LOCUS V023824 7547 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V023824 VERSION V023824 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..3037 /label="MASP1(NM_001879)" /note="MASP1(NM_001879)" /gene="MASP1" CDS 3044..3109 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 3147..3371 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3417..3845 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3859..4188 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4255..5049 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5223..5344 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5393..5409) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5417..5433 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5441..5471) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5486..5507 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5795..6380) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6551..7411) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7412..7516) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgaggtggc tgcttctcta 961 ttatgctctg tgcttctccc tgtcaaaggc ttcagcccac accgtggagc taaacaatat 1021 gtttggccag atccagtcgc ctggttatcc agactcctat cccagtgatt cagaggtgac 1081 ttggaatatc actgtcccag atgggtttcg gatcaagctt tacttcatgc acttcaactt 1141 ggaatcctcc tacctttgtg aatatgacta tgtgaaggta gaaactgagg accaggtgct 1201 ggcaaccttc tgtggcaggg agaccacaga cacagagcag actcccggcc aggaggtggt 1261 cctctcccct ggctccttca tgtccatcac tttccggtca gatttctcca atgaggagcg 1321 tttcacaggc tttgatgccc actacatggc tgtggatgtg gacgagtgca aggagaggga 1381 ggacgaggag ctgtcctgtg accactactg ccacaactac attggcggct actactgctc 1441 ctgccgcttc ggctacatcc tccacacaga caacaggacc tgccgagtgg agtgcagtga 1501 caacctcttc actcaaagga ctggggtgat caccagccct gacttcccaa acccttaccc 1561 caagagctct gaatgcctgt ataccatcga gctggaggag ggtttcatgg tcaacctgca 1621 gtttgaggac atatttgaca ttgaggacca tcctgaggtg ccctgcccct atgactacat 1681 caagatcaaa gttggtccaa aagttttggg gcctttctgt ggagagaaag ccccagaacc 1741 catcagcacc cagagccaca gtgtcctgat cctgttccat agtgacaact cgggagagaa 1801 ccggggctgg aggctctcat acagggctgc aggaaatgag tgcccagagc tacagcctcc 1861 tgtccatggg aaaatcgagc cctcccaagc caagtatttc ttcaaagacc aagtgctcgt 1921 cagctgtgac acaggctaca aagtgctgaa ggataatgtg gagatggaca cattccagat 1981 tgagtgtctg aaggatggga cgtggagtaa caagattccc acctgtaaaa ttgtagactg 2041 tagagcccca ggagagctgg aacacgggct gatcaccttc tctacaagga acaacctcac 2101 cacatacaag tctgagatca aatactcctg tcaggagccc tattacaaga tgctcaacaa 2161 taacacaggt atatatacct gttctgccca aggagtctgg atgaataaag tattggggag 2221 aagcctaccc acctgccttc cagtgtgtgg gctccccaag ttctcccgga agctgatggc 2281 caggatcttc aatggacgcc cagcccagaa aggcaccact ccctggattg ccatgctgtc 2341 acacctgaat gggcagccct tctgcggagg ctcccttcta ggctccagct ggatcgtgac 2401 cgccgcacac tgcctccacc agtcactcga tccggaagat ccgaccctac gtgattcaga 2461 cttgctcagc ccttctgact tcaaaatcat cctgggcaag cattggaggc tccggtcaga 2521 tgaaaatgaa cagcatctcg gcgtcaaaca caccactctc cacccccagt atgatcccaa 2581 cacattcgag aatgacgtgg ctctggtgga gctgttggag agcccagtgc tgaatgcctt 2641 cgtgatgccc atctgtctgc ctgagggacc ccagcaggaa ggagccatgg tcatcgtcag 2701 cggctggggg aagcagttct tgcaaaggtt cccagagacc ctgatggaga ttgaaatccc 2761 gattgttgac cacagcacct gccagaaggc ttatgccccg ctgaagaaga aagtgaccag 2821 ggacatgatc tgtgctgggg agaaggaagg gggaaaggac gcctgtgcgg gtgactctgg 2881 aggccccatg gtgaccctga atagagaaag aggccagtgg tacctggtgg gcactgtgtc 2941 ctggggtgat gactgtggga agaaggaccg ctacggagta tactcttaca tccaccacaa 3001 caaggactgg atccagaggg tcaccggagt gaggaacctc gaggactaca aagaccatga 3061 cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt agtgagggcc 3121 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 3181 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 3241 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 3301 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 3361 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc 3421 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 3481 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 3541 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 3601 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 3661 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 3721 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 3781 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 3841 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 3901 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 3961 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 4021 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 4081 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta 4141 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga 4201 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt 4261 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat 4321 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag 4381 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac 4441 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac 4501 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc 4561 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg 4621 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag 4681 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat 4741 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag 4801 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc 4861 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg 4921 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg 4981 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag 5041 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat 5101 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc 5161 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc 5221 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 5281 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 5341 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 5401 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 5461 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 5521 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 5581 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 5641 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 5701 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 5761 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 5821 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 5881 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 5941 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6001 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 6061 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 6121 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 6181 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 6241 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 6301 gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc 6361 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 6421 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 6481 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 6541 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 6601 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 6661 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 6721 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 6781 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 6841 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 6901 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 6961 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 7021 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 7081 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 7141 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 7201 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 7261 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 7321 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 7381 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 7441 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 7501 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc //