LOCUS       V042573                 7531 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042573
VERSION     V042573
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             964..3021
                     /label="MASP2(NM_006610)"
                     /note="MASP2(NM_006610)"
                     /gene="MASP2"
     CDS             3028..3093
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    3131..3355
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3401..3829
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3843..4172
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4239..5033
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5207..5328
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5377..5393)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5401..5417
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5425..5455)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5470..5491
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5779..6364)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6535..7395)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7396..7500)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc
      961 accatgaggc tgctgaccct cctgggcctt ctgtgtggct cggtggccac ccccttgggc
     1021 ccgaagtggc ctgaacctgt gttcgggcgc ctggcatccc ccggctttcc aggggagtat
     1081 gccaatgacc aggagcggcg ctggaccctg actgcacccc ccggctaccg cctgcgcctc
     1141 tacttcaccc acttcgacct ggagctctcc cacctctgcg agtacgactt cgtcaagctg
     1201 agctcggggg ccaaggtgct ggccacgctg tgcgggcagg agagcacaga cacggagcgg
     1261 gcccctggca aggacacttt ctactcgctg ggctccagcc tggacattac cttccgctcc
     1321 gactactcca acgagaagcc gttcacgggg ttcgaggcct tctatgcagc cgaggacatt
     1381 gacgagtgcc aggtggcccc gggagaggcg cccacctgcg accaccactg ccacaaccac
     1441 ctgggcggtt tctactgctc ctgccgcgca ggctacgtcc tgcaccgtaa caagcgcacc
     1501 tgctcagccc tgtgctccgg ccaggtcttc acccagaggt ctggggagct cagcagccct
     1561 gaatacccac ggccgtatcc caaactctcc agttgcactt acagcatcag cctggaggag
     1621 gggttcagtg tcattctgga ctttgtggag tccttcgatg tggagacaca ccctgaaacc
     1681 ctgtgtccct acgactttct caagattcaa acagacagag aagaacatgg cccattctgt
     1741 gggaagacat tgccccacag gattgaaaca aaaagcaaca cggtgaccat cacctttgtc
     1801 acagatgaat caggagacca cacaggctgg aagatccact acacgagcac agcgcagcct
     1861 tgcccttatc cgatggcgcc acctaatggc cacgtttcac ctgtgcaagc caaatacatc
     1921 ctgaaagaca gcttctccat cttttgcgag actggctatg agcttctgca aggtcacttg
     1981 cccctgaaat cctttactgc agtttgtcag aaagatggat cttgggaccg gccaatgccc
     2041 gcgtgcagca ttgttgactg tggccctcct gatgatctac ccagtggccg agtggagtac
     2101 atcacaggtc ctggagtgac cacctacaaa gctgtgattc agtacagctg tgaagagacc
     2161 ttctacacaa tgaaagtgaa tgatggtaaa tatgtgtgtg aggctgatgg attctggacg
     2221 agctccaaag gagaaaaatc actcccagtc tgtgagcctg tttgtggact atcagcccgc
     2281 acaacaggag ggcgtatata tggagggcaa aaggcaaaac ctggtgattt tccttggcaa
     2341 gtcctgatat taggtggaac cacagcagca ggtgcacttt tatatgacaa ctgggtccta
     2401 acagctgctc atgccgtcta tgagcaaaaa catgatgcat ccgccctgga cattcgaatg
     2461 ggcaccctga aaagactatc acctcattat acacaagcct ggtctgaagc tgtttttata
     2521 catgaaggtt atactcatga tgctggcttt gacaatgaca tagcactgat taaattgaat
     2581 aacaaagttg taatcaatag caacatcacg cctatttgtc tgccaagaaa agaagctgaa
     2641 tcctttatga ggacagatga cattggaact gcatctggat ggggattaac ccaaaggggt
     2701 tttcttgcta gaaatctaat gtatgtcgac ataccgattg ttgaccatca aaaatgtact
     2761 gctgcatatg aaaagccacc ctatccaagg ggaagtgtaa ctgctaacat gctttgtgct
     2821 ggcttagaaa gtgggggcaa ggacagctgc agaggtgaca gcggaggggc actggtgttt
     2881 ctagatagtg aaacagagag gtggtttgtg ggaggaatag tgtcctgggg ttccatgaat
     2941 tgtggggaag caggtcagta tggagtctac acaaaagtta ttaactatat tccctggatc
     3001 gagaacataa ttagtgattt tctcgaggac tacaaagacc atgacggtga ttataaagat
     3061 catgacatcg actacaagga tgacgatgac aagtagtgag ggcccgttta aacccgctga
     3121 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     3181 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     3241 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     3301 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     3361 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     3421 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3481 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3541 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3601 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3661 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3721 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     3781 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     3841 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3901 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     3961 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     4021 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4081 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     4141 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     4201 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     4261 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     4321 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     4381 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     4441 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     4501 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     4561 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     4621 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     4681 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     4741 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     4801 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     4861 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     4921 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     4981 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     5041 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     5101 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     5161 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     5221 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5281 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     5341 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     5401 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     5461 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     5521 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5581 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5641 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5701 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5761 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     5821 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5881 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5941 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6001 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6061 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6121 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6181 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6241 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6301 caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6361 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     6421 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     6481 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     6541 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     6601 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     6661 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     6721 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     6781 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     6841 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     6901 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     6961 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7021 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7081 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7141 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7201 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7261 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     7321 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     7381 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     7441 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     7501 cacatttccc cgaaaagtgc cacctgacgt c
//
