LOCUS       V039411                 8585 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039411
VERSION     V039411
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..4021
                     /label="IPO7(NM_006391)"
                     /note="IPO7(NM_006391)"
                     /gene="IPO7"
     CDS             4028..4117
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     polyA_signal    4152..4376
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4422..4850
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4864..5193
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5260..6054
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6228..6349
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6398..6414)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6422..6438
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6446..6476)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6491..6512
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6800..7385)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7556..8416)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8417..8521)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gaccccaaca ccattatcga ggccctgcgg ggcactatgg acccagccct
      961 gcgtgaggcc gcggagcgcc agctcaatga agcacacaag tctctgaatt ttgtctcaac
     1021 actgctccag attactatgt cggaacagct ggatttacct gtgagacagg caggtgttat
     1081 ctatctgaaa aatatgataa cacagtattg gcctgatcga gaaacagcac caggggatat
     1141 atccccttat actattccag aagaagatcg ccattgtatt cgagaaaata ttgtagaagc
     1201 cattatccat tctcctgagc tcatcagggt acagcttact acatgcattc atcacatcat
     1261 caaacatgat tatccaagcc gctggactgc cattgtggac aaaattggct tttatcttca
     1321 gtccgataac agtgcttgtt ggctaggaat tcttctttgc ctttatcagc ttgtgaaaaa
     1381 ttatgagtat aaaaaaccag aggagcggag tccattggta gcagcaatgc agcattttct
     1441 gccagttcta aaggatcgtt ttatccagct tctttctgac cagtctgatc agtctgtcct
     1501 catccagaaa cagatattca agatcttcta tgctcttgtt cagtatacac taccactgga
     1561 actgataaac caacagaacc tgacagaatg gatagaaatt ttaaagactg ttgtgaacag
     1621 ggatgtacct aatgaaacac ttcaagttga agaagatgat cgacctgagt taccatggtg
     1681 gaaatgcaag aagtgggcct tacatatttt agcaagactt tttgaaagat atggaagccc
     1741 tggcaatgtt tccaaggagt ataatgaatt tgctgaagta tttctgaagg catttgctgt
     1801 tggtgtccag caagttttat tgaaggtgtt atatcagtac aaggagaagc aatatatggc
     1861 tcctcgagtt ttacaacaga cattaaatta tattaatcaa ggagtttctc atgctctcac
     1921 ctggaagaat ctgaagcccc atatacaagg cattatccaa gatgttattt ttccattgat
     1981 gtgctataca gatgctgatg aggaactttg gcaagaagac ccttacgaat atatacgcat
     2041 gaagtttgat gtgtttgaag atttcatttc tcctaccact gctgcccaga cacttttgtt
     2101 tacagcctgt agtaagagga aagaggtact gcaaaagact atgggatttt gttaccagat
     2161 tcttacagaa ccaaatgctg accctcgaaa aaaagatgga gccctgcata tgattggctc
     2221 tttagctgaa atacttctga agaaaaagat ctataaagat cagatggaat acatgttgca
     2281 gaatcatgta ttccctctct tcagcagtga actaggctac atgagagcaa gggcttgctg
     2341 ggtacttcac tatttttgtg aagtgaagtt caaaagtgat cagaaccttc aaacagcctt
     2401 agagctaaca agaagatgtc tgattgatga tagagaaatg cctgtgaaag tggaagctgc
     2461 cattgccctt caagtattga tcagcaatca agaaaaagct aaagaatata tcacaccatt
     2521 catcagacct gtaatgcagg ctcttcttca cattataaga gaaacagaaa atgatgacct
     2581 taccaatgta attcagaaaa tgatctgtga atatagtgaa gaagttactc ctattgcagt
     2641 agaaatgaca caacatttgg caatgacatt taaccaagta atccagacgg ggccagatga
     2701 agaaggtagt gatgacaaag cagttactgc tatgggaatt ctgaatacaa ttgatacact
     2761 tcttagtgta gttgaagatc ataaagagat aacccaacag cttgagggaa tctgcttaca
     2821 ggtcattggt actgttttac aacagcatgt cttagaattc tatgaggaga tcttctcttt
     2881 agcgcacagt ttgacatgtc aacaagtgtc tccacagatg tggcagctac taccccttgt
     2941 atttgaagtc tttcagcaag atggctttga ttactttaca gatatgatgc ccctccttca
     3001 taattatgta acagttgata cagacacact tctgtctgac accaagtatc ttgaaatgat
     3061 atacagtatg tgcaaaaagg ttcttacagg agttgcagga gaagatgcag agtgtcatgc
     3121 agcaaaattg ttagaggtca tcattctgca gtgcaaaggg cgtggcattg accagtgcat
     3181 tcccttattc gtggaagcag ccttagaaag actgacaaga gaggttaaga caagtgaact
     3241 tcgaactatg tgtctgcaag ttgcaattgc agctttgtat tataatccac acctactact
     3301 caatacctta gaaaatcttc gcttccctaa taatgttgaa ccagttacaa atcattttat
     3361 tacacagtgg cttaatgatg ttgactgttt cttggggctt catgacagaa agatgtgtgt
     3421 tctcggactc tgtgctctta ttgatatgga acagataccc caagttttaa atcaggtttc
     3481 tggacagatt ttgccggctt ttatcctttt atttaacgga ttgaaaagag catatgcctg
     3541 ccatgcagaa catgagaatg acagtgatga tgatgatgaa gctgaagatg atgatgaaac
     3601 cgaggaactg gggagtgatg aagatgatat tgatgaagat gggcaagaat atttggagat
     3661 tctggctaag caggctggtg aagatggaga tgatgaagat tgggaagaag atgatgctga
     3721 agagactgct ctggaaggct attccacaat cattgatgat gaagataacc ctgttgatga
     3781 gtatcagata tttaaagcta tctttcaaac tattcaaaat cgtaatcctg tgtggtatca
     3841 ggcactgact cacggtctta atgaagaaca aagaaaacag ttacaggaca tagcaactct
     3901 ggctgatcaa agaagagcag cccatgaatc caaaatgatt gagaagcatg gaggatacaa
     3961 attcagtgct ccagttgtgc caagttcttt caattttgga ggcccagcac cagggatgaa
     4021 tctcgaggaa caaaaactaa tatcagagga agatttggaa cagaaactaa tcagtgaaga
     4081 agatttagag caaaagttaa tttccgagga ggacttatga gggcccgttt aaacccgctg
     4141 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     4201 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4261 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     4321 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     4381 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     4441 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4501 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4561 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4621 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4681 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4741 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4801 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     4861 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4921 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4981 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     5041 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     5101 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     5161 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     5221 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     5281 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     5341 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     5401 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     5461 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     5521 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     5581 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     5641 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     5701 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     5761 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     5821 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     5881 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     5941 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     6001 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     6061 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     6121 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     6181 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     6241 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6301 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     6361 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6421 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6481 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6541 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6601 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6661 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6721 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6781 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6841 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6901 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6961 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7021 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7081 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7141 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7201 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7261 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7321 ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7381 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7441 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7501 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7561 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7621 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7681 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7741 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7801 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7861 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7921 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7981 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8041 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8101 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8161 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8221 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8281 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8341 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8401 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8461 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8521 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     8581 atggt
//
