LOCUS V023682 6311 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V023682 VERSION V023682 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..1801 /label="LAIR1(NM_002287)" /note="LAIR1(NM_002287)" /gene="LAIR1" CDS 1808..1873 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 1911..2135 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2181..2609 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2623..2952 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3019..3813 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3987..4108 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4157..4173) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4181..4197 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4205..4235) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4250..4271 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4559..5144) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5315..6175) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6176..6280) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgtctcccc accccaccgc 961 cctcctgggc ctagtgctct gcctggccca gaccatccac acgcaggagg aagatctgcc 1021 cagaccctcc atctcggctg agccaggcac cgtgatcccc ctggggagcc atgtgacttt 1081 cgtgtgccgg ggcccggttg gggttcaaac attccgcctg gagagggaca gtagatccac 1141 atacaatgat actgaagatg tgtctcaagc tagtccatct gagtcagagg ccagattccg 1201 cattgactca gtaagagaag gaaatgccgg gctttatcgc tgcatctatt ataagccccc 1261 taaatggtct gagcagagtg actacctgga gctgctggtg aaagaaagct ctggaggccc 1321 ggactccccg gacacagagc ccggctcctc agctggaccc acgcagaggc cgtcggacaa 1381 cagtcacaat gagcatgcac ctgcttccca aggcctgaaa gctgagcatc tgtatattct 1441 catcggggtc tcagtggtct tcctcttctg tctcctcctc ctggtcctct tctgcctcca 1501 tcgccagaat cagataaagc aggggccccc cagaagcaag gacgaggagc agaagccaca 1561 gcagaggcct gacctggctg ttgatgttct agagaggaca gcagacaagg ccacagtcaa 1621 tggacttcct gagaaggaca gagagacgga cacctcggcc ctggctgcag ggagttccca 1681 ggaggtgacg tatgctcagc tggaccactg ggccctcaca cagaggacag cccgggctgt 1741 gtccccacag tccacaaagc ccatggccga gtccatcacg tatgcagccg ttgccagaca 1801 cctcgaggac tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga 1861 tgacgatgac aagtagtgag ggcccgttta aacccgctga tcagcctcga ctgtgccttc 1921 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 1981 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 2041 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 2101 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg 2161 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 2221 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 2281 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 2341 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 2401 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 2461 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 2521 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 2581 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt 2641 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 2701 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 2761 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 2821 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 2881 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 2941 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca 3001 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct 3061 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc 3121 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc 3181 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc 3241 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg 3301 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc 3361 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac 3421 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat 3481 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc 3541 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg 3601 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg 3661 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc 3721 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc 3781 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg 3841 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa 3901 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc 3961 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat 4021 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 4081 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga 4141 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 4201 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 4261 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 4321 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 4381 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 4441 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 4501 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 4561 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 4621 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 4681 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 4741 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 4801 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 4861 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 4921 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 4981 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 5041 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt 5101 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 5161 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 5221 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 5281 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 5341 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 5401 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 5461 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 5521 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 5581 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 5641 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 5701 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 5761 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 5821 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 5881 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 5941 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 6001 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 6061 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 6121 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 6181 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 6241 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 6301 cacctgacgt c //