LOCUS V045686 7974 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V045686 VERSION V045686 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 890..2764 /label="KARS1(NM_001130089)" /note="KARS1(NM_001130089)" /gene="KARS1" CDS 2834..3490 /codon_start=1 /gene="" /product="glutathione S-transferase from Schistosoma japonicum" /transl_table=1 /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK*" /label="GST" polyA_signal 3541..3765 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3811..4239 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4253..4582 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4649..5443 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5617..5738 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5787..5803) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5811..5827 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5835..5865) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5880..5901 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6189..6774) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6945..7805) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7806..7910) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttgccacca tgttgacgca 901 agctgctgta aggcttgtta gggggtccct gcgcaaaacc tcctgggcag agtggggtca 961 cagggaactg cgactgggtc aacttgctcc tttcacagcg cctcacaagg acaagtcatt 1021 ttctgatcaa agaagtgagc tgaagagacg cctgaaagct gagaagaaag tagcagagaa 1081 ggaggccaaa cagaaagagc tcagtgagaa acagctaagc caagccactg ctgctgccac 1141 caaccacacc actgataatg gtgtgggtcc tgaggaagag agcgtggacc caaatcaata 1201 ctacaaaatc cgcagtcaag caattcatca gctgaaggtc aatggggaag acccataccc 1261 acacaagttc catgtagaca tctcactcac tgacttcatc caaaaatata gtcacctgca 1321 gcctggggat cacctgactg acatcacctt aaaggtggca ggtaggatcc atgccaaaag 1381 agcttctggg ggaaagctca tcttctatga tcttcgagga gagggggtga agttgcaagt 1441 catggccaat tccagaaatt ataaatcaga agaagaattt attcatatta ataacaaact 1501 gcgtcgggga gacataattg gagttcaggg gaatcctggt aaaaccaaga agggtgagct 1561 gagcatcatt ccgtatgaga tcacactgct gtctccctgt ttgcatatgt tacctcatct 1621 tcactttggc ctcaaagaca aggaaacaag gtatcgccag agatacttgg acttgatcct 1681 gaatgacttt gtgaggcaga aatttatcat ccgctctaag atcatcacat atataagaag 1741 tttcttagat gagctgggat tcctagagat tgaaactccc atgatgaaca tcatcccagg 1801 gggagccgtg gccaagcctt tcatcactta tcacaacgag ctggacatga acttatatat 1861 gagaattgct ccagaactct atcataagat gcttgtggtt ggtggcatcg accgggttta 1921 tgaaattgga cgccagttcc ggaatgaggg gattgatttg acgcacaatc ctgagttcac 1981 cacctgtgag ttctacatgg cctatgcaga ctatcacgat ctcatggaaa tcacggagaa 2041 gatggtttca gggatggtga agcatattac aggcagttac aaggtcacct accacccaga 2101 tggcccagag ggccaagcct acgatgttga cttcacccca cccttccggc gaatcaacat 2161 ggtagaagag cttgagaaag ccctggggat gaagctgcca gaaacgaacc tctttgaaac 2221 tgaagaaact cgcaaaattc ttgatgatat ctgtgtggca aaagctgttg aatgccctcc 2281 acctcggacc acagccaggc tccttgacaa gcttgttggg gagttcctgg aagtgacttg 2341 catcaatcct acattcatct gtgatcaccc acagataatg agccctttgg ctaaatggca 2401 ccgctctaaa gagggtctga ctgagcgctt tgagctgttt gtcatgaaga aagagatatg 2461 caatgcgtat actgagctga atgatcccat gcggcagcgg cagctttttg aagaacaggc 2521 caaggccaag gctgcaggtg atgatgaggc catgttcata gatgaaaact tctgtactgc 2581 cctggaatat gggctgcccc ccacagctgg ctggggcatg ggcattgatc gagtcgccat 2641 gtttctcacg gactccaaca acatcaagga agtacttctg tttcctgcca tgaaacccga 2701 agacaagaag gagaatgtag caaccactga tacactggaa agcacaacag ttggcacttc 2761 tgtcggatcc ggcggcggag ggagtggagg agggggaagc ggaggcggag ggagtaagaa 2821 ttctgcagat atcatgtccc ctatactagg ttattggaaa attaagggcc ttgtgcaacc 2881 cactcgactt cttttggaat atcttgaaga aaaatatgaa gagcatttgt atgagcgcga 2941 tgaaggtgat aaatggcgaa acaaaaagtt tgaattgggt ttggagtttc ccaatcttcc 3001 ttattatatt gatggtgatg ttaaattaac acagtctatg gccatcatac gttatatagc 3061 tgacaagcac aacatgttgg gtggttgtcc aaaagagcgt gcagagattt caatgcttga 3121 aggagcggtt ttggatatta gatacggtgt ttcgagaatt gcatatagta aagactttga 3181 aactctcaaa gttgattttc ttagcaagct acctgaaatg ctgaaaatgt tcgaagatcg 3241 tttatgtcat aaaacatatt taaatggtga tcatgtaacc catcctgact tcatgttgta 3301 tgacgctctt gatgttgttt tatacatgga cccaatgtgc ctggatgcgt tcccaaaatt 3361 agtttgtttt aaaaaacgta ttgaagctat cccacaaatt gataagtact tgaaatccag 3421 caagtatata gcatggcctt tgcagggctg gcaagccacg tttggtggtg gcgaccatcc 3481 tccaaaatga gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga 3541 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc 3601 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc 3661 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt 3721 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa 3781 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg 3841 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 3901 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 3961 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 4021 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 4081 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 4141 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 4201 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca 4261 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 4321 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 4381 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 4441 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 4501 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt 4561 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga 4621 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 4681 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 4741 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 4801 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 4861 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 4921 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 4981 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 5041 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 5101 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 5161 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 5221 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 5281 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 5341 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 5401 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc 5461 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc 5521 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag 5581 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat 5641 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 5701 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc 5761 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 5821 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 5881 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 5941 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 6001 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 6061 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 6121 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 6181 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 6241 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 6301 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 6361 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 6421 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 6481 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 6541 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 6601 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 6661 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 6721 agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 6781 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 6841 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 6901 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 6961 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 7021 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 7081 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 7141 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 7201 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 7261 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 7321 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 7381 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 7441 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 7501 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 7561 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 7621 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 7681 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 7741 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 7801 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 7861 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 7921 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggt //