LOCUS       V032626                11051 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032626
VERSION     V032626
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             884..1591
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1628..6571
                     /label="KIF14(NM_014875)"
                     /note="KIF14(NM_014875)"
                     /gene="KIF14"
     polyA_signal    6618..6842
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      6888..7316
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7330..7659
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7726..8520
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8694..8815
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8864..8880)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8888..8904
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8912..8942)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8957..8978
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9266..9851)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10022..10882)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10883..10987)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgct accggtcgcc accatggtga gcaagggcga
      901 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      961 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     1021 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     1081 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     1141 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     1201 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     1261 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     1321 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     1381 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     1441 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     1501 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     1561 ctccaccggc ggcatggacg agctgtacaa gggtggaggc ggatcaggag gcggtggatc
     1621 tctcgagatg tcattacaca gtactcataa tagaaataac agcggtgata ttcttgatat
     1681 tccttcttcc caaaatagtt catcactgaa tgccctcacc cacagtagcc gacttaagct
     1741 gcatttgaag tcggatatgt cagaatgtga aaatgatgat ccattattga gatctgcagg
     1801 taaagtcaga gacataaata gaacttatgt tatttctgcc agtagaaaaa cagcagacat
     1861 gccccttacc cctaatcctg taggtagatt ggcacttcag aggagaacta caaggaacaa
     1921 agaatcatct ttgcttgtta gtgagttgga agacacaact gaaaaaacag cagaaacacg
     1981 tcttacatta caacgtcgtg ctaaaacaga ttctgcagaa aagtggaaaa cagctgaaat
     2041 agattctgtc aaaatgacac tgaatgtggg aggtgaaaca gaaaataatg gtgtttctaa
     2101 ggaaagtaga acaaatgtaa ggattgtaaa taatgctaaa aactcttttg ttgcctcttc
     2161 tgtaccttta gatgaagatc cacaggtcat tgaaatgatg gctgataaga aatacaaaga
     2221 aacattttct gcccccagta gagcaaatga aaatgttgca cttaagtact caagtaatag
     2281 accacccatt gcttccctga gtcagactga agttgttaga tcaggacact tgacaacgaa
     2341 acctactcag agcaagttgg atatcaaagt gttgggaaca ggaaacttgt atcatagaag
     2401 tattgggaag gaaattgcaa aaacttcaaa taaatttggg agcttagaaa aaagaacacc
     2461 tacaaaatgt acaacagaac acaaactgac aacaaagtgc agcctgcctc agcttaagag
     2521 cccagctcca tcaatactga agaatagaat gtctaacctt caagttaaac aaagaccaaa
     2581 aagttccttt cttgcaaata aacaggaaag atccgcagaa aatacaattc ttcccgaaga
     2641 agaaactgta gttcagaaca cctctgcagg aaaagacccc ttaaaagtag agaatagtca
     2701 agtgacagtg gcagtacgcg taagaccttt caccaagaga gagaagattg aaaaagcatc
     2761 ccaggtagtc ttcatgagtg ggaaagaaat aactgtggaa caccctgaca cgaaacaagt
     2821 ttataatttt atttatgatg tttcattctg gtcttttgat gaatgtcatc ctcactacgc
     2881 tagccagaca actgtctatg agaagctagc agcaccactc ctagaaagag ccttcgaagg
     2941 cttcaatacc tgtctttttg cttatggtca gactggctct ggaaaatcat atacgatgat
     3001 gggatttagt gaagaaccag gaataattcc aagattttgt gaagatcttt tttctcaagt
     3061 agccagaaaa caaacccaag aggtcagcta tcacattgaa atgagcttct ttgaagtata
     3121 taatgaaaaa attcacgacc ttctggtttg taaagatgaa aatgggcaga gaaagcaacc
     3181 actgagagtg agggaacatc ctgtttatgg accatatgtt gaagcactgt caatgaacat
     3241 tgtcagttct tacgctgata tccagagttg gctagaattg ggaaataaac aaagagctac
     3301 tgctgctact ggtatgaatg ataaaagttc ccgatctcat tcagttttca ccctggtgat
     3361 gacccagacc aagacagaat ttgtggaagg ggaagaacac gatcacagaa taacaagtcg
     3421 aattaaccta atagatctgg caggcagtga gcgctgctct acggctcaca ctaatggaga
     3481 tcgactaaag gaaggtgtga gtattaataa gtccttgcta actttgggaa aagttatatc
     3541 tgcactttcg gaacaagcaa accaaaggag tgtttttatt ccttatcgtg aatctgttct
     3601 tacatggctg ttaaaagaaa gtctgggtgg aaattcaaaa actgcaatga ttgctacgat
     3661 tagtcccgct gccagcaaca tagaagaaac attaagcaca cttagatatg ctaaccaagc
     3721 ccgtttaata gtcaacattg ctaaagtaaa tgaagatatg aacgctaagt taattagaga
     3781 attgaaggca gaaattgcaa agctaaaagc tgctcagaga aacagtcgga atattgaccc
     3841 tgaacgatac aggctctgtc ggcaagaaat aacatcctta agaatgaaac tgcatcaaca
     3901 ggagagagac atggcagaaa tgcaaagagt gtggaaagaa aagtttgaac aagctgaaaa
     3961 aagaaaactt caagaaacaa aagagttaca gaaagcagga attatgtttc aaatggacaa
     4021 tcatttacca aaccttgtta atctgaatga agatccacaa ctatctgaga tgctgctata
     4081 tatgataaaa gaaggaacaa ctacagttgg aaagtataaa ccaaactcaa gccatgatat
     4141 tcagttatct ggggtgctga ttgctgatga tcattgtact atcaaaaatt ttggtgggac
     4201 agtgagtatt atcccagttg gggaagcaaa gacatatgta aatggaaaac atattttgga
     4261 aatcacagta ttacgtcatg gtgatcgagt gattcttggt ggagatcatt attttagatt
     4321 taatcatcca gtagaagtcc agaaaggaaa aaggccatct ggaagagata ctcctataag
     4381 tgagggtcca aaagactttg aatttgcaaa aaatgagttg ctcatggcac agagatcaca
     4441 acttgaagca gaaataaaag aggctcagtt gaaggcaaag gaagaaatga tgcaaggaat
     4501 ccagattgca aaagaaatgg ctcagcaaga gctttcttct caaaaagctg catatgaaag
     4561 caaaataaaa gcactggaag cagaactgag agaagagtct caaaggaaaa aaatgcagga
     4621 aataaataac cagaaggcta atcacaaaat tgaggaatta gaaaaggcaa agcagcatct
     4681 tgaacaggaa atatatgtca acaaaaagcg attagaaatg gaaacattgg ctacaaaaca
     4741 ggctttagaa gaccatagca tccgccatgc aagaattctg gaagctttag aaactgaaaa
     4801 gcaaaaaatt gctaaagaag tacaaattct acagcagaat cggaataata gggataaaac
     4861 ttttacagtg cagacaactt ggagctctat gaaactctca atgatgattc aggaagccaa
     4921 tgctatcagc agcaaattga aaacatacta tgtttttggc agacatgata tatcagataa
     4981 aagtagttct gacacttcta ttcgggttcg taacctgaaa ctaggaatct caacattctg
     5041 gagtctggaa aagtttgaat ctaaacttgc agcaatgaaa gaactttatg agagtaatgg
     5101 tagtaacagg ggtgaagatg ccttttgtga tcctgaagat gaatgggaac ccgacattac
     5161 agatgcacca gtttcttcac tttctagaag gaggagtagg agtttgatga agaacagaag
     5221 aatttctggt tgtttacatg acatacaagt ccatccaatt aagaatttgc attcttcaca
     5281 ttcatcaggt ttaatggaca aatcaagcac tatttactca aattcagcag agtcctttct
     5341 tcctggaatt tgcaaagaat tgattggttc ttcgttagat ttttttggac agagttatga
     5401 tgaagaaaga actatagcag acagcctaat taatagtttt cttaaaattt ataatgggct
     5461 atttgccatt tccaaggctc atgaagaaca agatgaagaa agtcaagata acttgttttc
     5521 ttctgatcga gcaatccagt cacttactat tcagactgca tgtgcttttg agcagctagt
     5581 agtgctaatg aaacactggc tgagtgattt actgccttgt accaacatag caagacttga
     5641 ggatgagttg agacaagaag ttaaaaaact gggaggctac ttacagttat ttttgcaggg
     5701 atgctgtttg gatatttcat caatgataaa agaggctcaa aagaatgcaa tccaaattgt
     5761 acaacaagct gtaaagtatg tggggcagtt agcagttctg aaagggagca agctacattt
     5821 tctagaaaac ggtaacaata aagctgccag tgtccaggag gaattcatgg atgctgtttg
     5881 tgatggtgta ggcttaggaa tgaagatttt attagattct ggactggaaa aagcaaaaga
     5941 acttcagcat gaactcttta ggcagtgtac aaaaaatgag gttaccaaag aaatgaaaac
     6001 taatgccatg ggattgatta gatctcttga aaacatcttt gctgaatcga aaattaaaag
     6061 tttcagaagg caagtacaag aagaaaactt tgaataccaa gatttcaaga ggatggttaa
     6121 tcgtgctcca gaattcttaa agttaaaaca ttgcttagag aaagctattg aaattattat
     6181 ttctgcactg aaaggatgcc atagtgatat aaatcttctc cagacttgtg ttgaaagtat
     6241 tcgcaacttg gccagtgatt tttacagtga cttcagtgtg ccttctactt ctgttggcag
     6301 ctatgagagt agagtaactc acattgtcca ccaggaacta gaatctctag ctaagtctct
     6361 cctcttttgt tttgaatctg aagaaagccc tgatttgttg aaaccctggg aaacttataa
     6421 tcaaaatacc aaagaagaac accaacaatc taaatcaagc gggattgacg gcagtaagaa
     6481 taaaggtgta ccaaagcgtg tctatgagct ccatggctca tccccagcag tgagctcaga
     6541 ggaatgcaca cccagtagga ttcagtgggt gtgaggatcc tctagagggc ccgtttaaac
     6601 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     6661 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     6721 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     6781 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     6841 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     6901 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6961 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     7021 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     7081 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     7141 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     7201 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     7261 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     7321 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     7381 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     7441 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     7501 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7561 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     7621 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     7681 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     7741 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     7801 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     7861 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     7921 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     7981 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     8041 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     8101 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     8161 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     8221 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     8281 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     8341 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     8401 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     8461 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     8521 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     8581 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     8641 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     8701 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8761 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8821 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     8881 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8941 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     9001 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9061 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9121 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9181 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9241 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9301 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9361 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9421 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9481 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9541 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9601 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9661 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9721 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9781 aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9841 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9901 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9961 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    10021 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    10081 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    10141 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    10201 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    10261 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    10321 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    10381 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    10441 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    10501 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    10561 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10621 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    10681 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    10741 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10801 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10861 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10921 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10981 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
    11041 tcccctatgg t
//
