LOCUS       V020292                 8485 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020292
VERSION     V020292
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             932..3964
                     /label="HDAC9(NM_058176)"
                     /note="HDAC9(NM_058176)"
                     /gene="HDAC9"
     CDS             3986..4051
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    4080..4304
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4350..4778
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4792..5121
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5188..5982
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6158..6279
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6328..6344)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6352..6368
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6376..6406)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6421..6442
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6730..7318)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7489..8349)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8350..8454)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccgccac catgcacagt atgatcagct cagtggatgt
      961 gaagtcagaa gttcctgtgg gcctggagcc catctcacct ttagacctaa ggacagacct
     1021 caggatgatg atgcccgtgg tggaccctgt tgtccgtgag aagcaattgc agcaggaatt
     1081 acttcttatc cagcagcagc aacaaatcca gaagcagctt ctgatagcag agtttcagaa
     1141 acagcatgag aacttgacac ggcagcacca ggctcagctt caggagcata tcaaggaact
     1201 tctagccata aaacagcaac aagaactcct agaaaaggag cagaaactgg agcagcagag
     1261 gcaagaacag gaagtagaga ggcatcgcag agaacagcag cttcctcctc tcagaggcaa
     1321 agatagagga cgagaaaggg cagtggcaag tacagaagta aagcagaagc ttcaagagtt
     1381 cctactgagt aaatcagcaa cgaaagacac tccaactaat ggaaaaaatc attccgtgag
     1441 ccgccatccc aagctctggt acacggctgc ccaccacaca tcattggatc aaagctctcc
     1501 accccttagt ggaacatctc catcctacaa gtacacatta ccaggagcac aagatgcaaa
     1561 ggatgatttc ccccttcgaa aaactgcctc tgagcccaac ttgaaggtgc ggtccaggtt
     1621 aaaacagaaa gtggcagaga ggagaagcag ccccttactc aggcggaagg atggaaatgt
     1681 tgtcacttca ttcaagaagc gaatgtttga ggtgacagaa tcctcagtca gtagcagttc
     1741 tccaggctct ggtcccagtt caccaaacaa tgggccaact ggaagtgtta ctgaaaatga
     1801 gacttcggtt ttgcccccta cccctcatgc cgagcaaatg gtttcacagc aacgcattct
     1861 aattcatgaa gattccatga acctgctaag tctttatacc tctccttctt tgcccaacat
     1921 taccttgggg cttcccgcag tgccatccca gctcaatgct tcgaattcac tcaaagaaaa
     1981 gcagaagtgt gagacgcaga cgcttaggca aggtgttcct ctgcctgggc agtatggagg
     2041 cagcatcccg gcatcttcca gccaccctca tgttacttta gagggaaagc cacccaacag
     2101 cagccaccag gctctcctgc agcatttatt attgaaagaa caaatgcgac agcaaaagct
     2161 tcttgtagct ggtggagttc ccttacatcc tcagtctccc ttggcaacaa aagagagaat
     2221 ttcacctggc attagaggta cccacaaatt gccccgtcac agacccctga accgaaccca
     2281 gtctgcacct ttgcctcaga gcacgttggc tcagctggtc attcaacagc aacaccagca
     2341 attcttggag aagcagaagc aataccagca gcagatccac atgaacaaac tgctttcgaa
     2401 atctattgaa caactgaagc aaccaggcag tcaccttgag gaagcagagg aagagcttca
     2461 gggggaccag gcgatgcagg aagacagagc gccctctagt ggcaacagca ctaggagcga
     2521 cagcagtgct tgtgtggatg acacactggg acaagttggg gctgtgaagg tcaaggagga
     2581 accagtggac agtgatgaag atgctcagat ccaggaaatg gaatctgggg agcaggctgc
     2641 ttttatgcaa cagcctttcc tggaacccac gcacacacgt gcgctctctg tgcgccaagc
     2701 tccgctggct gcggttggca tggatggatt agagaaacac cgtctcgtct ccaggactca
     2761 ctcttcccct gctgcctctg ttttacctca cccagcaatg gaccgccccc tccagcctgg
     2821 ctctgcaact ggaattgcct atgacccctt gatgctgaaa caccagtgcg tttgtggcaa
     2881 ttccaccacc caccctgagc atgctggacg aatacagagt atctggtcac gactgcaaga
     2941 aactgggctg ctaaataaat gtgagcgaat tcaaggtcga aaagccagcc tggaggaaat
     3001 acagcttgtt cattctgaac atcactcact gttgtatggc accaaccccc tggacggaca
     3061 gaagctggac cccaggatac tcctaggtga tgactctcaa aagttttttt cctcattacc
     3121 ttgtggtgga cttggggtgg acagtgacac catttggaat gagctacact cgtccggtgc
     3181 tgcacgcatg gctgttggct gtgtcatcga gctggcttcc aaagtggcct caggagagct
     3241 gaagaatggg tttgctgttg tgaggccccc tggccatcac gctgaagaat ccacagccat
     3301 ggggttctgc ttttttaatt cagttgcaat taccgccaaa tacttgagag accaactaaa
     3361 tataagcaag atattgattg tagatctgga tgttcaccat ggaaacggta cccagcaggc
     3421 cttttatgct gaccccagca tcctgtacat ttcactccat cgctatgatg aagggaactt
     3481 tttccctggc agtggagccc caaatgaggt tggaacaggc cttggagaag ggtacaatat
     3541 aaatattgcc tggacaggtg gccttgatcc tcccatggga gatgttgagt accttgaagc
     3601 attcaggacc atcgtgaagc ctgtggccaa agagtttgat ccagacatgg tcttagtatc
     3661 tgctggattt gatgcattgg aaggccacac ccctcctcta ggagggtaca aagtgacggc
     3721 aaaatgtttt ggtcatttga cgaagcaatt gatgacattg gctgatggac gtgtggtgtt
     3781 ggctctagaa ggaggacatg atctcacagc catctgtgat gcatcagaag cctgtgtaaa
     3841 tgcccttcta ggaaatgagc tggagccact tgcagaagat attctccacc aaagcccgaa
     3901 tatgaatgct gttatttctt tacagaagat cattgaaatt caaagtatgt ctttaaagtt
     3961 ctctctcgag tctagagggc ccttcgacta caaagaccat gacggtgatt ataaagatca
     4021 tgacatcgac tacaaggatg acgatgacaa gtgagtttaa acccgctgat cagcctcgac
     4081 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     4141 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     4201 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     4261 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     4321 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     4381 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4441 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4501 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4561 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4621 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4681 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4741 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     4801 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4861 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4921 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     4981 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5041 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     5101 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     5161 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     5221 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     5281 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     5341 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     5401 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     5461 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     5521 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     5581 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     5641 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     5701 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     5761 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     5821 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     5881 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     5941 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     6001 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     6061 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     6121 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     6181 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6241 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     6301 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6361 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6421 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6481 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6541 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6601 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6661 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6721 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6781 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6841 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6901 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6961 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7021 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7081 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7141 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7201 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7261 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7321 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7381 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7441 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7501 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7561 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7621 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7681 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7741 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7801 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7861 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7921 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7981 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8041 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8101 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8161 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8221 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8281 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8341 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8401 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8461 tccccgaaaa gtgccacctg acgtc
//
