LOCUS       V037311                 8149 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037311
VERSION     V037311
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             874..1590
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1642..1668
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1675..3639
                     /label="NLRP10(NM_001391958)"
                     /note="NLRP10(NM_001391958)"
                     /gene="NLRP10"
     polyA_signal    3716..3940
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3986..4414
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4428..4757
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4824..5618
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5792..5913
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5962..5978)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5986..6002
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6010..6040)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6055..6076
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6364..6949)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7120..7980)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7981..8085)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgcc accatggtga gcaagggcga ggagctgttc
      901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1561 atcactctcg gcatggacga gctgtacaag ggaggcggag gtagtggagg agggggaagc
     1621 ggaggcggag ggagtgaatt ctacccatac gatgttccag attacgctgg atccatggcc
     1681 atggccaagg ccagaaagcc ccgggaggca ttgctctggg ccttgagtga ccttgaggag
     1741 aacgatttca agaagttaaa gttctactta cgggatatga ccctgtctga gggccagccc
     1801 ccactggcca gaggggagtt ggagggcctg attccggtgg acctggcaga attactgatt
     1861 tcaaagtatg gagaaaagga ggctgtgaaa gttgtcctca agggcttgaa ggtcatgaac
     1921 ctgttggaac ttgtggacca gctcagccat atttgtctgc atgattacag agaagtatac
     1981 cgagagcatg tgcgctgcct agaggaatgg caggaagcag gagtcaatgg cagatacaac
     2041 caggtgctcc tggtggccaa gcccagctca gagagcccag aatcacttgc ctgccccttc
     2101 ccggagcagg agctggagtc tgtcacggtg gaggctctat ttgattcagg ggaaaagccc
     2161 tcactggccc catccttagt tgtgctacag gggtcggctg gcactggaaa gacaactctc
     2221 gccagaaaaa tggtgttgga ctgggccacc ggtactctgt acccaggccg gtttgattat
     2281 gtcttttatg taagctgcaa agaagtggtc ctgctgctgg agagcaaact ggagcagctc
     2341 cttttctggt gctgcgggga caatcaagcc cctgtcacag agattctgag gcagccagag
     2401 cggctcctgt tcatcctgga tggctttgat gagctgcaga ggccctttga agaaaagttg
     2461 aagaagaggg gtttgagtcc caaggagagc ctgctgcacc ttctaattag gagacataca
     2521 ctccccacgt gctcccttct catcaccacc cggcccctgg ctttgaggaa tctggagccc
     2581 ttgctgaaac aagcacgtca tgtccatatc ctaggcttct ctgaggagga gagggcgagg
     2641 tacttcagct cctatttcac ggatgagaag caagctgacc gtgccttcga cattgtacag
     2701 aaaaatgaca ttctctacaa agcgtgtcag gttccaggca tttgctgggt ggtctgctcc
     2761 tggctgcagg ggcagatgga gagaggcaaa gttgtcttag agacacctag aaacagcact
     2821 gacatcttca tggcttacgt ctccaccttt ctgccgcccg atgatgatgg gggctgctcc
     2881 gagctttccc ggcacagggt cctgaggagt ctgtgctccc tagcagctga agggattcag
     2941 caccagaggt tcctatttga agaagctgag ctcaggaaac ataatttaga tggccccagg
     3001 cttgccgctt tcctgagtag taacgactac caattgggac ttgccatcaa gaagttctac
     3061 agcttccgcc acatcagctt ccaggacttt tttcatgcca tgtcttacct ggtgaaagag
     3121 gaccaaagcc ggctggggaa ggagtcccgc agagaagtgc aaaggctgct ggaggtaaag
     3181 gagcaggaag ggaatgatga gatgaccctc actatgcagt ttttactgga catctcgaaa
     3241 aaagacagct tctcgaactt ggagctcaag ttctgcttca gaatttctcc ctgtttagcg
     3301 caggatctga agcattttaa agaacagatg gaatctatga agcacaacag gacctgggat
     3361 ttggaattct ccctgtatga agctaaaata aagaatctgg taaaaggtat tcagatgaac
     3421 aatgtatcat tcaagataaa acattcaaat gaaaagaaat cacagagcca gaatttattt
     3481 tctgtcaaaa gcagcttgag tcatggacct aaggaggagc aaaaatgtcc ttctgtccat
     3541 ggacagaagg agggcaaaga taatatagca ggaacacaaa aggaagcttc tactggaaaa
     3601 ggcagaggga cagaggaaac accaaaaaat acttacatat aaggatcctg atatccagca
     3661 cagtggcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg
     3721 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     3781 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     3841 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     3901 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     3961 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4021 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4081 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4141 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4201 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4261 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4321 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4381 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     4441 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4501 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4561 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4621 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4681 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4741 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
     4801 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     4861 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     4921 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     4981 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     5041 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     5101 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     5161 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     5221 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     5281 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     5341 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     5401 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     5461 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     5521 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     5581 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     5641 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     5701 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     5761 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     5821 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5881 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     5941 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6001 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6061 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6121 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6181 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6241 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6301 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6361 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6421 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6481 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6541 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6661 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6721 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6781 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     6841 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6901 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6961 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7021 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7081 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7141 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7201 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7261 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7321 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7381 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7441 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7501 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7561 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7621 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7681 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7741 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7801 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7861 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7921 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7981 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8041 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8101 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggt
//
