaaggg cagtgttgct
     1861 gaagcagaag attgttataa tacagctctc cgtctgtgtc ccacccatgc agactctctg
     1921 aataacctag ccaatatcaa acgagaacag ggaaacattg aagaggcagt tcgcttgtat
     1981 cgtaaagcat tagaagtctt cccagagttt gctgctgccc attcaaattt agcaagtgta
     2041 ctgcagcagc agggaaaact gcaggaagct ctgatgcatt ataaggaggc tattcgaatc
     2101 agtcctacct ttgctgatgc ctactctaat atgggaaaca ctctaaagga gatgcaggat
     2161 gttcagggag ccttgcagtg ttatacgcgt gccatccaaa ttaatcctgc atttgcagat
     2221 gcacatagca atctggcttc cattcataag gattcaggga atattccaga agccatagct
     2281 tcttaccgca cggctctgaa acttaagcct gattttcctg atgcttattg taacttggct
     2341 cattgcctgc agattgtctg tgattggaca gactatgatg agcgaatgaa gaagttggtc
     2401 agtattgtgg ctgaccagtt agagaagaat aggttgcctt ctgtgcatcc tcatcatagt
     2461 atgctatatc ctctttctca tggcttcagg aaggctattg ctgagaggca cggcaacctg
     2521 tgcttagata agattaatgt tcttcataaa ccaccatatg aacatccaaa agacttgaag
     2581 ctcagtgatg gtcggctgcg tgtaggatat gtgagttccg actttgggaa tcatcctact
     2641 tctcacctta tgcagtctat tccaggcatg cacaatcctg ataaatttga ggtgttctgt
     2701 tatgccctga gcccagacga tggcacaaac ttccgagtga aggtgatggc agaagccaat
     2761 catttcattg atctttctca gattccatgc aatggaaaag cagctgatcg catccatcag
     2821 gatggaattc atatccttgt aaatatgaat ggctatacta agggcgctcg aaatgagctt
     2881 tttgctctca ggccagctcc tattcaggca atgtggctgg gataccctgg gacgagtggt
     2941 gcgcttttca tggattatat tatcactgat caggaaactt cgccagctga agttgctgag
     3001 cagtattccg agaaattggc ttatatgccc cacacttttt ttattggtga tcatgctaat
     3061 atgttccctc acctgaagaa aaaagcagtc atcgatttta agtccaatgg gcacatttat
     3121 gacaatcgga tagttctgaa tggcatcgac ctcaaagcat ttcttgatag tctaccagat
     3181 gtgaaaattg tcaagatgaa gtgtcctgat ggaggagaca atgcagatag cagtaacaca
     3241 gctcttaata tgcctgttat tcctatgaat actattgcag aagcagttat tgaaatgatt
     3301 aaccgaggac agattcaaat aacaattaat ggattcagta ttagcaatgg actggcaact
     3361 actcagatca acaataaggc tgcaactgga gaggaggttc cccgtaccat tattgtaacc
     3421 acccgttctc agtacgggtt accagaagat gccatcgtat actgtaactt taatcagttg
     3481 tataaaattg acccttctac tttgcagatg tgggcaaaca ttctgaagcg tgttcccaat
     3541 agtgtactct ggctgttgcg ttttccagca gtaggagaac ctaatattca acagtatgca
     3601 caaaacatgg gcctgcccca gaaccgtatc attttttcac ctgttgctcc taaagaggaa
     3661 cacgtcagga gaggccagct ggctgatgtc tgcttggaca ctccactctg taatgggcac
     3721 accacaggga tggatgtcct ctgggcaggg acccccatgg tgactatgcc aggagagact
     3781 cttgcttctc gagttgcagc atcccagctc acttgcttag gttgtcttga gcttattgct
     3841 aaaaacagac aagaatatga agacatagct gtgaagctgg gaactgatct agaatacctg
     3901 aagaaagttc gtggcaaagt ctggaagcaa agaatatcta gccctctgtt caacaccaaa
     3961 caatacacaa tggaactaga gcggctctat ctacagatgt gggagcatta tgcagctggc
     4021 aacaaacctg accacatgat taagcctgtt gaagtcactg agtcagcact cgagtaccca
     4081 tacgacgtac ctgattatgc aacttatcct tatgatgtac ctgattatgc tagctatcct
     4141 tatgatgtcc cagactacgc atgagggccc gtttaaaccc gctgatcagc ctcgactgtg
     4201 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     4261 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     4321 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     4381 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     4441 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4501 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4561 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4621 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4681 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4741 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4801 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4861 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     4921 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4981 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5041 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     5101 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     5161 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     5221 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
     5281 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     5341 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     5401 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     5461 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     5521 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     5581 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     5641 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     5701 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     5761 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     5821 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     5881 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     5941 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     6001 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     6061 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     6121 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     6181 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     6241 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     6301 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6361 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     6421 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6481 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6541 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6601 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6661 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6721 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6781 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6841 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6901 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6961 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7021 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7081 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7141 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7201 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7261 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7321 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7381 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7441 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7501 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7561 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7621 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7681 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7741 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7801 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7861 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7921 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7981 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8041 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8101 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8161 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8221 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8281 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8341 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     8401 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8461 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8521 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8581 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggt
//
