LOCUS V031365 6914 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031365 VERSION V031365 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..2404 /label="HDAC2(NM_001527)" /note="HDAC2(NM_001527)" /gene="HDAC2" CDS 2411..2476 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 2514..2738 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2784..3212 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3226..3555 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3622..4416 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4590..4711 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4760..4776) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4784..4800 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4808..4838) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4853..4874 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5162..5747) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5918..6778) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6779..6883) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggcgtaca gtcaaggagg 961 cggcaaaaaa aaagtctgct actactacga cggtgatatt ggaaattatt attatggaca 1021 gggtcatccc atgaagcctc atagaatccg catgacccat aacttgctgt taaattatgg 1081 cttatacaga aaaatggaaa tatataggcc ccataaagcc actgccgaag aaatgacaaa 1141 atatcacagt gatgagtata tcaaatttct acggtcaata agaccagata acatgtctga 1201 gtatagtaag cagatgcaga gatttaatgt tggagaagat tgtccagtgt ttgatggact 1261 ctttgagttt tgtcagctct caactggcgg ttcagttgct ggagctgtga agttaaaccg 1321 acaacagact gatatggctg ttaattgggc tggaggatta catcatgcta agaaatcaga 1381 agcatcagga ttctgttacg ttaatgatat tgtgcttgcc atccttgaat tactaaagta 1441 tcatcagaga gtcttatata ttgatataga tattcatcat ggtgatggtg ttgaagaagc 1501 tttttataca acagatcgtg taatgacggt atcattccat aaatatgggg aatactttcc 1561 tggcacagga gacttgaggg atattggtgc tggaaaaggc aaatactatg ctgtcaattt 1621 tccaatgaga gatggtatag atgatgagtc atatgggcag atatttaagc ctattatctc 1681 aaaggtgatg gagatgtatc aacctagtgc tgtggtatta cagtgtggtg cagactcatt 1741 atctggtgat agactgggtt gtttcaatct aacagtcaaa ggtcatgcta aatgtgtaga 1801 agttgtaaaa acttttaact taccattact gatgcttgga ggaggtggct acacaatccg 1861 taatgttgct cgatgttgga catatgagac tgcagttgcc cttgattgtg agattcccaa 1921 tgagttgcca tataatgatt actttgagta ttttggacca gacttcaaac tgcatattag 1981 tccttcaaac atgacaaacc agaacactcc agaatatatg gaaaagataa aacagcgttt 2041 gtttgaaaat ttgcgcatgt tacctcatgc acctggtgtc cagatgcaag ctattccaga 2101 agatgctgtt catgaagaca gtggagatga agatggagaa gatccagaca agagaatttc 2161 tattcgagca tcagacaagc ggatagcttg tgatgaagaa ttctcagatt ctgaggatga 2221 aggagaagga ggtcgaagaa atgtggctga tcataagaaa ggagcaaaga aagctagaat 2281 tgaagaagat aagaaagaaa cagaggacaa aaaaacagac gttaaggaag aagataaatc 2341 caaggacaac agtggtgaaa aaacagatac caaaggaacc aaatcagaac agctcagcaa 2401 ccccctcgag gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa 2461 ggatgacgat gacaagtagt gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc 2521 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 2581 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 2641 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 2701 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag 2761 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 2821 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 2881 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 2941 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 3001 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 3061 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 3121 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 3181 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg 3241 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 3301 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 3361 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 3421 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 3481 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 3541 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag 3601 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 3661 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 3721 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 3781 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg 3841 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 3901 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 3961 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 4021 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 4081 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 4141 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 4201 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 4261 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 4321 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 4381 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga 4441 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg 4501 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg 4561 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca 4621 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 4681 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct 4741 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 4801 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 4861 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 4921 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 4981 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 5041 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 5101 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 5161 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 5221 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 5281 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 5341 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 5401 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 5461 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 5521 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 5581 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 5641 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt 5701 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 5761 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 5821 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 5881 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 5941 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 6001 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 6061 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 6121 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 6181 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 6241 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 6301 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 6361 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 6421 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 6481 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 6541 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 6601 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 6661 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 6721 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 6781 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 6841 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 6901 tgccacctga cgtc //