LOCUS       V020718                 7833 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020718
VERSION     V020718
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3289
                     /label="FURIN(NM_001289823)"
                     /note="FURIN(NM_001289823)"
                     /gene="FURIN"
     CDS             3305..3322
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     polyA_signal    3400..3624
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3670..4098
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4112..4441
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4508..5302
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5476..5597
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5646..5662)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5670..5686
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5694..5724)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5739..5760
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6048..6633)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6804..7664)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7665..7769)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gagctgaggc cctggttgct atgggtggta gcagcaacag gaaccttggt
      961 cctgctagca gctgatgctc agggccagaa ggtcttcacc aacacgtggg ctgtgcgcat
     1021 ccctggaggc ccagcggtgg ccaacagtgt ggcacggaag catgggttcc tcaacctggg
     1081 ccagatcttc ggggactatt accacttctg gcatcgagga gtgacgaagc ggtccctgtc
     1141 gcctcaccgc ccgcggcaca gccggctgca gagggagcct caagtacagt ggctggaaca
     1201 gcaggtggca aagcgacgga ctaaacggga cgtgtaccag gagcccacag accccaagtt
     1261 tcctcagcag tggtacctgt ctggtgtcac tcagcgggac ctgaatgtga aggcggcctg
     1321 ggcgcagggc tacacagggc acggcattgt ggtctccatt ctggacgatg gcatcgagaa
     1381 gaaccacccg gacttggcag gcaattatga tcctggggcc agttttgatg tcaatgacca
     1441 ggaccctgac ccccagcctc ggtacacaca gatgaatgac aacaggcacg gcacacggtg
     1501 tgcgggggaa gtggctgcgg tggccaacaa cggtgtctgt ggtgtaggtg tggcctacaa
     1561 cgcccgcatt ggaggggtgc gcatgctgga tggcgaggtg acagatgcag tggaggcacg
     1621 ctcgctgggc ctgaacccca accacatcca catctacagt gccagctggg gccccgagga
     1681 tgacggcaag acagtggatg ggccagcccg cctcgccgag gaggccttct tccgtggggt
     1741 tagccagggc cgaggggggc tgggctccat ctttgtctgg gcctcgggga acgggggccg
     1801 ggaacatgac agctgcaact gcgacggcta caccaacagt atctacacgc tgtccatcag
     1861 cagcgccacg cagtttggca acgtgccgtg gtacagcgag gcctgctcgt ccacactggc
     1921 cacgacctac agcagtggca accagaatga gaagcagatc gtgacgactg acttgcggca
     1981 gaagtgcacg gagtctcaca cgggcacctc agcctctgcc cccttagcag ccggcatcat
     2041 tgctctcacc ctggaggcca ataagaacct cacatggcgg gacatgcaac acctggtggt
     2101 acagacctcg aagccagccc acctcaatgc caacgactgg gccaccaatg gtgtgggccg
     2161 gaaagtgagc cactcatatg gctacgggct tttggacgca ggcgccatgg tggccctggc
     2221 ccagaattgg accacagtgg ccccccagcg gaagtgcatc atcgacatcc tcaccgagcc
     2281 caaagacatc gggaaacggc tcgaggtgcg gaagaccgtg accgcgtgcc tgggcgagcc
     2341 caaccacatc actcggctgg agcacgctca ggcgcggctc accctgtcct ataatcgccg
     2401 tggcgacctg gccatccacc tggtcagccc catgggcacc cgctccaccc tgctggcagc
     2461 caggccacat gactactccg cagatgggtt taatgactgg gccttcatga caactcattc
     2521 ctgggatgag gatccctctg gcgagtgggt cctagagatt gaaaacacca gcgaagccaa
     2581 caactatggg acgctgacca agttcaccct cgtactctat ggcaccgccc ctgaggggct
     2641 gcccgtacct ccagaaagca gtggctgcaa gaccctcacg tccagtcagg cctgtgtggt
     2701 gtgcgaggaa ggcttctccc tgcaccagaa gagctgtgtc cagcactgcc ctccagggtt
     2761 cgccccccaa gtcctcgata cgcactatag caccgagaat gacgtggaga ccatccgggc
     2821 cagcgtctgc gccccctgcc acgcctcatg tgccacatgc caggggccgg ccctgacaga
     2881 ctgcctcagc tgccccagcc acgcctcctt ggaccctgtg gagcagactt gctcccggca
     2941 aagccagagc agccgagagt ccccgccaca gcagcagcca cctcggctgc ccccggaggt
     3001 ggaggcgggg caacggctgc gggcagggct gctgccctca cacctgcctg aggtggtggc
     3061 cggcctcagc tgcgccttca tcgtgctggt cttcgtcact gtcttcctgg tcctgcagct
     3121 gcgctctggc tttagttttc ggggggtgaa ggtgtacacc atggaccgtg gcctcatctc
     3181 ctacaagggg ctgccccctg aagcctggca ggaggagtgc ccgtctgact cagaagagga
     3241 cgagggccgg ggcgagagga ccgcctttat caaagaccag agcgccctcg gcggtggagg
     3301 aagtcatcac catcaccatc actgaattct gcagatatcc agcacagtgg cggccgctcg
     3361 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     3421 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3481 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3541 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     3601 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     3661 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3721 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     3781 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     3841 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     3901 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     3961 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4021 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4081 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     4141 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     4201 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4261 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4321 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4381 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     4441 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     4501 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     4561 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     4621 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     4681 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     4741 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     4801 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     4861 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     4921 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     4981 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     5041 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     5101 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     5161 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     5221 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     5281 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     5341 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     5401 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     5461 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5521 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5581 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     5641 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     5701 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     5761 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     5821 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     5881 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5941 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6001 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6061 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6121 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6181 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6241 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6301 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6361 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6421 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6481 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6541 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag
     6601 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6661 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6721 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6781 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6841 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6901 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6961 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7021 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7081 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7141 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7201 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7261 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7321 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7381 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7441 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7501 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7561 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7621 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7681 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7741 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     7801 gacggatcgg gagatctccc gatcccctat ggt
//
