LOCUS V031408 6428 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031408 VERSION V031408 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..1915 /label="IGLON5(NM_001101372)" /note="IGLON5(NM_001101372)" /gene="IGLON5" polyA_signal 1995..2219 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2265..2693 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2707..3036 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3103..3897 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4071..4192 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4241..4257) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4265..4281 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4289..4319) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4334..4355 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4643..5228) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5399..6259) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6260..6364) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg cccccccctg cgcccggggc ccggctccgg cttctcgccg ccgccgccct 961 ggccggcttg gccgtcatca gccgagggct gctctcccag agcctggagt tcaactctcc 1021 tgccgacaac tacacagtgt gtgaaggtga caacgccacc ctcagctgct tcatcgacga 1081 gcacgtgacc cgcgtggcct ggctgaaccg ctccaacatc ctgtatgccg gcaatgaccg 1141 ctggaccagc gacccgcggg tgcggctgct catcaacacc cccgaggagt tctccatcct 1201 catcaccgag gtggggctcg gcgacgaggg cctctacacc tgctccttcc agacccgcca 1261 ccagccgtac accactcagg tctacctcat tgtccacgtc cctgcccgca ttgtgaacat 1321 ctcgtcgcct gtgacggtga atgagggggg caatgtgaac ctgctttgcc tggccgtggg 1381 gcggccagag cccacggtca cctggagaca gctccgagac ggcttcacct cggagggaga 1441 gatcctggag atctctgaca tccagcgggg ccaggccggg gagtatgagt gcgtgactca 1501 caacggggtt aactcggcgc ccgacagccg ccgcgtgctg gtcacagtca actatcctcc 1561 gaccatcacg gacgtgacca gcgcccgcac cgcgctgggc cgggccgccc tcctgcgctg 1621 cgaagccatg gcggttcccc ccgcggattt ccagtggtac aaggatgaca gactgctgag 1681 cagcggcacg gccgaaggcc tgaaggtgca gacggagcgc acccgctcga tgcttctctt 1741 tgccaacgtg agcgcccggc attacggcaa ctatacgtgt cgcgccgcca accgactggg 1801 agcgtccagc gcctccatgc ggctcctgcg cccaggatcc ctggagaact cagccccgag 1861 gcccccaggg ctcctggccc tcctctccgc cctgggctgg ctgtggtgga gaatgtagga 1921 attctgcaga tatccagcac agtggcggcc gctcgagtct agagggcccg tttaaacccg 1981 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt 2041 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 2101 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 2161 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc 2221 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg 2281 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 2341 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 2401 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 2461 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 2521 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 2581 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 2641 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg 2701 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg 2761 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 2821 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 2881 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 2941 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag 3001 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc 3061 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga 3121 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa 3181 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt 3241 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg 3301 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa 3361 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac 3421 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt 3481 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact 3541 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg 3601 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg 3661 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc 3721 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt 3781 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc 3841 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg 3901 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 3961 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 4021 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 4081 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 4141 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 4201 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 4261 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 4321 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 4381 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 4441 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 4501 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 4561 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 4621 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 4681 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 4741 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 4801 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 4861 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 4921 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 4981 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 5041 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 5101 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 5161 aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 5221 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 5281 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 5341 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 5401 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 5461 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 5521 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 5581 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 5641 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 5701 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 5761 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 5821 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 5881 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 5941 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 6001 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 6061 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 6121 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 6181 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 6241 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 6301 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 6361 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc 6421 cctatggt //