LOCUS       V023278                 7607 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023278
VERSION     V023278
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             941..3097
                     /label="OAS2(NM_016817)"
                     /note="OAS2(NM_016817)"
                     /gene="OAS2"
     CDS             3104..3169
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    3207..3431
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3477..3905
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3919..4248
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4315..5109
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5283..5404
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5453..5469)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5477..5493
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5501..5531)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5546..5567
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5855..6440)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6611..7471)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7472..7576)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgggaaatg gggagtccca
      961 gctgtcctcg gtgcctgctc agaagctggg ttggtttatc caggaatacc tgaagcccta
     1021 cgaagaatgt cagacactga tcgacgagat ggtgaacacc atctgtgacg tcctgcagga
     1081 acccgaacag ttccccctgg tgcagggagt ggccataggt ggctcctatg gacggaaaac
     1141 agtcttaaga ggcaactccg atggtaccct tgtcctcttc ttcagtgact taaaacaatt
     1201 ccaggatcag aagagaagcc aacgtgacat cctcgataaa actggggata agctgaagtt
     1261 ctgtctgttc acgaagtggt tgaaaaacaa tttcgagatc cagaagtccc ttgatgggtt
     1321 caccatccag gtgttcacaa aaaatcagag aatctctttc gaggtgctgg ccgccttcaa
     1381 cgctctgagc ttaaatgata atcccagccc ctggatctat cgagagctca aaagatcctt
     1441 ggataagaca aatgccagtc ctggtgagtt tgcagtctgc ttcactgaac tccagcagaa
     1501 gttttttgac aaccgtcctg gaaaactaaa ggatttgatc ctcttgataa agcactggca
     1561 tcaacagtgc cagaaaaaaa tcaaggattt accctcgctg tctccgtatg ccctggagct
     1621 gcttacggtg tatgcctggg aacaggggtg cagaaaagac aactttgaca ttgctgaagg
     1681 cgtcagaacc gtactggagc tgatcaaatg ccaggagaag ctgtgtatct attggatggt
     1741 caactacaac tttgaagatg agaccatcag gaacatcctg ctgcaccagc tccaatcagc
     1801 gaggccagta atcttggatc cagttgaccc aaccaataat gtgagtggag ataaaatatg
     1861 ctggcaatgg ctgaaaaaag aagctcaaac ctggttgact tctcccaacc tggataatga
     1921 gttacctgca ccatcttgga atgttctgcc tgcaccactc ttcacgaccc caggccacct
     1981 tctggataag ttcatcaagg agtttctcca gcccaacaaa tgcttcctag agcagattga
     2041 cagtgctgtt aacatcatcc gtacattcct taaagaaaac tgcttccgac aatcaacagc
     2101 caagatccag attgtccggg gaggatcaac cgccaaaggc acagctctga agactggctc
     2161 tgatgccgat ctcgtcgtgt tccataactc acttaaaagc tacacctccc aaaaaaacga
     2221 gcggcacaaa atcgtcaagg aaatccatga acagctgaaa gccttttgga gggagaagga
     2281 ggaggagctt gaagtcagct ttgagcctcc caagtggaag gctcccaggg tgctgagctt
     2341 ctctctgaaa tccaaagtcc tcaacgaaag tgtcagcttt gatgtgcttc ctgcctttaa
     2401 tgcactgggt cagctgagtt ctggctccac acccagcccc gaggtttatg cagggctcat
     2461 tgatctgtat aaatcctcgg acctcccggg aggagagttt tctacctgtt tcacagtcct
     2521 gcagcgaaac ttcattcgct cccggcccac caaactaaag gatttaattc gcctggtgaa
     2581 gcactggtac aaagagtgtg aaaggaaact gaagccaaag gggtctttgc ccccaaagta
     2641 tgccttggag ctgctcacca tctatgcctg ggagcagggg agtggagtgc cggattttga
     2701 cactgcagaa ggtttccgga cagtcctgga gctggtcaca caatatcagc agctctgcat
     2761 cttctggaag gtcaattaca actttgaaga tgagaccgtg aggaagtttc tactgagcca
     2821 gttgcagaaa accaggcctg tgatcttgga cccagccgaa cccacaggtg acgtgggtgg
     2881 aggggaccgt tggtgttggc atcttctggc aaaagaagca aaggaatggt tatcctctcc
     2941 ctgcttcaag gatgggactg gaaacccaat accaccttgg aaagtgccga caatgcagac
     3001 accaggaagt tgtggagcta ggatccatcc tattgtcaat gagatgttct catccagaag
     3061 ccatagaatc ctgaataata attctaaaag aaacttcctc gaggactaca aagaccatga
     3121 cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt agtgagggcc
     3181 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     3241 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     3301 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     3361 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     3421 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     3481 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     3541 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     3601 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     3661 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     3721 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     3781 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     3841 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     3901 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     3961 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4021 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4081 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4141 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     4201 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     4261 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     4321 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     4381 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     4441 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     4501 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     4561 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     4621 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     4681 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     4741 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     4801 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     4861 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     4921 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     4981 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     5041 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     5101 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     5161 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     5221 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     5281 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5341 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5401 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     5461 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     5521 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     5581 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     5641 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     5701 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     5761 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5821 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5881 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5941 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6001 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6061 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6121 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6181 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6241 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6301 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6361 gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc
     6421 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6481 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6541 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6601 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6661 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6721 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     6781 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6841 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6901 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     6961 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7021 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7081 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7141 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7201 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7261 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7321 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7381 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     7441 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     7501 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7561 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
