LOCUS       V026662                 7429 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026662
VERSION     V026662
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..2868
                     /label="HSPA8(NM_006597)"
                     /note="HSPA8(NM_006597)"
                     /gene="HSPA8"
     CDS             2875..2901
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2905..2931
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2935..2961
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    2996..3220
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3266..3694
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3708..4037
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4104..4898
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5072..5193
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5242..5258)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5266..5282
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5290..5320)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5335..5356
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5644..6229)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6400..7260)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7261..7365)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atgtccaagg gacctgcagt tggtattgat
      961 cttggcacca cctactcttg tgtgggtgtt ttccagcacg gaaaagtcga gataattgcc
     1021 aatgatcagg gaaaccgaac cactccaagc tatgtcgcct ttacggacac tgaacggttg
     1081 atcggtgatg ccgcaaagaa tcaagttgca atgaacccca ccaacacagt ttttgatgcc
     1141 aaacgtctga ttggacgcag atttgatgat gctgttgtcc agtctgatat gaaacattgg
     1201 ccctttatgg tggtgaatga tgctggcagg cccaaggtcc aagtagaata caagggagag
     1261 accaaaagct tctatccaga ggaggtgtct tctatggttc tgacaaagat gaaggaaatt
     1321 gcagaagcct accttgggaa gactgttacc aatgctgtgg tcacagtgcc agcttacttt
     1381 aatgactctc agcgtcaggc taccaaagat gctggaacta ttgctggtct caatgtactt
     1441 agaattatta atgagccaac tgctgctgct attgcttacg gcttagacaa aaaggttgga
     1501 gcagaaagaa acgtgctcat ctttgacctg ggaggtggca cttttgatgt gtcaatcctc
     1561 actattgagg atggaatctt tgaggtcaag tctacagctg gagacaccca cttgggtgga
     1621 gaagattttg acaaccgaat ggtcaaccat tttattgctg agtttaagcg caagcataag
     1681 aaggacatca gtgagaacaa gagagctgta agacgcctcc gtactgcttg tgaacgtgct
     1741 aagcgtaccc tctcttccag cacccaggcc agtattgaga tcgattctct ctatgaagga
     1801 atcgacttct atacctccat tacccgtgcc cgatttgaag aactgaatgc tgacctgttc
     1861 cgtggcaccc tggacccagt agagaaagcc cttcgagatg ccaaactaga caagtcacag
     1921 attcatgata ttgtcctggt tggtggttct actcgtatcc ccaagattca gaagcttctc
     1981 caagacttct tcaatggaaa agaactgaat aagagcatca accctgatga agctgttgct
     2041 tatggtgcag ctgtccaggc agccatcttg tctggagaca agtctgagaa tgttcaagat
     2101 ttgctgctct tggatgtcac tcctctttcc cttggtattg aaactgctgg tggagtcatg
     2161 actgtcctca tcaagcgtaa taccaccatt cctaccaagc agacacagac cttcactacc
     2221 tattctgaca accagcctgg tgtgcttatt caggtttatg aaggcgagcg tgccatgaca
     2281 aaggataaca acctgcttgg caagtttgaa ctcacaggca tacctcctgc accccgaggt
     2341 gttcctcaga ttgaagtcac ttttgacatt gatgccaatg gtatactcaa tgtctctgct
     2401 gtggacaaga gtacgggaaa agagaacaag attactatca ctaatgacaa gggccgtttg
     2461 agcaaggaag acattgaacg tatggtccag gaagctgaga agtacaaagc tgaagatgag
     2521 aagcagaggg acaaggtgtc atccaagaat tcacttgagt cctatgcctt caacatgaaa
     2581 gcaactgttg aagatgagaa acttcaaggc aagattaacg atgaggacaa acagaagatt
     2641 ctggacaagt gtaatgaaat tatcaactgg cttgataaga atcagactgc tgagaaggaa
     2701 gaatttgaac atcaacagaa agagctggag aaagtttgca accccatcat caccaagctg
     2761 taccagagtg caggaggcat gccaggagga atgcctgggg gatttcctgg tggtggagct
     2821 cctccctctg gtggtgcttc ctcagggccc accattgaag aggttgatct cgagtaccca
     2881 tacgacgtac ctgattatgc aacttatcct tatgatgtac ctgattatgc tagctatcct
     2941 tatgatgtcc cagactacgc atgagggccc gtttaaaccc gctgatcagc ctcgactgtg
     3001 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     3061 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     3121 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     3181 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     3241 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3301 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3361 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3421 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3481 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3541 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     3601 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     3661 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     3721 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3781 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3841 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     3901 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     3961 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4021 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
     4081 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     4141 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     4201 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     4261 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     4321 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     4381 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     4441 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     4501 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     4561 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     4621 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     4681 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     4741 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     4801 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     4861 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     4921 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     4981 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     5041 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     5101 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5161 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     5221 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5281 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     5341 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     5401 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     5461 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     5521 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5581 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5641 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5701 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5761 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5821 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5881 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5941 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6001 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6061 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     6121 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6181 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6241 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6301 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6361 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     6421 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6481 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6541 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6601 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6661 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6721 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     6781 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6841 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6901 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6961 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7021 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7081 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7141 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7201 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7261 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7321 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7381 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggt
//
