LOCUS V027960 6592 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V027960 VERSION V027960 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 931..2112 /label="NDRG1(NM_001135242)" /note="NDRG1(NM_001135242)" /gene="NDRG1" polyA_signal 2159..2383 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2429..2857 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2871..3200 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3267..4061 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4235..4356 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4405..4421) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4429..4445 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4453..4483) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4498..4519 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4807..5392) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5563..6423) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6424..6528) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cactagtcca gtgtggtgga attcgccacc atgtctcggg agatgcagga tgtagacctc 961 gctgaggtga agcctttggt ggagaaaggg gagaccatca ccggcctcct gcaagagttt 1021 gatgtccagg agcaggacat cgagacttta catggctctg ttcacgtcac gctgtgtggg 1081 actcccaagg gaaaccggcc tgtcatcctc acctaccatg acatcggcat gaaccacaaa 1141 acctgctaca accccctctt caactacgag gacatgcagg agatcaccca gcactttgcc 1201 gtctgccacg tggacgcccc tggccagcag gacggcgcag cctccttccc cgcagggtac 1261 atgtacccct ccatggatca gctggctgaa atgcttcctg gagtccttca acagtttggg 1321 ctgaaaagca ttattggcat gggaacagga gcaggcgcct acatcctaac tcgatttgct 1381 ctaaacaacc ctgagatggt ggagggcctt gtccttatca acgtgaaccc ttgtgcggaa 1441 ggctggatgg actgggccgc ctccaagatc tcaggatgga cccaagctct gccggacatg 1501 gtggtgtccc acctttttgg gaaggaagaa atgcagagta acgtggaagt ggtccacacc 1561 taccgccagc acattgtgaa tgacatgaac cccggcaacc tgcacctgtt catcaatgcc 1621 tacaacagcc ggcgcgacct ggagattgag cgaccaatgc cgggaaccca cacagtcacc 1681 ctgcagtgcc ctgctctgtt ggtggttggg gacagctcgc ctgcagtgga tgccgtggtg 1741 gagtgcaact caaaattgga cccaacaaag accactctcc tcaagatggc ggactgtggc 1801 ggcctcccgc agatctccca gccggccaag ctcgctgagg ccttcaagta cttcgtgcag 1861 ggcatgggat acatgccctc ggctagcatg acccgcctga tgcggtcccg cacagcctct 1921 ggttccagcg tcacttctct ggatggcacc cgcagccgct cccacaccag cgagggcacc 1981 cgaagccgct cccacaccag cgagggcacc cgcagccgct cgcacaccag cgagggggcc 2041 cacctggaca tcacccccaa ctcgggtgct gctgggaaca gcgccgggcc caagtccatg 2101 gaggtctcct gctagctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact 2161 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 2221 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 2281 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 2341 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga 2401 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg 2461 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 2521 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 2581 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 2641 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 2701 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 2761 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 2821 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt 2881 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 2941 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 3001 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 3061 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 3121 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg 3181 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc 3241 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 3301 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 3361 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 3421 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca 3481 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 3541 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 3601 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 3661 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 3721 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 3781 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 3841 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 3901 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 3961 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 4021 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga 4081 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt 4141 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 4201 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg 4261 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 4321 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc 4381 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 4441 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 4501 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 4561 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 4621 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 4681 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 4741 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 4801 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 4861 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 4921 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 4981 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 5041 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 5101 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 5161 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 5221 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 5281 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 5341 cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 5401 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 5461 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 5521 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 5581 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 5641 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 5701 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 5761 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 5821 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 5881 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 5941 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 6001 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 6061 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 6121 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 6181 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 6241 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 6301 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 6361 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 6421 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 6481 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 6541 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gt //