LOCUS V023278 7607 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V023278 VERSION V023278 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..3097 /label="OAS2(NM_016817)" /note="OAS2(NM_016817)" /gene="OAS2" CDS 3104..3169 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 3207..3431 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3477..3905 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3919..4248 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4315..5109 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5283..5404 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5453..5469) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5477..5493 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5501..5531) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5546..5567 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5855..6440) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6611..7471) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7472..7576) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgggaaatg gggagtccca 961 gctgtcctcg gtgcctgctc agaagctggg ttggtttatc caggaatacc tgaagcccta 1021 cgaagaatgt cagacactga tcgacgagat ggtgaacacc atctgtgacg tcctgcagga 1081 acccgaacag ttccccctgg tgcagggagt ggccataggt ggctcctatg gacggaaaac 1141 agtcttaaga ggcaactccg atggtaccct tgtcctcttc ttcagtgact taaaacaatt 1201 ccaggatcag aagagaagcc aacgtgacat cctcgataaa actggggata agctgaagtt 1261 ctgtctgttc acgaagtggt tgaaaaacaa tttcgagatc cagaagtccc ttgatgggtt 1321 caccatccag gtgttcacaa aaaatcagag aatctctttc gaggtgctgg ccgccttcaa 1381 cgctctgagc ttaaatgata atcccagccc ctggatctat cgagagctca aaagatcctt 1441 ggataagaca aatgccagtc ctggtgagtt tgcagtctgc ttcactgaac tccagcagaa 1501 gttttttgac aaccgtcctg gaaaactaaa ggatttgatc ctcttgataa agcactggca 1561 tcaacagtgc cagaaaaaaa tcaaggattt accctcgctg tctccgtatg ccctggagct 1621 gcttacggtg tatgcctggg aacaggggtg cagaaaagac aactttgaca ttgctgaagg 1681 cgtcagaacc gtactggagc tgatcaaatg ccaggagaag ctgtgtatct attggatggt 1741 caactacaac tttgaagatg agaccatcag gaacatcctg ctgcaccagc tccaatcagc 1801 gaggccagta atcttggatc cagttgaccc aaccaataat gtgagtggag ataaaatatg 1861 ctggcaatgg ctgaaaaaag aagctcaaac ctggttgact tctcccaacc tggataatga 1921 gttacctgca ccatcttgga atgttctgcc tgcaccactc ttcacgaccc caggccacct 1981 tctggataag ttcatcaagg agtttctcca gcccaacaaa tgcttcctag agcagattga 2041 cagtgctgtt aacatcatcc gtacattcct taaagaaaac tgcttccgac aatcaacagc 2101 caagatccag attgtccggg gaggatcaac cgccaaaggc acagctctga agactggctc 2161 tgatgccgat ctcgtcgtgt tccataactc acttaaaagc tacacctccc aaaaaaacga 2221 gcggcacaaa atcgtcaagg aaatccatga acagctgaaa gccttttgga gggagaagga 2281 ggaggagctt gaagtcagct ttgagcctcc caagtggaag gctcccaggg tgctgagctt 2341 ctctctgaaa tccaaagtcc tcaacgaaag tgtcagcttt gatgtgcttc ctgcctttaa 2401 tgcactgggt cagctgagtt ctggctccac acccagcccc gaggtttatg cagggctcat 2461 tgatctgtat aaatcctcgg acctcccggg aggagagttt tctacctgtt tcacagtcct 2521 gcagcgaaac ttcattcgct cccggcccac caaactaaag gatttaattc gcctggtgaa 2581 gcactggtac aaagagtgtg aaaggaaact gaagccaaag gggtctttgc ccccaaagta 2641 tgccttggag ctgctcacca tctatgcctg ggagcagggg agtggagtgc cggattttga 2701 cactgcagaa ggtttccgga cagtcctgga gctggtcaca caatatcagc agctctgcat 2761 cttctggaag gtcaattaca actttgaaga tgagaccgtg aggaagtttc tactgagcca 2821 gttgcagaaa accaggcctg tgatcttgga cccagccgaa cccacaggtg acgtgggtgg 2881 aggggaccgt tggtgttggc atcttctggc aaaagaagca aaggaatggt tatcctctcc 2941 ctgcttcaag gatgggactg gaaacccaat accaccttgg aaagtgccga caatgcagac 3001 accaggaagt tgtggagcta ggatccatcc tattgtcaat gagatgttct catccagaag 3061 ccatagaatc ctgaataata attctaaaag aaacttcctc gaggactaca aagaccatga 3121 cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt agtgagggcc 3181 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 3241 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 3301 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 3361 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 3421 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc 3481 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 3541 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 3601 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 3661 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 3721 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 3781 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 3841 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 3901 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 3961 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 4021 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 4081 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 4141 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta 4201 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga 4261 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt 4321 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat 4381 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag 4441 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac 4501 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac 4561 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc 4621 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg 4681 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag 4741 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat 4801 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag 4861 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc 4921 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg 4981 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg 5041 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag 5101 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat 5161 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc 5221 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc 5281 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 5341 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 5401 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 5461 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 5521 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 5581 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 5641 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 5701 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 5761 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 5821 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 5881 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 5941 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6001 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6061 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 6121 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 6181 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 6241 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 6301 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 6361 gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc 6421 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 6481 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 6541 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 6601 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 6661 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 6721 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 6781 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 6841 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 6901 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 6961 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 7021 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 7081 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 7141 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 7201 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 7261 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 7321 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 7381 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 7441 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 7501 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 7561 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc //