LOCUS       V046492                13449 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046492
VERSION     V046492
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             893..919
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             926..8938
                     /label="ITPR3(NM_002224)"
                     /note="ITPR3(NM_002224)"
                     /gene="ITPR3"
     polyA_signal    9016..9240
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      9286..9714
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9728..10057
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             10124..10918
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    11092..11213
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(11262..11278)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11286..11302
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11310..11340)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11355..11376
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11664..12249)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12420..13280)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13281..13385)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttgccacca tgtaccctta
      901 tgacgtcccc gattacgccg gtaccatgag tgaaatgtcc agctttcttc acatcgggga
      961 catcgtctcc ctgtacgccg agggctccgt caatggcttc atcagcactt tggggctggt
     1021 ggatgaccgc tgtgtggtgg agcccgcggc cggggacctg gacaaccccc ctaagaagtt
     1081 ccgtgactgc ctcttcaagg tgtgccccat gaaccgctac tcggcccaga agcagtactg
     1141 gaaggccaag cagactaagc aggacaagga gaagatcgct gatgtggtgt tgctgcagaa
     1201 gctgcagcat gcggcgcaga tggagcagaa gcaaaatgac acggagaaca agaaggtgca
     1261 tggggatgtc gtgaagtatg gcagtgtgat ccagctcctg cacatgaaga gcaacaagta
     1321 cctgacagtg aacaagcggc ttccggcctt gctggagaag aacgccatgc gggtgactct
     1381 ggatgccaca ggcaacgagg gttcctggct cttcatccag cccttctgga agctgcggag
     1441 caacggggac aacgtggtcg tgggggacaa ggtgatcctg aatcctgtca atgccgggca
     1501 gcctctgcat gccagcaatt acgagctcag cgacaacgcc ggctgcaagg aggtcaattc
     1561 tgtgaactgc aacaccagct ggaagatcaa cctgtttatg cagtttcggg accacctgga
     1621 ggaggtgttg aaagggggag acgtggtgcg gctgttccat gcggagcagg agaagttcct
     1681 gacgtgtgac gagtacaagg gcaagctgca ggtgttcctg cgaactacac tgcgccagtc
     1741 tgccacctcg gccaccagct ccaatgctct ctgggaggtg gaggtggtcc accacgaccc
     1801 ctgccgtgga ggagctgggc actggaatgg cttgtaccgc ttcaagcacc tggctacagg
     1861 caactacctg gctgctgagg agaaccccag ttacaaaggt gatgcctcag atcccaaggc
     1921 agcaggaatg ggggcacagg gccgcacagg ccgcaggaat gctggggaga agatcaagta
     1981 ctgcctggtg gctgtgcctc atggcaatga catcgcctct ctctttgagc tggaccccac
     2041 caccttgcag aaaaccgact ctttcgtgcc ccggaactcg tacgtccggc tgcggcacct
     2101 ctgcaccaac acgtggattc agagcaccaa tgtgcccatt gacatcgagg aggagcggcc
     2161 catccggctc atgctgggca cctgccccac caaggaggac aaggaggcct ttgccatcgt
     2221 gtcagtgccc gtgtctgaga tccgagacct ggactttgcc aatgacgcca gctccatgct
     2281 ggccagtgcc gtggagaaac tcaacgaggg cttcatcagc cagaatgacc gcaggtttgt
     2341 catccagctg ctggaagacc tggtgttctt tgtcagcgat gtccccaaca atgggcagaa
     2401 tgtcctggac atcatggtca ctaagcccaa ccgggaacgg cagaagctga tgagggagca
     2461 gaacatcctc aaacaggtct ttggcattct gaaggccccg ttccgtgaga aggggggtga
     2521 aggtcccctg gtgcggctgg aggagctgtc agaccagaag aacgccccct accagcacat
     2581 gttccgcctg tgctaccgcg tgttgcggca ttcccaggag gactaccgca agaaccagga
     2641 gcacattgcc aagcagtttg ggatgatgca gtcccagatt ggctacgaca tcctggccga
     2701 ggacaccatc actgccctgc tgcacaacaa ccgcaagctc ctggaaaagc acatcaccaa
     2761 gaccgaggtg gagaccttcg tcagccttgt gcgcaagaac cgggagccca ggttcctgga
     2821 ctacctctct gacctgtgtg tgtccaacca catcgccatc cccgtcaccc aagagctcat
     2881 ctgcaagtgt gtgctggacc ccaagaacag tgacattctc atccggaccg agcttcggcc
     2941 cgtgaaggag atggcccaat cccacgagta cctgagcatc gagtactcag aagaggaagt
     3001 gtggctcacg tggactgaca agaataacga gcatcatgag aagagtgtga ggcagctggc
     3061 ccaggaggcg cgggccggca acgcccacga cgagaatgtg ctcagctact acaggtacca
     3121 gctgaagctc tttgcccgca tgtgcttgga ccgccagtac ttggccatcg acgagatctc
     3181 ccagcagctg ggcgtggacc tgattttcct gtgcatggca gacgagatgc tgccctttga
     3241 cctgcgcgcc tccttctgcc acctgatgct gcacgtgcac gtggaccgtg acccccagga
     3301 gctggtcacg ccggtcaagt ttgcccgtct ctggactgag atccccacag ccatcaccat
     3361 caaggactat gattccaacc tcaacgcgtc ccgagatgac aagaagaaca agtttgccaa
     3421 caccatggag ttcgtggagg actacctcaa caatgtagtc agcgaggccg tgccctttgc
     3481 caacgaggag aagaacaagc tcacttttga ggtggtcagc ctggcgcaca atctcatcta
     3541 cttcggcttc tacagcttca gcgagctgct gcggctcact cgcacactgc tgggcatcat
     3601 cgactgtgtg caggggcccc cggccatgct gcaggcctat gaggaccccg gtggcaagaa
     3661 tgtgcggcgg tccatccagg gcgtggggca catgatgtcc accatggtgc tgagccgcaa
     3721 gcagtccgtc ttcagtgccc ccagcctgtc tgctggggcc agtgctgctg agccgctgga
     3781 cagaagcaag tttgaggaga atgaggacat tgtggtgatg gagaccaagc tgaagatcct
     3841 ggaaatcctt cagttcatcc tcaatgtccg cctggattac cgcatatcct acctgctgtc
     3901 tgtcttcaag aaggagtttg tggaggtgtt tcccatgcag gacagtgggg ctgatggcac
     3961 agcccctgcc ttcgactcta ccactgccaa catgaacctg gatcgcatcg gggagcaggc
     4021 ggaggccatg tttggagtgg ggaagacaag cagcatgctg gaggtggatg acgagggcgg
     4081 ccgcatgttc ctgcgcgtgc tcatccacct caccatgcac gactatgcgc cgctggtctc
     4141 gggtgccctg cagctgctct tcaagcactt cagccagcgc caggaggcca tgcacacctt
     4201 caagcaggtt cagctgctga tctcagcgca ggacgtggag aactacaagg tgatcaagtc
     4261 ggagctggac cggctgcgga ccatggtgga gaagtcagag ctgtgggtgg acaagaaggg
     4321 cagtggcaag ggtgaggagg tggaggcagg cgccgccaag gacaagaaag agcgtcccac
     4381 ggacgaggag ggctttctgc acccaccagg ggagaaaagc agtgagaact accagatcgt
     4441 caagggcatc ctggaaaggc tgaacaagat gtgcggggtt ggggagcaaa tgaggaagaa
     4501 gcagcaacgg ctgctgaaga acatggatgc ccacaaggtc atgctggacc tgctgcagat
     4561 cccctatgac aagggtgatg ccaagatgat ggagatcctg cgctacacgc accagttcct
     4621 gcagaagttc tgtgcaggga accccggcaa ccaggccctg ctgcacaaac acctgcacct
     4681 cttcctcacg ccagggctcc tggaggcaga gaccatgcag cacatcttcc tgaacaacta
     4741 tcagctctgc tccgagatca gcgagcctgt gttgcagcac ttcgtgcacc tgctggccac
     4801 gcacgggcgc catgtgcagt acctggactt cctgcacacc gtcattaagg ccgagggcaa
     4861 gtacgtcaag aagtgccagg acatgatcat gactgagctg accaatgcag gtgacgatgt
     4921 ggtcgtgttc tacaatgata aggcatcgct ggcccacctg ctggacatga tgaaggccgc
     4981 ccgcgacggc gtggaggacc acagccccct catgtaccac atttccctgg tggacctgct
     5041 ggccgcctgt gccgagggca aaaacgtcta cactgagatc aagtgcacct ccctgctgcc
     5101 gctggaggac gtggtgtctg tggtgacgca tgaggactgc atcactgagg tgaaaatggc
     5161 ctatgtgaac ttcgtgaacc actgctacgt ggacacggag gtggagatga aggagatcta
     5221 caccagcaac cacatctgga cgctctttga gaacttcacc ctggacatgg cccgggtctg
     5281 cagcaagcgt gagaagcgcg tggctgaccc caccttggag aagtacgtgc tgagcgttgt
     5341 gctggacacc atcaacgcct tcttcagctc cccattctct gagaacagca cttccctgca
     5401 gacacaccag acgattgtgg tgcagctgct gcagtctacc acacgcctcc tcgagtgtcc
     5461 gtggctacag cagcagcaca agggctccgt ggaggcctgc atccggaccc tcgccatggt
     5521 ggccaagggc cgggccatct tgctgcccat ggacctggat gcccacatca gctcgatgct
     5581 cagcagtgga gccagctgtg cagctgccgc ccagcggaac gcctccagct acaaggcaac
     5641 cacgcgggcc ttcccccgcg tcacccccac cgccaaccag tgggactaca agaacatcat
     5701 tgagaagctg caggacatca tcacagccct ggaggagcgg ctgaagcccc tggtacaggc
     5761 tgagctgtcc gtgctggtgg atgtcctgca ctggcctgag ctgctcttcc tggagggcag
     5821 tgaggcctac cagcgctgcg agagtggggg cttcctgtcc aagctgatcc agcacaccaa
     5881 ggacctcatg gagtcggagg agaagctgtg catcaaggtg ctgcggaccc tgcagcagat
     5941 gctgctcaag aagaccaagt acggggaccg gggcaaccag ctgcgcaaga tgctgctgca
     6001 aaactacctc cagaaccgga agtccacctc gcggggggac cttcccgacc ccataggcac
     6061 tggcctggac ccagactggt cggcaatcgc agccacccag tgccggctgg acaaggaggg
     6121 ggccaccaag ttggtatgcg acctcatcac cagcaccaag aacgagaaga tcttccagga
     6181 gagcatcggc ctggccatcc acctgctgga tggtggcaac acagagatcc agaaatcctt
     6241 ccacaacctg atgatgagtg acaagaagtc agagcgcttc ttcaaggtgc tgcacgaccg
     6301 catgaagcgg gcccagcagg agaccaagtc cacggtggca gtcaacatga atgacctggg
     6361 cagccagcca catgaggacc gcgagccagt cgaccccacc accaaaggcc gcgtggcctc
     6421 cttctcgata cctggctcct catcccgcta ctcgctgggc cccagcctgc gccgggggca
     6481 cgaggtgagc gaacgtgtgc agagcagtga gatgggcaca tccgtgctca tcatgcagcc
     6541 catcctgcgc tttctgcagc tgctgtgtga gaaccacaac cgggacctgc agaacttcct
     6601 gcgctgtcag aacaacaaaa ccaactacaa cttggtatgc gagacgctgc agttcctgga
     6661 catcatgtgc ggcagcacca cgggcggcct ggggctgctg gggctctaca tcaatgagga
     6721 caacgtgggc ctcgtcatcc agaccttgga gaccctcact gagtactgcc agggcccctg
     6781 ccatgagaac cagacttgca ttgtgactca cgagtccaat ggcatagaca tcatcaccgc
     6841 actgatcctc aatgacatca gccccctgtg caagtaccgc atggatctgg tgctgcagct
     6901 caaggacaat gcctccaagc tgctcctggc tctgatggag agccggcatg acagtgaaaa
     6961 tgctgagcga atcctcatca gcctgcggcc ccaggagctg gtggacgtca tcaagaaggc
     7021 ctacctgcag gaggaagagc gtgagaactc ggaggtgagc ccacgtgaag tgggccataa
     7081 catctatatc ctggcgctgc agctctccag gcacaataaa cagctgcagc acctgctgaa
     7141 gccggtgaag cgcattcaag aggaggaggc cgagggtatc tcttccatgc tcagcctcaa
     7201 caacaagcag ctgtcacaga tgctcaagtc ctcagcgcca gcacaggagg aggaggaaga
     7261 ccccctggcc tactatgaga accacacgtc ccagatcgag attgtgcggc aggaccgcag
     7321 catggagcag atcgtgttcc cagtgcccgg catctgccag ttcctgacgg aggaaaccaa
     7381 gcaccggctc ttcaccacta ctgagcagga cgagcagggc agcaaagtga gcgacttctt
     7441 cgaccagtcc tccttcctgc acaacgagat ggagtggcag cgcaagctcc gcagcatgcc
     7501 gctgatctac tggttctccc gccgcatgac cctgtggggc agcatctcct tcaacctggc
     7561 cgtgtttatc aacatcatca ttgccttctt ctacccttac atggagggcg cgtccacagg
     7621 cgtgctggac tcccctctca tctcattgct cttctggatc ctcatctgct tctccatcgc
     7681 ggccctgttc accaagcgct acagcatccg ccccctcatc gtggcgctca tcctgcgctc
     7741 catctactat ctgggcatcg ggcccacact caacatcctg ggtgccctca atctgaccaa
     7801 caagatcgtg tttgtggtga gcttcgtggg caaccgtggc accttcatcc ggggctataa
     7861 ggccatggtc atggacatgg aattcctcta ccacgtgggc tacatcctga ccagtgtcct
     7921 gggcctcttt gctcatgagc tgttctacag catcctgctc tttgacctca tctaccgcga
     7981 ggagacgctg ttcaacgtca tcaagagtgt gacccgcaat ggccgctcca tcctgctgac
     8041 agccctgctg gccctcatcc tggtctacct cttctccatc gtcggcttcc tcttcctcaa
     8101 ggatgacttc attctcgagg tcgaccggct gcccaacaac cactccacag ccagccccct
     8161 ggggatgcca catggagctg ctgcatttgt ggacacctgc agtggggaca agatggactg
     8221 tgtctcaggg ctctcggtgc ctgaggtcct ggaagaggac agggagctgg acagcacaga
     8281 gcgggcctgt gacactctgt tgatgtgcat cgtcactgtc atgaaccatg ggctacgcaa
     8341 cggtggtggc gtgggcgaca ttctccgcaa gccctccaaa gatgagtctc tcttcccagc
     8401 ccgagtggtc tatgacctcc tgttcttctt catcgtcatc atcattgtgc tgaacctcat
     8461 ctttggggta atcatcgaca ccttcgctga cctgcgtagt gagaagcaga agaaggagga
     8521 gattcttaag acgacatgct tcatctgtgg tctggagagg gacaagtttg ataacaagac
     8581 agtgtcattt gaggaacaca tcaagctgga gcacaacatg tggaactact tgtacttcat
     8641 tgtgctggtc cgcgtgaaga acaagaccga ctacacgggc cctgagagct acgtggccca
     8701 gatgatcaag aacaagaacc tggactggtt cccccggatg cgggccatgt cccttgtcag
     8761 caatgagggc gagggggagc agaatgagat tcggattctc caggacaagc tcaactccac
     8821 catgaagctg gtgtcccacc tcactgccca gctcaacgag ctcaaggagc agatgacgga
     8881 gcagcggaaa cgcaggcaac gcctaggctt tgtggatgtc cagaactgca ttagccgctg
     8941 aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc gtttaaaccc
     9001 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     9061 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     9121 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     9181 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     9241 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     9301 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     9361 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     9421 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     9481 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     9541 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     9601 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     9661 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     9721 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9781 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     9841 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     9901 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9961 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
    10021 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
    10081 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
    10141 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
    10201 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
    10261 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
    10321 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
    10381 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
    10441 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
    10501 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
    10561 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
    10621 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
    10681 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
    10741 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
    10801 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
    10861 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
    10921 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
    10981 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
    11041 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
    11101 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    11161 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    11221 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
    11281 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    11341 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    11401 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    11461 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    11521 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    11581 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    11641 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    11701 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    11761 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    11821 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    11881 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    11941 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    12001 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    12061 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    12121 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    12181 caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    12241 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    12301 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    12361 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    12421 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    12481 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    12541 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    12601 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    12661 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    12721 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    12781 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    12841 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    12901 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    12961 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    13021 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    13081 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    13141 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    13201 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    13261 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    13321 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    13381 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
    13441 ccctatggt
//
