LOCUS       V024486                 7978 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024486
VERSION     V024486
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             989..3544
                     /label="GANAB(NM_001278192)"
                     /note="GANAB(NM_001278192)"
                     /gene="GANAB"
     polyA_signal    3578..3802
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3848..4276
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4290..4619
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4686..5480
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5654..5775
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5824..5840)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5848..5864
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5872..5902)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5917..5938
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6226..6811)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6982..7842)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7843..7947)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcc
      961 accacactgg actagtggat ccgccaccat ggcggcggta gcggcagtgg cggcgcgtag
     1021 gaggcggctt tctgtctctg gtcgtgatga gaacagtgtg gagttaacca tggctgaggg
     1081 accctacaag atcatcttga cagcacggcc attccgcctt gacctactag aggaccgaag
     1141 tcttttgctt agtgtcaatg cccgaggact cttggagttt gagcatcaga gggcccctag
     1201 ggtctctttc tcggataagg ttaatctcac gcttggtagc atatgggata agatcaagaa
     1261 ccttttctct aggcaaggat caaaagaccc agctgagggc gatggggccc agcctgagga
     1321 aacacccagg gatggcgaca agccagagga gactcagggg aaggcagaga aagatgagcc
     1381 aggagcctgg gaggagacat tcaaaactca ctctgacagc aagccgtatg gccccatgtc
     1441 tgtgggtttg gacttctctc tgccaggcat ggagcatgtc tatgggatcc ctgagcatgc
     1501 agacaacctg aggctgaagg tcactgaggg tggggagcca tatcgcctct acaatttgga
     1561 tgtgttccag tatgagctgt acaacccaat ggccttgtat gggtctgtgc ctgtgctcct
     1621 ggcacacaac cctcatcgcg acttgggcat cttctggctc aatgctgcag agacctgggt
     1681 tgatatatct tccaacactg ccgggaagac cctgtttggg aagatgatgg actacctgca
     1741 gggctctggg gagaccccac agacagatgt tcgctggatg tcagagactg gcatcattga
     1801 cgtcttcctg ctgctggggc cctccatctc tgatgttttc cggcaatatg ctagtctcac
     1861 aggaacccag gcgttgcccc cactcttctc cctcggctac caccagagcc gttggaacta
     1921 ccgggacgag gctgatgtgc tggaagtgga tcagggcttt gatgatcaca acctgccctg
     1981 tgatgtcatc tggctagaca ttgaacatgc tgatggcaag cggtatttca cctgggaccc
     2041 cagtcgcttc cctcagcccc gcaccatgct tgagcgcttg gcttctaaga ggcggaagct
     2101 ggtggccatc gtagaccccc acatcaaggt ggactccggc taccgagttc acgaggagct
     2161 gcggaacctg gggctgtatg ttaaaacccg ggatggctct gactatgagg gctggtgctg
     2221 gccaggctca gctggttacc ctgacttcac taatcccacg atgagggcct ggtgggctaa
     2281 catgttcagc tatgacaatt atgagggctc agctcccaac ctctttgtct ggaatgacat
     2341 gaacgaacca tctgtgttca atggtcctga ggtcaccatg ctcaaggatg cccagcatta
     2401 tgggggctgg gagcaccggg atgtgcataa catctatggc ctttatgtgc acatggcgac
     2461 tgctgatggg ctgagacagc gctctggggg catggaacgc ccctttgtcc tggccagggc
     2521 cttcttcgct ggctcccagc gctttggagc cgtgtggaca ggggacaaca ctgccgagtg
     2581 ggaccatttg aagatctcta ttcctatgtg tctcagcttg gggctggtgg gactttcctt
     2641 ctgtggggcg gatgtgggtg gcttcttcaa aaacccagag ccagagctgc ttgtgcgctg
     2701 gtaccagatg ggtgcttacc agccattctt ccgggcacat gcccacttgg acactgggcg
     2761 acgagagcca tggctgttac catctcagca caatgatata atccgagatg ccttgggcca
     2821 gcgatattct ttgctgccct tctggtacac cctcttatat caggcccatc gggaaggcat
     2881 tcctgtcatg aggcccctgt gggtgcagta ccctcaggat gtgactacct tcaatataga
     2941 tgatcagtac ttgcttgggg atgcgttgct ggttcaccct gtatcagact ctggagccca
     3001 tggtgtccag gtctatctgc ctggccaagg ggaggtgtgg tatgacattc aaagctacca
     3061 gaagcatcat ggtccccaga ccctgtacct gcctgtaact ctaagcagta tccctgtgtt
     3121 ccagcgtgga gggacaatcg tgcctcgatg gatgcgagtg cggcggtctt cagaatgtat
     3181 gaaggatgac cccatcactc tctttgttgc acttagccct cagggtacag ctcaaggaga
     3241 gctctttctg gatgatgggc acacgttcaa ctatcagact cgccaagagt tcctgctgcg
     3301 tcgattctca ttctctggca acacccttgt ctccagctca gcagaccctg aaggacactt
     3361 tgagacacca atctggattg agcgggtggt gataataggg gctggaaagc cagcagctgt
     3421 ggtactccag acaaaaggat ctccagaaag ccgcctgtcc ttccagcatg accctgagac
     3481 ctctgtgttg gtcctgcgca agcctggcat caatgtggca tctgattgga gtattcacct
     3541 gcgataagct taagtttaaa ccgctgatca gcctcgactg tgccttctag ttgccagcca
     3601 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3661 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3721 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     3781 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     3841 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3901 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     3961 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4021 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4081 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4141 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4201 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4261 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     4321 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4381 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4441 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4501 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     4561 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     4621 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     4681 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     4741 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     4801 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     4861 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     4921 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     4981 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     5041 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     5101 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     5161 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     5221 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     5281 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     5341 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     5401 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     5461 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     5521 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     5581 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     5641 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5701 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5761 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     5821 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     5881 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     5941 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6001 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6061 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6121 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6181 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6241 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6301 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6361 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6421 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6481 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6541 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6601 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6661 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6721 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtttttttg tttgcaagca
     6781 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6841 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6901 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6961 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7021 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7081 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7141 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7201 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7261 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     7321 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     7381 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     7441 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     7501 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7561 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     7621 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7681 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7741 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7801 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7861 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7921 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
