LOCUS       V037403                 9194 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037403
VERSION     V037403
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             884..1591
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1628..4714
                     /label="GOLGA2(NM_001366244)"
                     /note="GOLGA2(NM_001366244)"
                     /gene="GOLGA2"
     polyA_signal    4761..4985
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      5031..5459
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5473..5802
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5869..6663
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6837..6958
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7007..7023)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7031..7047
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7055..7085)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7100..7121
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7409..7994)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8165..9025)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9026..9130)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgct accggtcgcc accatggtga gcaagggcga
      901 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      961 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     1021 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     1081 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     1141 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     1201 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     1261 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     1321 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     1381 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     1441 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     1501 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     1561 ctccaccggc ggcatggacg agctgtacaa gggtggaggc ggatcaggag gcggtggatc
     1621 tctcgagatg tggccccaac cccgcctccc tccccgcccc gcgatgtcgg aagaaacccg
     1681 acagagcaaa ttggccgcag cgaagaaaaa gttgagagaa tatcagcaga ggaatagccc
     1741 tggtgttcct acaggagcga aaaagaagaa gaaaataaaa aatggcagta accctgagac
     1801 aaccacttct ggtggttgcc actcacctga ggatattcag gacattctga aggtgctggt
     1861 gtccgacctt aaccgttcca atggggtagc gctcccccca ttggacaagt ggaagacacc
     1921 caaggacaat gctgctactc tacaaccatc tgatgacacc gtgttacctg gcggtgtccc
     1981 ttcccctggt gccagtctca ctagcatggc ggcatctcag aatcatgatg ctgacaatgt
     2041 ccctaatctc atggatgaaa ccaagacttt ctcatcaacc gagagcctgc gacaactctc
     2101 ccaacagctc aatggtcttg tttgtgagtc tgcgacatgt gtcaatgggg agggccctgc
     2161 atcgtctgct aacctgaagg atctggagag ccggtaccaa cagctagcgg tagccctgga
     2221 ctccagctat gtaacaaaca aacaactcaa tatcacgata gagaaattga aacaacagaa
     2281 ccaagaaatt acggatcagt tggaagaaga aaagaaagaa tgccaccaaa agcagggagc
     2341 cctaagggag cagttacagg ttcacattca gaccataggg atcctcgtat cagagaaagc
     2401 tgagttacag acagccctgg ctcacactca gcatgctgcc aggcagaaag aaggagagtc
     2461 tgaagatctg gccagccgcc tgcagtattc ccggcggcgt gtgggagagt tggagcgggc
     2521 tctctctgct gtctccacgc agcagaagaa ggcagacagg tacaacaagg agttaaccaa
     2581 agagagagac gccctcaggc tggagttata caagaacacc caaagcaatg aggacctgaa
     2641 gcaagagaaa tcagaattgg aagagaagct tcgggtccta gtgactgaga aggctggcat
     2701 gcagcttaac ttggaagaat tgcaaaagaa gttagagatg acggaactcc tgcttcaaca
     2761 gttttcaagc cggtgtgaag cccctgatgc taaccagcag ttacagcagg ccatggagga
     2821 gcgggcacag ctggaagcac acctggggca ggtaatggag tcggttagac aactacaaat
     2881 ggagagagat aaatatgcgg agaatctcaa aggagagagc gccatgtggc ggcagaggat
     2941 gcagcagatg tcagagcagg tgcacacatt gagagaggag aaggaatgta gcatgagtcg
     3001 ggtacaggag ctggagacga gcttggctga actgaggaac cagatggctg aacccccgcc
     3061 cccagagccc ccagcagggc cctccgaggt ggagcagcag ctacaagcgg aggctgagca
     3121 cctgcggaag gagctggagg gtctggcagg acagcttcaa gcccaggtgc aagacaatga
     3181 gggcttgagt cgcctgaacc gggagcagga ggagaggctg ctggagctgg agcgggcggc
     3241 cgagctctgg ggggagcagg cggaggcgcg caggcaaatc ctggagacca tgcagaacga
     3301 ccgcactacc atcagccgcg cactctccca gaaccgggag ctcaaggagc agctggctga
     3361 gctgcagagc ggatttgtaa agctgactaa tgagaacatg gagatcacca gcgcactgca
     3421 gtcggagcag cacgtcaaga gggagctggg aaagaagctg ggcgagctgc aggagaagct
     3481 gagcgagctg aaggaaacgg tggagctgaa gagccaagag gctcaaagtc tgcagcagca
     3541 gcgagaccag tacctgggac acctgcagca gtatgtggcc gcctatcagc agctgacctc
     3601 tgagaaggag gtgctgcata atcagctact gctgcagacc cagctcgtgg accagctgca
     3661 gcagcaggaa gctcagggca aagcggtggc cgagatggcc cgccaagagt tgcaggaaac
     3721 ccaggagcgc ctggaagctg ccacccagca gaatcagcag ctacgggccc agttgagcct
     3781 catggctcac cctggggaag gagatggact ggaccgggag gaggaggagg atgaggagga
     3841 ggaggaggag gaggcggtgg cagtacctca gcccatgcca agcatcccgg aggacctgga
     3901 gagccgggaa gccatggtgg catttttcaa ctcagctgta gccagtgccg aggaggagca
     3961 ggcaaggcta cgtgggcagc tgaaggagca aagggtgcgc tgccggcgcc tggctcacct
     4021 gctggcctcg gcccagaagg agcctgaggc agcagcccca gccccaggga ccgggggtga
     4081 ttctgtgtgt ggggagaccc accgggccct gcagggggcc atggagaagc tgcagagccg
     4141 ctttatggag ctcatgcagg agaaggcaga cctgaaggag agggtagagg aactggaaca
     4201 tcgctgcatc cagctttctg gagagacaga caccattgga gagtacattg cactgtacca
     4261 gagccagagg gcagtgctga aggagcggca ccgggagaag gaggagtaca tcagcaggct
     4321 ggcccaagac aaggaggaga tgaaggtgaa gctgctggag ctgcaggagc tggtcttacg
     4381 gcttgtgggc gaccgcaacg agtggcatgg cagattcctg gcagctgccc agaaccctgc
     4441 tgatgagccc acttcagggg ccccagcccc ccaggaactt ggggctgcca accagcaggg
     4501 tgatctttgc gaggtgagcc tcgccggcag tgtggagcct gcccaaggag aggccaggga
     4561 gggttctccc cgtgacaacc ccactgcaca gcagatcatg cagctgcttc gtgagatgca
     4621 gaacccccgg gagcgcccag gcttgggcag caacccctgc attccttttt tttaccgggc
     4681 tgacgagaat gatgaggtga agatcactgt catctaagga tcctctagag ggcccgttta
     4741 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4801 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4861 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4921 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4981 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     5041 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     5101 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     5161 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     5221 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     5281 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5341 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5401 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     5461 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     5521 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     5581 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     5641 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5701 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     5761 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     5821 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     5881 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     5941 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     6001 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     6061 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     6121 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     6181 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     6241 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     6301 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     6361 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     6421 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     6481 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     6541 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     6601 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     6661 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     6721 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     6781 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     6841 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6901 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6961 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     7021 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7081 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7141 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7201 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7261 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7321 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7381 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7441 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7501 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7561 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7621 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7681 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7741 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7801 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7861 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7921 gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7981 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8041 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8101 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8161 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8221 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8281 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8341 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8401 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8461 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8521 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8581 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8641 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8701 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8761 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8821 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8881 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8941 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9001 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9061 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9121 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     9181 cgatccccta tggt
//
