LOCUS       V025712                 9544 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025712
VERSION     V025712
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..4983
                     /label="MST1R(NM_001244937)"
                     /note="MST1R(NM_001244937)"
                     /gene="MST1R"
     CDS             4990..5016
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5020..5046
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5050..5076
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    5111..5335
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      5381..5809
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5823..6152
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6219..7013
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7187..7308
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7357..7373)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7381..7397
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7405..7435)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7450..7471
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7759..8344)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8515..9375)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9376..9480)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggagctcc tcccgccgct gcctcagtcc
      961 ttcctgttgc tgctgctgtt gcctgccaag cccgcggcgg gcgaggactg gcagtgcccg
     1021 cgcaccccct acgcggcctc tcgcgacttt gacgtgaagt acgtggtgcc cagcttctcc
     1081 gccggaggcc tggtacaggc catggtgacc tacgagggcg acagaaatga gagtgctgtg
     1141 tttgtagcca tacgcaatcg cctgcatgtg cttgggcctg acctgaagtc tgtccagagc
     1201 ctggccacgg gccctgctgg agaccctggc tgccagacgt gtgcagcctg tggcccagga
     1261 ccccacggcc ctcccggtga cacagacaca aaggtgctgg tgctggatcc cgcgctgcct
     1321 gcgctggtca gttgtggctc cagcctgcag ggccgctgct tcctgcatga cctagagccc
     1381 caagggacag ccgtgcatct ggcagcgcca gcctgcctct tctcagccca ccataaccgg
     1441 cccgatgact gccccgactg tgtggccagc ccattgggca cccgtgtaac tgtggttgag
     1501 caaggccagg cctcctattt ctacgtggca tcctcactgg acgcagccgt ggctgccagc
     1561 ttcagcccac gctcagtgtc tatcaggcgt ctcaaggctg acgcctcggg attcgcaccg
     1621 ggctttgtgg cgttgtcagt gctgcccaag catcttgtct cctacagtat tgaatacgtg
     1681 cacagcttcc acacgggagc cttcgtatac ttcctgactg tacagccggc cagcgtgaca
     1741 gatgatccta gtgccctgca cacacgcctg gcacggctta gcgccactga gccagagttg
     1801 ggtgactatc gggagctggt cctcgactgc agatttgctc caaaacgcag gcgccggggg
     1861 gccccagaag gcggacagcc ctaccctgtg ctgcgggtgg cccactccgc tccagtgggt
     1921 gcccaacttg ccactgagct gagcatcgcc gagggccagg aagtactatt tggggtcttt
     1981 gtgactggca aggatggtgg tcctggcgtg ggccccaact ctgtcgtctg tgccttcccc
     2041 attgacctgc tggacacact aattgatgag ggtgtggagc gctgttgtga atccccagtc
     2101 catccaggcc tccggcgagg cctcgacttc ttccagtcgc ccagtttttg ccccaacccg
     2161 cctggcctgg aagccctcag ccccaacacc agctgccgcc acttccctct gctggtcagt
     2221 agcagcttct cacgtgtgga cctattcaat gggctgttgg gaccagtaca ggtcactgca
     2281 ttgtatgtga cacgccttga caacgtcaca gtggcacaca tgggcacaat ggatgggcgt
     2341 atcctgcagg tggagctggt caggtcacta aactacttgc tgtatgtgtc caacttctca
     2401 ctgggtgaca gtgggcagcc cgtgcagcgg gatgtcagtc gtcttgggga ccacctactc
     2461 tttgcctctg gggaccaggt tttccaggta cctatccaag gccctggctg ccgccacttc
     2521 ctgacctgtg ggcgttgcct aagggcatgg catttcatgg gctgtggctg gtgtgggaac
     2581 atgtgcggcc agcagaagga gtgtcctggc tcctggcaac aggaccactg cccacctaag
     2641 cttactgagt tccaccccca cagtggacct ctaaggggca gtacaaggct gaccctgtgt
     2701 ggctccaact tctaccttca cccttctggt ctggtgcctg agggaaccca tcaggtcact
     2761 gtgggccaaa gtccctgccg gccactgccc aaggacagct caaaactcag accagtgccc
     2821 cggaaagact ttgtagagga gtttgagtgt gaactggagc ccttgggcac ccaggcagtg
     2881 gggcctacca acgtcagcct caccgtgact aacatgccac cgggcaagca cttccgggta
     2941 gacggcacct ccgtgctgag aggcttctct ttcatggagc cagtgctgat agcagtgcaa
     3001 cccctctttg gcccacgggc aggaggcacc tgtctcactc ttgaaggcca gagtctgtct
     3061 gtaggcacca gccgggctgt gctggtcaat gggactgagt gtctgctagc acgggtcagt
     3121 gaggggcagc ttttatgtgc cacaccccct ggggccacgg tggccagtgt cccccttagc
     3181 ctgcaggtgg ggggtgccca ggtacctggt tcctggacct tccagtacag agaagaccct
     3241 gtcgtgctaa gcatcagccc caactgtggc tacatcaact cccacatcac catctgtggc
     3301 cagcatctaa cttcagcatg gcacttagtg ctgtcattcc atgacgggct tagggcagtg
     3361 gaaagcaggt gtgagaggca gcttccagag cagcagctgt gccgccttcc tgaatatgtg
     3421 gtccgagacc cccagggatg ggtggcaggg aatctgagtg cccgagggga tggagctgct
     3481 ggctttacac tgcctggctt tcgcttccta cccccacccc atccacccag tgccaaccta
     3541 gttccactga agcctgagga gcatgccatt aagtttgagg tctgcgtaga tggtgaatgt
     3601 catatcctgg gtagagtggt gcggccaggg ccagatgggg tcccacagag cacgctcctt
     3661 ggtatcctgc tgcctttgct gctgcttgtg gctgcactgg cgactgcact ggtcttcagc
     3721 tactggtggc ggaggaagca gctagttctt cctcccaacc tgaatgacct ggcatccctg
     3781 gaccagactg ctggagccac acccctgcct attctgtact cgggctctga ctacagaagt
     3841 ggccttgcac tccctgccat tgatggtctg gattccacca cttgtgtcca tggagcatcc
     3901 ttctccgata gtgaagatga atcctgtgtg ccactgctgc ggaaagagtc catccagcta
     3961 agggacctgg actctgcgct cttggctgag gtcaaggatg tgctgattcc ccatgagcgg
     4021 gtggtcaccc acagtgaccg agtcattggc aaaggccact ttggagttgt ctaccacgga
     4081 gaatacatag accaggccca gaatcgaatc caatgtgcca tcaagtcact aagtcgcatc
     4141 acagagatgc agcaggtgga ggccttcctg cgagaggggc tgctcatgcg tggcctgaac
     4201 cacccgaatg tgctggctct cattggtatc atgttgccac ctgagggcct gccccatgtg
     4261 ctgctgccct atatgtgcca cggtgacctg ctccagttca tccgctcacc tcagcggaac
     4321 cccaccgtga aggacctcat cagctttggc ctgcaggtag cccgcggcat ggagtacctg
     4381 gcagagcaga agtttgtgca cagggacctg gctgcgcgga actgcatgct ggacgagtca
     4441 ttcacagtca aggtggctga ctttggtttg gcccgcgaca tcctggacag ggagtactat
     4501 agtgttcaac agcatcgcca cgctcgccta cctgtgaagt ggatggcgct ggagagcctg
     4561 cagacctata gatttaccac caagtctgat gtgtggtcat ttggtgtgct gctgtgggaa
     4621 ctgctgacac ggggtgcccc accataccgc cacattgacc cttttgacct tacccacttc
     4681 ctggcccagg gtcggcgcct gccccagcct gagtattgcc ctgattctct gtaccaagtg
     4741 atgcagcaat gctgggaggc agacccagca gtgcgaccca ccttcagagt actagtgggg
     4801 gaggtggagc agatagtgtc tgcactgctt ggggaccatt atgtgcagct gccagcaacc
     4861 tacatgaact tgggccccag cacctcgcat gagatgaatg tgcgtccaga acagccgcag
     4921 ttctcaccca tgccagggaa tgtacgccgg ccccggccac tctcagagcc tcctcggccc
     4981 actctcgagt acccatacga cgtacctgat tatgcaactt atccttatga tgtacctgat
     5041 tatgctagct atccttatga tgtcccagac tacgcatgag ggcccgttta aacccgctga
     5101 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     5161 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     5221 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     5281 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     5341 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     5401 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5461 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5521 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5581 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5641 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5701 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5761 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     5821 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5881 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     5941 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     6001 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6061 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     6121 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     6181 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     6241 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     6301 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     6361 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     6421 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     6481 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     6541 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     6601 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     6661 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     6721 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     6781 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     6841 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     6901 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     6961 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     7021 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     7081 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     7141 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     7201 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7261 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     7321 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7381 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7441 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7501 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7561 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7621 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7681 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7741 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7801 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7861 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7921 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7981 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8041 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8101 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8161 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     8221 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8281 caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8341 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8401 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8461 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8521 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8581 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8641 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8701 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8761 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8821 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8881 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8941 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9001 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9061 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9121 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9181 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9241 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9301 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9361 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9421 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9481 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     9541 tggt
//
