LOCUS       V043704                 7738 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043704
VERSION     V043704
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             964..3228
                     /label="Myb(NM_001198914)"
                     /note="Myb(NM_001198914)"
                     /gene="Myb"
     CDS             3235..3300
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    3338..3562
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3608..4036
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4050..4379
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4446..5240
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5414..5535
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5584..5600)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5608..5624
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5632..5662)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5677..5698
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5986..6571)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6742..7602)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7603..7707)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc
      961 accatggccc ggagaccccg acacagcatc tacagtagcg atgaagatga tgaagacatt
     1021 gagatgtgtg accatgacta cgatgggctg ctgcccaaat ctggaaagcg tcacttgggg
     1081 aaaactaggt ggacaaggga agaggatgag aagctgaaga agctggtgga acagaacgga
     1141 acagacgact ggaaagtcat tgccaattat ctgcccaacc ggacagatgt gcagtgccaa
     1201 caccggtggc agaaagtgct gaaccctgaa ctcatcaaag gtccctggac caaagaagaa
     1261 gatcagagag tcatagagct tgtccagaaa tatggtccga agcgttggtc tgttattgcc
     1321 aagcacttaa aagggagaat tggaaagcag tgtcgggaga ggtggcacaa ccatttgaat
     1381 ccagaagtta agaaaacctc ctggacagaa gaggaggaca gaatcattta ccaggcacac
     1441 aagcgtctgg ggaacagatg ggcagagatc gcaaagctgc tgcccggacg gactgataat
     1501 gctatcaaga accactggaa ttccaccatg cgtcgcaagg tggaacagga aggctacctg
     1561 caggagcctt ccaaagccag ccagacgcca gtggccacga gcttccagaa gaacaatcat
     1621 ttgatggggt ttgggcatgc ctcacctcca tctcagctct ctccaagtgg ccagtcctcc
     1681 gtcaacagcg aatatcccta ttaccacatc gccgaagcac aaaacatctc cagtcacgtt
     1741 ccctatcctg tcgcattgca tgttaatata gtcaacgtcc ctcagccggc tgcggcagcc
     1801 atccagagac actataacga cgaagaccct gagaaggaaa agcgaataaa ggagctggag
     1861 ttgctcctga tgtcaacaga gaacgagctg aagggacagc aggcattacc aacacagaac
     1921 cacacttgca gctaccccgg gtggcacagc acctccattg tggaccagac cagacctcat
     1981 ggggatagtg cacctgtttc ctgtttggga gaacaccatg ccaccccatc tctgcctgca
     2041 gatcccggct ccctacctga agaaagtgcc tcaccagcaa ggtgcatgat cgtccaccag
     2101 ggcaccattc tggacaatgt taagaacctc ttagaatttg cagaaacact ccagtttata
     2161 gattctgatt cttcgtggtg tgatctcagc agttttgaat tctctgaaga agcggcagct
     2221 ttttcaccta gccagcagcc cacaggcaaa gccttccagc ttcagcaaag agagggccat
     2281 gggactagat ctgcaggaga gcctagcctg agggtgacca ggcgagtgct gagcgaggca
     2341 tccctcggcc cagactcacc ccaagcgagg cacagcaagg ttccgctggt cgtcctacga
     2401 aaaaggcggg gccaggccag ccccctagcc gctggagagc ctagcccctc cctctttgct
     2461 gacgtcatca gctcaactct caagcgttcc cctgtcaaaa gcctaccctt ctctccctcg
     2521 cagttcttga acacttccag caaccatgaa agctcgggct tagatgcacc taccttaccc
     2581 tccactcctc tcattggtca caaactgaca ccatgtcgag accagactgt gaaaacccag
     2641 aaggaaaatt ccatctttag aactccagct atcaaaaggt caatcctcga aagctctcct
     2701 cgaactccca caccattcaa acatgccctt gcagctcaag aaattaaata cggtcccctg
     2761 aagatgctac ctcagacccc ctcccatgca gtggaggacc tacaagatgt gattaagcag
     2821 gaatcggatg aatctggaat tgtggctgag tttcaagaga gtggaccacc gttactgaaa
     2881 aaaatcaagc aggaggtgga gtcgccaact gagaaatcgg gaaacttctt ctgctcaaac
     2941 cactgggcag agaacagcct gagcacccag ctgttctcgc aggcgtctcc tgtggcagat
     3001 gccccaaata ttcttacaag ctctgtttta atgacacctg tatcagaaga tgaagacaat
     3061 gtcctcaaag cctttaccgt acctaagaac aggcccctgg tgggtccctt gcagccatgc
     3121 agtggtgcct gggagccagc atcctgtggg aagacagagg accagatgac ggcctccggt
     3181 ccggctcgga aatacgtgaa cgcgttctca gctcgaactc tggtcatgct cgaggactac
     3241 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag
     3301 tagtgagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     3361 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3421 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3481 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     3541 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     3601 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3661 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     3721 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     3781 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     3841 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     3901 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     3961 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4021 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     4081 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4141 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4201 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4261 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     4321 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     4381 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     4441 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     4501 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     4561 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     4621 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     4681 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     4741 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     4801 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     4861 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     4921 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     4981 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     5041 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     5101 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     5161 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     5221 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     5281 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     5341 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     5401 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5461 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5521 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     5581 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     5641 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     5701 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     5761 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     5821 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5881 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5941 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6001 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6061 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6121 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6181 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6241 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6301 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6361 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6421 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6481 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtttttttg tttgcaagca
     6541 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6601 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6661 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6721 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6781 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6841 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6901 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6961 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7021 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     7081 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     7141 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     7201 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     7261 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7321 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     7381 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7441 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7501 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7561 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7621 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7681 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
