LOCUS       pcDNA3.1-MME(human)     7637 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038189
VERSION     V038189
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3157
                     /label="MME(NM_000902)"
                     /note="MME(NM_000902)"
                     /gene="MME"
     polyA_signal    3204..3428
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3474..3902
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3916..4245
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4312..5106
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5280..5401
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5450..5466)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5474..5490
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5498..5528)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5543..5564
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5852..6437)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6608..7468)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7469..7573)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg ggcaagtcag aaagtcagat ggatataact gatatcaaca ctccaaagcc
      961 aaagaagaaa cagcgatgga ctccactgga gatcagcctc tcggtccttg tcctgctcct
     1021 caccatcata gctgtgacaa tgatcgcact ctatgcaacc tacgatgatg gtatttgcaa
     1081 gtcatcagac tgcataaaat cagctgctcg actgatccaa aacatggatg ccaccactga
     1141 gccttgtaca gactttttca aatatgcttg cggaggctgg ttgaaacgta atgtcattcc
     1201 cgagaccagc tcccgttacg gcaactttga cattttaaga gatgaactag aagtcgtttt
     1261 gaaagatgtc cttcaagaac ccaaaactga agatatagta gcagtgcaga aagcaaaagc
     1321 attgtacagg tcttgtataa atgaatctgc tattgatagc agaggtggag aacctctact
     1381 caaactgtta ccagacatat atgggtggcc agtagcaaca gaaaactggg agcaaaaata
     1441 tggtgcttct tggacagctg aaaaagctat tgcacaactg aattctaaat atgggaaaaa
     1501 agtccttatt aatttgtttg ttggcactga tgataagaat tctgtgaatc atgtaattca
     1561 tattgaccaa cctcgacttg gcctcccttc tagagattac tatgaatgca ctggaatcta
     1621 taaagaggct tgtacagcat atgtggattt tatgatttct gtggccagat tgattcgtca
     1681 ggaagaaaga ttgcccatcg atgaaaacca gcttgctttg gaaatgaata aagttatgga
     1741 attggaaaaa gaaattgcca atgctacggc taaacctgaa gatcgaaatg atccaatgct
     1801 tctgtataac aagatgacat tggcccagat ccaaaataac ttttcactag agatcaatgg
     1861 gaagccattc agctggttga atttcacaaa tgaaatcatg tcaactgtga atattagtat
     1921 tacaaatgag gaagatgtgg ttgtttatgc tccagaatat ttaaccaaac ttaagcccat
     1981 tcttaccaaa tattctgcca gagatcttca aaatttaatg tcctggagat tcataatgga
     2041 tcttgtaagc agcctcagcc gaacctacaa ggagtccaga aatgctttcc gcaaggccct
     2101 ttatggtaca acctcagaaa cagcaacttg gagacgttgt gcaaactatg tcaatgggaa
     2161 tatggaaaat gctgtgggga ggctttatgt ggaagcagca tttgctggag agagtaaaca
     2221 tgtggtcgag gatttgattg cacagatccg agaagttttt attcagactt tagatgacct
     2281 cacttggatg gatgccgaga caaaaaagag agctgaagaa aaggccttag caattaaaga
     2341 aaggatcggc tatcctgatg acattgtttc aaatgataac aaactgaata atgagtacct
     2401 cgagttgaac tacaaagaag atgaatactt cgagaacata attcaaaatt tgaaattcag
     2461 ccaaagtaaa caactgaaga agctccgaga aaaggtggac aaagatgagt ggataagtgg
     2521 agcagctgta gtcaatgcat tttactcttc aggaagaaat cagatagtct tcccagccgg
     2581 cattctgcag ccccccttct ttagtgccca gcagtccaac tcattgaact atgggggcat
     2641 cggcatggtc ataggacacg aaatcaccca tggcttcgat gacaatggca gaaactttaa
     2701 caaagatgga gacctcgttg actggtggac tcaacagtct gcaagtaact ttaaggagca
     2761 atcccagtgc atggtgtatc agtatggaaa cttttcctgg gacctggcag gtggacagca
     2821 ccttaatgga attaatacac tgggagaaaa cattgctgat aatggaggtc ttggtcaagc
     2881 atacagagcc tatcagaatt atattaaaaa gaatggcgaa gaaaaattac ttcctggact
     2941 tgacctaaat cacaaacaac tatttttctt gaactttgca caggtgtggt gtggaaccta
     3001 taggccagag tatgcggtta actccattaa aacagatgtg cacagtccag gcaatttcag
     3061 gattattggg actttgcaga actctgcaga gttttcagaa gcctttcact gccgcaagaa
     3121 ttcatacatg aatccagaaa agaagtgccg ggtttggtga ctcgagtcta gagggcccgt
     3181 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     3241 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     3301 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     3361 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     3421 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     3481 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3541 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3601 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3661 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     3721 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3781 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     3841 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3901 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     3961 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     4021 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     4081 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4141 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     4201 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     4261 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     4321 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     4381 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     4441 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     4501 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     4561 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     4621 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     4681 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     4741 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     4801 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     4861 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     4921 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     4981 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     5041 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     5101 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     5161 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     5221 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     5281 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5341 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5401 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     5461 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     5521 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     5581 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     5641 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     5701 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5761 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5821 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5881 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5941 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6001 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6061 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6121 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6181 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6241 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6301 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6361 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
     6421 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6481 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6541 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6601 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6661 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6721 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6781 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6841 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6901 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6961 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7021 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7081 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7141 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7201 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7261 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7321 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7381 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7441 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7501 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7561 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     7621 tcccgatccc ctatggt
//
