LOCUS       V025595                 7999 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025595
VERSION     V025595
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..3492
                     /label="HSP90AA1(NM_001017963)"
                     /note="HSP90AA1(NM_001017963)"
                     /gene="HSP90AA1"
     CDS             3502..3519
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     polyA_signal    3566..3790
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3836..4264
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4278..4607
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4674..5468
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5642..5763
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5812..5828)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5836..5852
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5860..5890)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5905..5926
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6214..6799)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6970..7830)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7831..7935)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atgcccccgt gttcgggcgg ggacggctcc
      961 acccctcctg ggccctccct tcgggacagg gactgtcccg cccagagtgc tgaatacccg
     1021 cgcgaccgtc tggatccccg cccaggaagc ccctctgaag cctcctcgcc gccgtttctg
     1081 agaagcaggg cacctgttaa ctggtaccaa gaaaaggccc aagtgtttct ctggcatctg
     1141 atggtgtctg gatccaccac tctactctgt ctctggaaac agcccttcca cgtctctgca
     1201 ttccctgtca ccgcgtcact ggccttcaga cagagccaag gtgcagggca acacctctac
     1261 aaggatctgc agccatttat attgcttagg ctactgatgc ctgaggaaac ccagacccaa
     1321 gaccaaccga tggaggagga ggaggttgag acgttcgcct ttcaggcaga aattgcccag
     1381 ttgatgtcat tgatcatcaa tactttctac tcgaacaaag agatctttct gagagagctc
     1441 atttcaaatt catcagatgc attggacaaa atccggtatg aaagcttgac agatcccagt
     1501 aaattagact ctgggaaaga gctgcatatt aaccttatac cgaacaaaca agatcgaact
     1561 ctcactattg tggatactgg aattggaatg accaaggctg acttgatcaa taaccttggt
     1621 actatcgcca agtctgggac caaagcgttc atggaagctt tgcaggctgg tgcagatatc
     1681 tctatgattg gccagttcgg tgttggtttt tattctgctt atttggttgc tgagaaagta
     1741 actgtgatca ccaaacataa cgatgatgag cagtacgctt gggagtcctc agcaggggga
     1801 tcattcacag tgaggacaga cacaggtgaa cctatgggtc gtggaacaaa agttatccta
     1861 cacctgaaag aagaccaaac tgagtacttg gaggaacgaa gaataaagga gattgtgaag
     1921 aaacattctc agtttattgg atatcccatt actctttttg tggagaagga acgtgataaa
     1981 gaagtaagcg atgatgaggc tgaagaaaag gaagacaaag aagaagaaaa agaaaaagaa
     2041 gagaaagagt cggaagacaa acctgaaatt gaagatgttg gttctgatga ggaagaagaa
     2101 aagaaggatg gtgacaagaa gaagaagaag aagattaagg aaaagtacat cgatcaagaa
     2161 gagctcaaca aaacaaagcc catctggacc agaaatcccg acgatattac taatgaggag
     2221 tacggagaat tctataagag cttgaccaat gactgggaag atcacttggc agtgaagcat
     2281 ttttcagttg aaggacagtt ggaattcaga gcccttctat ttgtcccacg acgtgctcct
     2341 tttgatctgt ttgaaaacag aaagaaaaag aacaacatca aattgtatgt acgcagagtt
     2401 ttcatcatgg ataactgtga ggagctaatc cctgaatatc tgaacttcat tagaggggtg
     2461 gtagactcgg aggatctccc tctaaacata tcccgtgaga tgttgcaaca aagcaaaatt
     2521 ttgaaagtta tcaggaagaa tttggtcaaa aaatgcttag aactctttac tgaactggcg
     2581 gaagataaag agaactacaa gaaattctat gagcagttct ctaaaaacat aaagcttgga
     2641 atacacgaag actctcaaaa tcggaagaag ctttcagagc tgttaaggta ctacacatct
     2701 gcctctggtg atgagatggt ttctctcaag gactactgca ccagaatgaa ggagaaccag
     2761 aaacatatct attatatcac aggtgagacc aaggaccagg tagctaactc agcctttgtg
     2821 gaacgtcttc ggaaacatgg cttagaagtg atctatatga ttgagcccat tgatgagtac
     2881 tgtgtccaac agctgaagga atttgagggg aagactttag tgtcagtcac caaagaaggc
     2941 ctggaacttc cagaggatga agaagagaaa aagaagcagg aagagaaaaa aacaaagttt
     3001 gagaacctct gcaaaatcat gaaagacata ttggagaaaa aagttgaaaa ggtggttgtg
     3061 tcaaaccgat tggtgacatc tccatgctgt attgtcacaa gcacatatgg ctggacagca
     3121 aacatggaga gaatcatgaa agctcaagcc ctaagagaca actcaacaat gggttacatg
     3181 gcagcaaaga aacacctgga gataaaccct gaccattcca ttattgagac cttaaggcaa
     3241 aaggcagagg ctgataagaa cgacaagtct gtgaaggatc tggtcatctt gctttatgaa
     3301 actgcgctcc tgtcttctgg cttcagtctg gaagatcccc agacacatgc taacaggatc
     3361 tacaggatga tcaaacttgg tctgggtatt gatgaagatg accctactgc tgatgatacc
     3421 agtgctgctg taactgaaga aatgccaccc cttgaaggag atgacgacac atcacgcatg
     3481 gaagaagtag acgcggccgc acatcaccat catcaccatt gactcgagtc tagagggccc
     3541 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     3601 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     3661 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     3721 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     3781 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     3841 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3901 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3961 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4021 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4081 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4141 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4201 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4261 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     4321 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     4381 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     4441 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4501 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     4561 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     4621 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     4681 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     4741 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     4801 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     4861 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     4921 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     4981 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     5041 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     5101 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     5161 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     5221 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     5281 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     5341 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     5401 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     5461 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     5521 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     5581 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     5641 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5701 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5761 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     5821 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     5881 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     5941 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6001 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6061 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6121 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6181 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6241 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6301 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6361 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6421 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6481 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6541 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6601 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6661 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6721 atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc agattacgcg
     6781 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6841 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6901 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6961 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7021 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7081 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7141 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7201 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7261 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7321 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7381 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7441 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7501 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7561 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7621 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7681 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7741 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7801 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7861 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7921 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     7981 tctcccgatc ccctatggt
//
