LOCUS       V039680                 8837 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039680
VERSION     V039680
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..4357
                     /label="MAN2A2(NM_006122)"
                     /note="MAN2A2(NM_006122)"
                     /gene="MAN2A2"
     polyA_signal    4404..4628
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4674..5102
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5116..5445
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5512..6306
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6480..6601
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6650..6666)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6674..6690
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6698..6728)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6743..6764
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7052..7637)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7808..8668)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8669..8773)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg aagctgaaaa agcaggtgac agtgtgtggg gctgccatct tctgtgtggc
      961 agtcttctcg ctctacctca tgctggaccg agtgcaacac gatcccaccc gacaccagaa
     1021 tggtgggaac ttcccccgga gccaaatttc tgtgctgcag aaccgcattg agcagctgga
     1081 gcagcttttg gaggagaacc atgagattat cagccatatc aaggactccg tgctggagct
     1141 gacagccaac gcagagggcc cgcccgccat gctgccctac tacacggtca atggctcctg
     1201 ggtggtgcca ccggagcccc ggcccagctt cttctccatc tccccgcagg actgccagtt
     1261 tgctttgggg ggccggggtc agaagccaga gctgcagatg ctcactgtgt cggaggagct
     1321 gccgtttgac aacgtggatg gtggtgtgtg gaggcaaggc ttcgacatct cctacgaccc
     1381 gcacgactgg gatgctgaag acctgcaggt gtttgtggtg ccccactctc acaatgaccc
     1441 aggctggatc aagacctttg acaagtacta cacagagcag acccaacaca tcctcaatag
     1501 catggtgtct aagctgcagg aggacccccg gcggcgcttc ctctgggcag aggtctcctt
     1561 cttcgccaag tggtgggaca acatcaatgt ccaaaagaga gcggcagtcc gaaggctggt
     1621 gggaaacggg cagctggaga ttgcgacagg aggctgggtg atgccagatg aggccaattc
     1681 ccactacttt gcattgattg accagctcat cgaaggacac cagtggctgg agagaaatct
     1741 tggtgcaacc ccccgctctg gctgggcagt ggaccccttt ggatacagct ccaccatgcc
     1801 ttacctgctg cgccgtgcca acctcaccag catgctgatt cagagagtgc actatgccat
     1861 caagaagcac tttgctgcca cccacagcct agagttcatg tggaggcaga catgggactc
     1921 ggactccagc acagacatct tctgtcacat gatgcccttc tacagctatg acgtccccca
     1981 tacctgtggc ccagatccca agatctgctg ccaatttgat ttcaaacgcc tgcctggtgg
     2041 gcgcatcaac tgcccttgga aggtgccacc ccgggccatc acagaggcca acgtggcaga
     2101 gagggcagcc ctgcttctgg accaataccg gaagaagtcc cagctgttcc gaagcaacgt
     2161 cctcctggtg cctcttggag atgacttccg atatgacaag ccccaggagt gggatgccca
     2221 gttcttcaac taccaacggc tctttgactt cttcaacagc aggcctaacc tccatgtgca
     2281 ggcccagttt ggcactcttt ctgactattt tgatgccctg tacaagagga caggggtgga
     2341 gccaggggcc cggcctccag ggtttcctgt gctgagcggg gatttcttct cctatgcgga
     2401 ccgggaggat cattactgga caggctatta cacttcccgg cccttctaca agagcttaga
     2461 ccgagtcctg gaagcccacc tgcggggggc agaggttctg tacagcctgg ctgcagctca
     2521 cgctcgccgc tctggtctgg ctggccggta cccactgtct gatttcaccc tcctgacgga
     2581 agctcggcgc acattggggc tcttccagca tcacgatgcc atcactggca cggccaagga
     2641 ggctgtggtg gtggactatg gggtcaggct tctgcgctcc cttgtcaacc tgaagcaggt
     2701 catcattcat gcagcccact atctggtgct gggggacaag gagacctacc actttgaccc
     2761 tgaggcgccc ttcctccaag tggatgacac tcgcttaagt cacgacgccc tcccagagcg
     2821 cacggtgatc cagctggatt cctcgcccag gtttgtggtc ctattcaacc cactggaaca
     2881 ggagcgattc agcatggtgt ccctgctggt caactctccc cgcgtgcgtg tcctttcgga
     2941 ggagggtcag cccctggccg tgcagatcag cgcacactgg agctctgcca ccgaggcggt
     3001 ccctgacgtc taccaggtgt ctgtgcctgt ccgcctgcca gccctgggcc tgggcgtgct
     3061 gcagctacag ctgggcctgg atgggcaccg cacgctgccc tcctctgtgc gcatctacct
     3121 gcacggccgg cagctgtccg tcagcaggca cgaagcgttt cctctccgtg tcattgactc
     3181 tggcaccagc gacttcgccc tcagcaaccg ctacatgcag gtctggttct caggccttac
     3241 tgggctcctc aagagcatcc gaagggtgga tgaggagcac gagcagcagg tggacatgca
     3301 ggtccttgtc tatggcaccc gtacgtccaa agacaagagt ggagcctacc tcttcctgcc
     3361 cgatggcgag gccaagccct acgtccccaa ggagcccccc gtgctgcgtg tcactgaagg
     3421 ccctttcttc tcagaggtgg ttgcgtacta tgagcacatt caccaggcgg tccggcttta
     3481 caatctgcca ggggtggagg ggctgtctct ggacatatca tccctggtgg acatccggga
     3541 ctacgtcaac aaggagctgg ccctgcacat ccatacagac atcgacagcc agggtatctt
     3601 cttcacagac ctcaatggct ttcaggtgca gccccgacgg tatctgaaga agctccccct
     3661 ccaggccaac ttctacccca tgccagtcat ggcctatatc caggacgcac agaagcgcct
     3721 cacgctgcac actgcccagg ccctgggtgt ctctagcctc aaagatggcc agctggaggt
     3781 gatcttggac cggcggctga tgcaggatga caaccggggc ctaggccaag ggctcaagga
     3841 caacaagaga acctgcaacc gtttccgcct cctgctagag cggcgaaccg tgggcagtga
     3901 ggtccaagat agccactcta ccagctaccc atccctcctc agccacctga cctccatgta
     3961 cctgaacgcc ccggcgctcg ctctgcctgt agccaggatg cagctcccag gccctggtct
     4021 gcgctcattt catcctctgg cttcctcact gccctgtgac ttccacctgc tcaacctacg
     4081 tacgctccag gctgaggagg acaccctacc ctcggcggag accgcactca tcttacaccg
     4141 caagggtttt gactgcggcc tggaggccaa gaacttgggc ttcaactgca ccacaagcca
     4201 aggcaaggta gccctgggca gccttttcca tggcctggat gtggtattcc ttcagccaac
     4261 ctccttgacg ttactgtacc ctctggcctc cccgtccaac agcactgacg tctatttgga
     4321 gcccatggag attgctacct ttcgcctccg cttgggttag ctcgagtcta gagggcccgt
     4381 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     4441 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     4501 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     4561 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     4621 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     4681 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4741 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4801 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4861 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4921 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4981 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5041 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5101 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     5161 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     5221 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     5281 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     5341 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     5401 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     5461 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     5521 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     5581 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     5641 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     5701 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     5761 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     5821 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     5881 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     5941 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     6001 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     6061 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     6121 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     6181 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     6241 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     6301 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     6361 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     6421 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     6481 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6541 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6601 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     6661 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6721 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6781 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6841 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6901 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6961 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7021 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7081 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7141 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7201 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7261 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7321 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7381 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7441 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7501 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7561 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
     7621 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7681 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7741 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7801 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7861 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7921 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7981 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8041 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8101 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8161 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8221 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8281 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8341 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8401 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8461 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8521 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8581 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8641 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8701 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8761 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     8821 tcccgatccc ctatggt
//
