LOCUS V023281 6542 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V023281 VERSION V023281 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..2032 /label="OAS1(NM_002534)" /note="OAS1(NM_002534)" /gene="OAS1" CDS 2039..2104 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 2142..2366 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2412..2840 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2854..3183 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3250..4044 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4218..4339 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4388..4404) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4412..4428 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4436..4466) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4481..4502 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4790..5375) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5546..6406) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6407..6511) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgatggatc tcagaaatac 961 cccagccaaa tctctggaca agttcattga agactatctc ttgccagaca cgtgtttccg 1021 catgcaaatc aaccatgcca ttgacatcat ctgtgggttc ctgaaggaaa ggtgcttccg 1081 aggtagctcc taccctgtgt gtgtgtccaa ggtggtaaag ggtggctcct caggcaaggg 1141 caccaccctc agaggccgat ctgacgctga cctggttgtc ttcctcagtc ctctcaccac 1201 ttttcaggat cagttaaatc gccggggaga gttcatccag gaaattagga gacagctgga 1261 agcctgtcaa agagagagag cattttccgt gaagtttgag gtccaggctc cacgctgggg 1321 caacccccgt gcgctcagct tcgtactgag ttcgctccag ctcggggagg gggtggagtt 1381 cgatgtgctg cctgcctttg atgccctggg tcagttgact ggcggctata aacctaaccc 1441 ccaaatctat gtcaagctca tcgaggagtg caccgacctg cagaaagagg gcgagttctc 1501 cacctgcttc acagaactac agagagactt cctgaagcag cgccccacca agctcaagag 1561 cctcatccgc ctagtcaagc actggtacca aaattgtaag aagaagcttg ggaagctgcc 1621 acctcagtat gccctggagc tcctgacggt ctatgcttgg gagcgaggga gcatgaaaac 1681 acatttcaac acagcccagg gatttcggac ggtcttggaa ttagtcataa actaccagca 1741 actctgcatc tactggacaa agtattatga ctttaaaaac cccattattg aaaagtacct 1801 gagaaggcag ctcacgaaac ccaggcctgt gatcctggac ccggcggacc ctacaggaaa 1861 cttgggtggt ggagacccaa agggttggag gcagctggca caagaggctg aggcctggct 1921 gaattaccca tgctttaaga attgggatgg gtccccagtg agctcctgga ttctgctggt 1981 gagacctcct gcttcctccc tgccattcat ccctgcccct ctccatgaag ctctcgagga 2041 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga 2101 caagtagtga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca 2161 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 2221 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 2281 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 2341 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag 2401 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 2461 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 2521 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 2581 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 2641 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 2701 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 2761 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 2821 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 2881 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 2941 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 3001 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 3061 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 3121 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 3181 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga 3241 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag 3301 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc 3361 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg 3421 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc 3481 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg 3541 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct 3601 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg 3661 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat 3721 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc 3781 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg 3841 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc 3901 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct 3961 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat 4021 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga 4081 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct 4141 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg 4201 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata 4261 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 4321 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt 4381 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 4441 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 4501 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 4561 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 4621 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 4681 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 4741 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 4801 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 4861 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 4921 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 4981 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 5041 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 5101 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 5161 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 5221 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 5281 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca 5341 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 5401 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 5461 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 5521 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 5581 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 5641 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 5701 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 5761 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 5821 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 5881 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 5941 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 6001 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 6061 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 6121 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 6181 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 6241 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 6301 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 6361 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 6421 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 6481 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 6541 tc //