LOCUS       V019396                 8294 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019396
VERSION     V019396
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3784
                     /label="LONP1(NM_004793)"
                     /note="LONP1(NM_004793)"
                     /gene="LONP1"
     CDS             3785..3814
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     polyA_signal    3861..4085
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4131..4559
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4573..4902
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4969..5763
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5937..6058
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6107..6123)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6131..6147
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6155..6185)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6200..6221
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6509..7094)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7265..8125)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8126..8230)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gcggcgagca ctggctacgt gcgactgtgg ggagcggcgc ggtgctgggt
      961 gctgcggcgg ccgatgctgg ccgccgccgg ggggcgggtt cccactgcag caggagcgtg
     1021 gttgctccga ggccagcgga cctgcgacgc ctctcctcct tgggcactgt ggggccgagg
     1081 cccggcaatt gggggccaat ggcgggggtt ttgggaagcg agcagccgcg gcggaggcgc
     1141 attctcgggg ggcgaggacg cctccgaggg cggcgcggag gaaggagccg gcggcgcggg
     1201 gggcagcgcg ggcgccgggg aaggcccggt cataacggcg ctcacgccca tgacgatccc
     1261 cgatgtgttt ccgcacctgc cgctcatcgc catcacccgc aacccggtgt tcccgcgctt
     1321 tatcaagatt atcgaggttc aaaataagaa gttggttgag ctgctgagaa ggaaagttcg
     1381 tctcgcccag ccttatgtcg gcgtctttct aaagagagat gacagcaatg agtcggatgt
     1441 ggtcgagagc ctggatgaaa tctaccacac ggggacgttt gcccagatcc atgagatgca
     1501 ggaccttggg gacaagctgc gcatgatcgt catgggacac agaagagtcc atatcagcag
     1561 acagctggag gtggagcccg aggagccgga ggcggagaac aagcacaagc cccgcaggaa
     1621 gtcaaagcgg ggcaagaagg aggcggagga cgagctgagc gccaggcacc cggcggagct
     1681 ggcgatggag cccacccctg agctcccggc tgaggtgctc atggtggagg tagagaacgt
     1741 tgtccacgag gacttccagg tcacggagga ggtgaaagcc ctgactgcag agatcgtgaa
     1801 gaccatccgg gacatcattg ccttgaaccc tctctacagg gagtcagtgc tgcagatgat
     1861 gcaggctggc cagcgggtgg tggacaaccc catctacctg agcgacatgg gcgccgcgct
     1921 caccggggcc gagtcccatg agctgcagga cgtcctggaa gagaccaata ttcctaagcg
     1981 gctgtacaag gccctctccc tgctgaagaa ggaatttgaa ctgagcaagc tgcagcagcg
     2041 cctggggcgg gaggtggagg agaagatcaa gcagacccac cgtaagtacc tgctgcagga
     2101 gcagctaaag atcatcaaga aggagctggg cctggagaag gacgacaagg atgccatcga
     2161 ggagaagttc cgggagcgcc tgaaggagct cgtggtcccc aagcacgtca tggatgttgt
     2221 ggacgaggag ctgagcaagc tgggcctgct ggacaaccac tcctcggagt tcaatgtcac
     2281 ccgcaactac ctagactggc tcacgtccat cccttggggc aagtacagca acgagaacct
     2341 ggacctggcg cgggcacagg cagtgctgga ggaagaccac tacggcatgg aggacgtcaa
     2401 gaaacgcatc ctggagttca ttgccgttag ccagctccgc ggctccaccc agggcaagat
     2461 cctctgcttc tatggccccc ctggcgtggg taagaccagc attgctcgct ccatcgcccg
     2521 cgccctgaac cgagagtact tccgcttcag cgtcgggggc atgactgacg tggctgagat
     2581 caagggccac aggcggacct acgtgggcgc catgcccggg aagatcatcc agtgtttgaa
     2641 gaagaccaag acggagaacc ccctgatcct catcgacgag gtggacaaga tcggccgagg
     2701 ctaccagggg gacccgtcgt cggcactgct ggagctgctg gacccagagc agaatgccaa
     2761 cttcctggac cactacctgg acgtgcccgt ggacttgtcc aaggtgctgt tcatctgcac
     2821 ggccaacgtc acggacacca tccccgagcc gctgcgagac cgtatggaga tgatcaacgt
     2881 gtcgggctac gtggcccagg agaagctggc cattgcggag cgctacctgg tgccccaggc
     2941 tcgcgccctg tgtggcttgg atgagagcaa ggccaagctg tcatcggacg tgctgacgct
     3001 gctcatcaag cagtactgcc gcgagagcgg tgtccgcaac ctgcagaagc aagtggagaa
     3061 ggtgttacgg aaatcggcct acaagattgt cagcggcgag gccgagtccg tggaggtgac
     3121 gcccgagaac ctgcaggact tcgtggggaa gcccgtgttc accgtggagc gcatgtatga
     3181 cgtgacaccg cccggcgtgg tcatggggct ggcctggacc gcaatgggag gctccacgct
     3241 gtttgtggag acatccctga gacggccaca ggacaaggat gccaagggtg acaaggatgg
     3301 cagcctggag gtgacaggcc agctggggga ggtgatgaag gagagcgccc gcatagccta
     3361 caccttcgcc agagccttcc tcatgcagca cgcccccgcc aatgactacc tggtgacctc
     3421 acacatccac ctgcatgtgc ccgagggcgc cacccccaag gacggcccaa gcgcaggctg
     3481 caccatcgtc acggccctgc tgtccctggc catgggcagg cctgtccggc agaatctggc
     3541 catgactggc gaagtctccc tcacgggcaa gatcctgcct gttggtggca tcaaggagaa
     3601 gaccattgcg gccaagcgcg caggggtgac gtgcatcgtc ctgccagccg agaacaagaa
     3661 ggacttctac gacctggcag ccttcatcac cgagggcctg gaggtgcact tcgtggaaca
     3721 ctaccgggag atcttcgaca tcgccttccc ggacgagcag gcagaggcgc tggccgtgga
     3781 acgggagcaa aagctcattt ctgaagagga cttgtgactc gagtctagag ggcccgttta
     3841 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     3901 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     3961 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4021 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4081 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     4141 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4201 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4261 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4321 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4381 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4441 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     4501 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     4561 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     4621 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     4681 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     4741 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     4801 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     4861 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     4921 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     4981 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     5041 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     5101 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     5161 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     5221 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     5281 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     5341 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     5401 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     5461 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     5521 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     5581 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     5641 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     5701 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     5761 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     5821 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     5881 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     5941 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6001 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6061 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6121 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6181 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6241 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6301 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6361 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6421 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6481 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6541 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6601 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6661 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6721 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6781 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6841 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6901 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     6961 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7021 gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7081 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7141 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7201 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7261 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7321 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7381 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7441 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7501 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7561 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7621 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7681 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7741 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7801 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7861 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7921 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7981 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8041 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8101 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8161 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8221 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     8281 cgatccccta tggt
//
