LOCUS V046868 6484 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V046868 VERSION V046868 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 931..1923 /label="Gpr18(NM_182806)" /note="Gpr18(NM_182806)" /gene="Gpr18" CDS 1930..1956 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1960..1986 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1990..2016 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" polyA_signal 2051..2275 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2321..2749 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2763..3092 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3159..3953 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4127..4248 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4297..4313) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4321..4337 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4345..4375) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4390..4411 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4699..5284) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5455..6315) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6316..6420) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cactagtcca gtgtggtgga attcgccacc atggccaccc tgagcaatca caaccagctt 961 gatctttcta atggctcaca cccagaggaa tacaaaatcg cagccctagt cttctacagc 1021 tgcatcttcc tgattgggct gtttgttaat gtcactgcgt tgtgggtttt cagctgtacg 1081 accaagaaaa gaaccacagt gaccatctac atgatgaacg ttgcactact ggacctcgta 1141 tttatactca gtctgccctt tcggatgttt tactatgcaa aaggcgagtg gccatttgga 1201 gagtacttct gccacattct tggggccctg gtggtgtttt acccaagcct cgctctgtgg 1261 cttcttgctt tcattagtgc tgacagatac atggccatcg tacagccaaa atatgccaag 1321 gagctgaaga acaccggcaa ggccgtgctt gcgtgtgggg gggtctgggt aatgaccctg 1381 accaccactg tccccctgct actgctctac gaagacccag acaaggcctc ctccccagcc 1441 acctgcctga agatctccga catcacccac ttaaaagctg tcaacgtgct caacttcacg 1501 cgactcatat ttttcttcct gatccctttg ttcatcatga tcgggtgcta cgtggtcatc 1561 attcacagtc tcctccgagg gcagacgtct aagctgaagc ccaaggtcaa ggagaagtcc 1621 atacggatca tcatgaccct cctgctgcag gtgctcgtct gcttcgtgcc cttccacatc 1681 tgctttgccg tcctgatgct acaaggacag gagaacagct atagcccctg gggagccttc 1741 accaccttcc tcatgaacct cagcacctgt ctcgatgtag tcctctacta catcgtttcc 1801 aaacagttcc aggctcgagt catcagcgtc atgctgtacc gcaattacct tcgcagtgtt 1861 cgcagaaaaa gtgtccgatc gggcagttta cggtcactta gcaacatgaa cagtgagatg 1921 cttctcgagt acccatacga cgtacctgat tatgcaactt atccttatga tgtacctgat 1981 tatgctagct atccttatga tgtcccagac tacgcatgag ggcccgttta aacccgctga 2041 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct 2101 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca 2161 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag 2221 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct 2281 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca 2341 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 2401 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 2461 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 2521 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 2581 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 2641 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 2701 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga 2761 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 2821 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 2881 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 2941 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 3001 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 3061 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 3121 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 3181 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 3241 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 3301 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 3361 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 3421 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 3481 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 3541 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 3601 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 3661 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 3721 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 3781 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 3841 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 3901 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 3961 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 4021 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 4081 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 4141 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 4201 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 4261 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 4321 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 4381 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 4441 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 4501 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 4561 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 4621 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 4681 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 4741 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 4801 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 4861 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 4921 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 4981 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 5041 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 5101 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 5161 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 5221 caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 5281 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 5341 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 5401 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 5461 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 5521 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 5581 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 5641 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 5701 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 5761 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 5821 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 5881 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 5941 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 6001 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 6061 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 6121 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 6181 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 6241 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 6301 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 6361 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 6421 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta 6481 tggt //