LOCUS V025243 8060 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V025243 VERSION V025243 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 902..3295 /label="ITGB1(NM_002211)" /note="ITGB1(NM_002211)" /gene="ITGB1" CDS 3296..3547 /codon_start=1 /product="C-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /transl_table=1 /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK" /label="VC155" polyA_signal 3627..3851 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3897..4325 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4339..4668 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4735..5529 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5703..5824 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5873..5889) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5897..5913 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5921..5951) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5966..5987 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6275..6860) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7031..7891) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7892..7996) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 catgaattta caaccaattt tctggattgg actgatcagt tcagtttgct gtgtgtttgc 961 tcaaacagat gaaaatagat gtttaaaagc aaatgccaaa tcatgtggag aatgtataca 1021 agcagggcca aattgtgggt ggtgcacaaa ttcaacattt ttacaggaag gaatgcctac 1081 ttctgcacga tgtgatgatt tagaagcctt aaaaaagaag ggttgccctc cagatgacat 1141 agaaaatccc agaggctcca aagatataaa gaaaaataaa aatgtaacca accgtagcaa 1201 aggaacagca gagaagctca agccagagga tattactcag atccaaccac agcagttggt 1261 tttgcgatta agatcagggg agccacagac atttacatta aaattcaaga gagctgaaga 1321 ctatcccatt gacctctact accttatgga cctgtcttac tcaatgaaag acgatttgga 1381 gaatgtaaaa agtcttggaa cagatctgat gaatgaaatg aggaggatta cttcggactt 1441 cagaattgga tttggctcat ttgtggaaaa gactgtgatg ccttacatta gcacaacacc 1501 agctaagctc aggaaccctt gcacaagtga acagaactgc accagcccat ttagctacaa 1561 aaatgtgctc agtcttacta ataaaggaga agtatttaat gaacttgttg gaaaacagcg 1621 catatctgga aatttggatt ctccagaagg tggtttcgat gccatcatgc aagttgcagt 1681 ttgtggatca ctgattggct ggaggaatgt tacacggctg ctggtgtttt ccacagatgc 1741 cgggtttcac tttgctggag atgggaaact tggtggcatt gttttaccaa atgatggaca 1801 atgtcacctg gaaaataata tgtacacaat gagccattat tatgattatc cttctattgc 1861 tcaccttgtc cagaaactga gtgaaaataa tattcagaca atttttgcag ttactgaaga 1921 atttcagcct gtttacaagg agctgaaaaa cttgatccct aagtcagcag taggaacatt 1981 atctgcaaat tctagcaatg taattcagtt gatcattgat gcatacaatt ccctttcctc 2041 agaagtcatt ttggaaaacg gcaaattgtc agaaggcgta acaataagtt acaaatctta 2101 ctgcaagaac ggggtgaatg gaacagggga aaatggaaga aaatgttcca atatttccat 2161 tggagatgag gttcaatttg aaattagcat aacttcaaat aagtgtccaa aaaaggattc 2221 tgacagcttt aaaattaggc ctctgggctt tacggaggaa gtagaggtta ttcttcagta 2281 catctgtgaa tgtgaatgcc aaagcgaagg catccctgaa agtcccaagt gtcatgaagg 2341 aaatgggaca tttgagtgtg gcgcgtgcag gtgcaatgaa gggcgtgttg gtagacattg 2401 tgaatgcagc acagatgaag ttaacagtga agacatggat gcttactgca ggaaagaaaa 2461 cagttcagaa atctgcagta acaatggaga gtgcgtctgc ggacagtgtg tttgtaggaa 2521 gagggataat acaaatgaaa tttattctgg caaattctgc gagtgtgata atttcaactg 2581 tgatagatcc aatggcttaa tttgtggagg aaatggtgtt tgcaagtgtc gtgtgtgtga 2641 gtgcaacccc aactacactg gcagtgcatg tgactgttct ttggatacta gtacttgtga 2701 agccagcaac ggacagatct gcaatggccg gggcatctgc gagtgtggtg tctgtaagtg 2761 tacagatccg aagtttcaag ggcaaacgtg tgagatgtgt cagacctgcc ttggtgtctg 2821 tgctgagcat aaagaatgtg ttcagtgcag agccttcaat aaaggagaaa agaaagacac 2881 atgcacacag gaatgttcct attttaacat taccaaggta gaaagtcggg acaaattacc 2941 ccagccggtc caacctgatc ctgtgtccca ttgtaaggag aaggatgttg acgactgttg 3001 gttctatttt acgtattcag tgaatgggaa caacgaggtc atggttcatg ttgtggagaa 3061 tccagagtgt cccactggtc cagacatcat tccaattgta gctggtgtgg ttgctggaat 3121 tgttcttatt ggccttgcat tactgctgat atggaagctt ttaatgataa ttcatgacag 3181 aagggagttt gctaaatttg aaaaggagaa aatgaatgcc aaatgggaca cgggtgaaaa 3241 tcctatttat aagagtgccg taacaactgt ggtcaatccg aagtatgagg gaaaagacaa 3301 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt 3361 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 3421 cgacaaccac tacctgagct accagtccaa actgagcaaa gaccccaacg agaagcgcga 3481 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 3541 gtacaagtaa gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc 3601 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 3661 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 3721 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 3781 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 3841 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc 3901 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 3961 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 4021 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 4081 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 4141 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 4201 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 4261 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 4321 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 4381 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 4441 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 4501 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 4561 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta 4621 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga 4681 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt 4741 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat 4801 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag 4861 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac 4921 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac 4981 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc 5041 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg 5101 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag 5161 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat 5221 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag 5281 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc 5341 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg 5401 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg 5461 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag 5521 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat 5581 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc 5641 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc 5701 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 5761 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 5821 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 5881 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 5941 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 6001 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 6061 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 6121 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 6181 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 6241 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 6301 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 6361 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6421 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6481 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 6541 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 6601 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 6661 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 6721 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 6781 gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc 6841 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 6901 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 6961 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 7021 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 7081 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 7141 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 7201 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 7261 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 7321 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 7381 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 7441 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 7501 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 7561 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 7621 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 7681 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 7741 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 7801 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 7861 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 7921 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 7981 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag 8041 atctcccgat cccctatggt //