LOCUS       V037458                 7240 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037458
VERSION     V037458
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..2676
                     /label="GNL3L(NM_001184819)"
                     /note="GNL3L(NM_001184819)"
                     /gene="GNL3L"
     CDS             2683..2772
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     polyA_signal    2807..3031
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3077..3505
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3519..3848
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3915..4709
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4883..5004
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5053..5069)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5077..5093
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5101..5131)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5146..5167
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5455..6040)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6211..7071)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7072..7176)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atgatgaaac ttagacacaa aaataaaaag
      961 ccaggtgaag gttccaaggg ccacaagaag ataagttggc cctaccctca gcctgcaaag
     1021 caaaatggga agaaagcaac ctccaaagtg ccctctgcac ctcattttgt tcaccccaat
     1081 gatcatgcca atcgagaggc tgaattaaag aagaagtggg ttgaggagat gagggagaag
     1141 cagcaagccg cccgggagca agaaagacaa aaacgcagga ccattgagag ctactgtcag
     1201 gatgtcctaa gacgccagga ggagtttgag cataaggagg aagttttgca ggaattaaat
     1261 atgtttcctc agctggatga cgaggccacg aggaaggctt attacaagga gttccgtaag
     1321 gtggtggaat actctgatgt gattctggaa gtcctggatg ccagagaccc attaggctgc
     1381 cgctgcttcc aaatggagga ggctgtcctg cgagcacaag gcaacaagaa gctggtcctg
     1441 gtcttgaaca agattgacct ggtccccaag gaggttgtgg agaaatggct ggattacctt
     1501 cggaatgagt tgccaaccgt ggctttcaag gccagtaccc agcatcaggt caaaaacctg
     1561 aatcgttgca gtgtgccagt agatcaggcc tctgagtcac tgctgaaaag caaagcctgc
     1621 tttggagctg aaaacctcat gagggttctg gggaactatt gccgccttgg tgaagtgcgc
     1681 acccacattc gtgtgggtgt tgtgggtctt cccaatgttg ggaagagcag cctgatcaat
     1741 agcctgaagc gcagccgcgc atgcagcgtg ggagctgttc ctggaattac caaattcatg
     1801 caggaggtct acctggacaa gttcatccgg ctcttggatg ctccaggcat tgtcccaggg
     1861 cccaactcag aggtgggcac catcctgcgt aactgcgtcc acgtgcagaa gctggcagac
     1921 cctgtgaccc cagtggagac catcctgcag cgctgcaacc tggaggagat ttccaactat
     1981 tatggcgtct ctgggttcca gaccactgag cactttctga cggcagtggc ccaccgtttg
     2041 gggaagaaga agaagggagg cttatatagt caggaacagg cggccaaagc tgtcctagct
     2101 gactgggtga gcgggaagat cagcttctat ataccaccac cagccactca cactctgccc
     2161 acccatctca gtgctgagat cgttaaggaa atgaccgagg tctttgacat cgaggatact
     2221 gagcaggcca atgaagacac catggaatgc ttggccaccg gagaatctga tgagctgttg
     2281 ggtgacacgg acccacttga aatggagatc aagttgctcc attctccgat gacgaaaata
     2341 gcagatgcca ttgaaaataa aaccaccgtg tataagattg gagatctcac tgggtattgc
     2401 accaatccga accgtcatca gatggggtgg gctaaacgca atgtggacca ccgccctaag
     2461 agcaacagta tggtggatgt ctgctcagtg gaccgccgct cagtgctgca gaggatcatg
     2521 gagacggacc ccctgcaaca gggccaggct ctggcatctg ccctgaaaaa taagaagaag
     2581 atgcagaaac gtgcagataa aatcgccagc aagctgtctg attccatgat gtctgctctc
     2641 gacctctctg gcaatgctga tgatggtgtt ggtgacctcg aggaacaaaa actaatatca
     2701 gaggaagatt tggaacagaa actaatcagt gaagaagatt tagagcaaaa gttaatttcc
     2761 gaggaggact tatgagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     2821 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     2881 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     2941 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     3001 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     3061 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3121 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3181 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3241 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3301 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3361 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     3421 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     3481 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     3541 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3601 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3661 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3721 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3781 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     3841 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     3901 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     3961 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4021 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4081 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4141 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4201 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4261 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4321 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4381 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     4441 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     4501 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     4561 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     4621 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     4681 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     4741 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     4801 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     4861 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     4921 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     4981 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     5041 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5101 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5161 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5221 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5281 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5341 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5401 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5461 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5521 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5581 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5641 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5701 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5761 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5821 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5881 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5941 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt
     6001 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6061 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6121 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6181 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6241 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6301 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6361 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6421 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6481 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6541 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6601 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6661 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6721 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6781 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6841 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6901 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6961 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7021 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7081 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7141 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7201 tgacgtcgac ggatcgggag atctcccgat cccctatggt
//
