LOCUS       pcDNA3.1-GLG1(human)    8996 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039261
VERSION     V039261
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..4516
                     /label="GLG1(NM_012201)"
                     /note="GLG1(NM_012201)"
                     /gene="GLG1"
     polyA_signal    4563..4787
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4833..5261
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5275..5604
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5671..6465
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6639..6760
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6809..6825)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6833..6849
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6857..6887)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6902..6923
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7211..7796)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7967..8827)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8828..8932)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gcggcgtgtg gacgtgtacg gaggatgttc cgcttgtcgg cggcgctgca
      961 tctgctgctg ctattcgcgg ccggggccga gaaactcccc ggccagggcg tccacagcca
     1021 gggccagggt cccggggcca actttgtgtc cttcgtaggg caggccggag gcggcggccc
     1081 ggcgggtcag cagctgcccc agctgcctca gtcatcgcag cttcagcagc aacagcagca
     1141 gcagcaacag caacagcagc ctcagccgcc gcagccgcct ttcccggcgg gtgggcctcc
     1201 ggcccggcgg ggaggagcgg gggctggtgg gggctggaag ctggcggagg aagagtcctg
     1261 cagggaggac gtgacccgcg tgtgccctaa gcacacctgg agcaacaacc tggcggtgct
     1321 cgagtgcctg caggatgtga gggagcctga aaatgaaatt tcttcagact gcaatcattt
     1381 gttgtggaat tataagctga acctaactac agatcccaaa tttgaatctg tggccagaga
     1441 ggtttgcaaa tctactataa cagagattaa agaatgtgct gatgaaccgg ttggaaaagg
     1501 ttacatggtt tcctgcttgg tggatcaccg aggcaacatc actgagtatc agtgtcacca
     1561 gtacattacc aagatgacgg ccatcatttt tagtgattac cgtttaatct gtggcttcat
     1621 ggatgactgc aaaaatgaca tcaacattct gaaatgtggc agtattcggc ttggagaaaa
     1681 ggatgcacat tcacaaggtg aggtggtatc atgcttggag aaaggcctgg tgaaagaagc
     1741 agaagaaaga gaacccaaga ttcaagtttc tgaactctgc aagaaagcca ttctccgggt
     1801 ggctgagctg tcatcggatg actttcactt agaccggcat ttatattttg cttgccgaga
     1861 tgatcgggag cgtttttgtg aaaatacaca agctggtgag ggcagagtgt ataagtgcct
     1921 ctttaaccat aaatttgaag aatccatgag tgaaaagtgt cgagaagcac ttacaacccg
     1981 ccaaaagctg attgcccagg attataaagt cagttattca ttggccaaat cctgtaaaag
     2041 tgacttgaag aaataccggt gcaatgtgga aaaccttccg cgatcgcgtg aagccaggct
     2101 ctcctacttg ttaatgtgcc tggagtcagc tgtacacaga gggcgacaag tcagcagtga
     2161 gtgccagggg gagatgctgg attaccgacg catgttgatg gaagactttt ctctgagccc
     2221 tgagatcatc ctaagctgtc ggggggagat tgaacaccat tgttccggat tacatcgaaa
     2281 agggcggacc ctacactgtc tgatgaaagt agttcgaggg gagaagggga accttggaat
     2341 gaactgccag caggcgcttc aaacactgat tcaggagact gaccctggtg cagattaccg
     2401 cattgatcga gctttgaatg aagcttgtga atctgtaatc cagacagcct gcaaacatat
     2461 aagatctgga gacccaatga tcttgtcgtg cctgatggaa catttataca cagagaagat
     2521 ggtagaagac tgtgaacacc gtctcttaga gctgcagtat ttcatctccc gggattggaa
     2581 gctggaccct gtcctgtacc gcaagtgcca gggagacgct tctcgtcttt gccacaccca
     2641 cggttggaat gagaccagtg aatttatgcc tcagggagct gtgttctctt gtttatacag
     2701 acacgcctac cgcactgagg aacagggaag gaggctctca cgggagtgcc gagctgaagt
     2761 ccaaaggatc ctacaccagc gtgccatgga tgtcaagctg gatcctgccc tccaggataa
     2821 gtgcctgatt gatctgggaa aatggtgcag tgagaaaaca gagactggac aggagctgga
     2881 gtgccttcag gaccatctgg atgacttggt ggtggagtgt agagatatag ttggcaacct
     2941 cactgagtta gaatcagagg atattcaaat agaagccttg ctgatgagag cctgtgagcc
     3001 cataattcag aacttctgcc acgatgtggc agataaccag atagactctg gggacctgat
     3061 ggagtgtctg atacagaaca aacaccagaa ggacatgaac gagaagtgtg ccatcggagt
     3121 tacccacttc cagctggtgc agatgaagga ttttcggttt tcttacaagt ttaaaatggc
     3181 ctgcaaggag gacgtgttga agctttgccc aaacataaaa aagaaggtgg acgtggtgat
     3241 ctgcctgagc acgaccgtgc gcaatgacac tctgcaggaa gccaaggagc acagggtgtc
     3301 cctgaagtgc cgcaggcagc tccgtgtgga ggagctggag atgacggagg acatccgctt
     3361 ggagccagat ctatacgaag cctgcaagag tgacatcaaa aacttctgtt ccgctgtgca
     3421 atatggcaac gctcagatta tcgaatgtct gaaagaaaac aagaagcagc taagcacccg
     3481 ctgccaccaa aaagtattta agctgcagga gacagagatg atggacccag agctagacta
     3541 caccctcatg agggtctgca agcagatgat aaagaggttc tgtccggaag cagattctaa
     3601 aaccatgttg cagtgcttga agcaaaataa aaacagtgaa ttgatggatc ccaaatgcaa
     3661 acagatgata accaagcgcc agatcaccca gaacacagat taccgcttaa accccatgtt
     3721 aagaaaagcc tgtaaagctg acattcctaa attctgtcac ggtatcctga ctaaggccaa
     3781 ggatgattca gaattagaag gacaagtcat ctcttgcctg aagctgagat atgctgacca
     3841 gcgcctgtct tcagactgtg aagaccagat ccgaatcatt atccaggagt ccgccctgga
     3901 ctaccgcctg gatcctcagc tccagctgca ctgctcagac gagatctcca gtctatgtgc
     3961 tgaagaagca gcagcccaag agcagacagg tcaggtggag gagtgcctca aggtcaacct
     4021 gctcaagatc aaaacagaat tgtgtaaaaa ggaagtgcta aacatgctga aggaaagcaa
     4081 agcagacatc tttgttgacc cggtacttca tactgcttgt gccctggaca ttaaacacca
     4141 ctgcgcagcc atcacccctg gccgcgggcg tcaaatgtcc tgtctcatgg aagcactgga
     4201 ggataagcgg gtgaggttac agcccgagtg caaaaagcgc ctcaatgacc ggattgagat
     4261 gtggagttac gcagcaaagg tggccccagc agatggcttc tctgatcttg ccatgcaagt
     4321 aatgacgtct ccatctaaga actacattct ctctgtgatc agtgggagca tctgtatatt
     4381 gttcctgatt ggcctgatgt gtggacggat caccaagcga gtgacacgag agctcaagga
     4441 caggctacaa tacaggtcag agacaatggc ttataaaggt ttagtgtggt ctcaggatgt
     4501 gacaggcagt ccagcctagc tcgagtctag agggcccgtt taaacccgct gatcagcctc
     4561 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     4621 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     4681 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     4741 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     4801 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     4861 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4921 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4981 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5041 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5101 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5161 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5221 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     5281 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5341 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5401 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5461 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5521 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     5581 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     5641 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     5701 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     5761 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     5821 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     5881 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     5941 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     6001 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     6061 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     6121 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     6181 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     6241 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     6301 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     6361 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     6421 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     6481 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     6541 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     6601 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     6661 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6721 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     6781 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6841 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6901 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6961 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7021 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7081 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7141 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7201 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7261 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7321 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7381 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7441 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7501 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7561 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7621 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7681 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7741 gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7801 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7861 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7921 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7981 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8041 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8101 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8161 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8221 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8281 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8341 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8401 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8461 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8521 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8581 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8641 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8701 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8761 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8821 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8881 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8941 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggt
//
