LOCUS V043653 7504 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V043653 VERSION V043653 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 964..2994 /label="FOXP1(NM_032682)" /note="FOXP1(NM_032682)" /gene="FOXP1" CDS 3001..3066 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 3104..3328 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3374..3802 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3816..4145 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4212..5006 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5180..5301 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5350..5366) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5374..5390 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5398..5428) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5443..5464 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5752..6337) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6508..7368) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7369..7473) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc 961 accatgatgc aagaatctgg gactgagaca aaaagtaacg gttcagccat ccagaatggg 1021 tcgggcggca gcaaccactt actagagtgc ggcggtcttc gggaggggcg gtccaacgga 1081 gagacgccgg ccgtggacat cggggcagct gacctcgccc acgcccagca gcagcagcaa 1141 caggcacttc aggtggcaag acagctcctt cttcagcagc aacagcagca gcaagttagt 1201 ggattaaaat ctcccaagag gaatgacaaa caaccagctc ttcaggttcc cgtgtcagtg 1261 gctatgatga cacctcaagt tatcactccc cagcaaatgc agcagatcct ccagcaacaa 1321 gtgctgagcc ctcagcagct ccaggttctc ctccagcagc agcaggccct catgcttcaa 1381 cagcagcagc ttcaagagtt ttataaaaaa caacaggaac agttgcagct tcaactttta 1441 caacaacaac atgctggaaa acagcctaaa gagcaacagc aggtggctac ccagcagttg 1501 gcttttcagc agcagctttt acagatgcag cagttacagc agcagcacct cctgtctttg 1561 cagcgccaag gccttctgac aattcagccc gggcagcctg cccttcccct tcaacctctt 1621 gctcaaggca tgattccaac agaactgcag cagctctgga aagaagtgac aagtgctcat 1681 actgcagaag aaaccacagg caacaatcac agcagtttgg atctgaccac gacatgtgtc 1741 tcctcctctg caccttccaa gacctcctta ataatgaacc cacatgcctc taccaatgga 1801 cagctctcag tccacactcc caaaagggaa agtttgtccc atgaggagca cccccatagc 1861 catcctctct atggacatgg tgtatgcaag tggccaggct gtgaagcagt gtgcgaagat 1921 ttccaatcat ttctaaaaca tctcaacagt gagcatgcgc tggacgatag aagtacagcc 1981 caatgtagag tacaaatgca ggttgtacag cagttagagc tacagcttgc aaaagacaaa 2041 gaacgcctgc aagccatgat gacccacctg catgtgaagt ctacagaacc caaagccgcc 2101 cctcagccct tgaatctggt atcaagtgtc actctctcca agtccgcatc ggaggcttct 2161 ccacagagct tacctcatac tccaacgacc ccaaccgccc ccctgactcc cgtcacccaa 2221 ggcccctctg tcatcacaac caccagcatg cacacggtgg gacccatccg caggcggtac 2281 tcagacaaat acaacgtgcc catttcgtca gcagatattg cgcagaacca agaattttat 2341 aagaacgcag aagttagacc accatttaca tatgcatctt taattaggca ggccattctc 2401 gaatctccag aaaagcagct aacactaaat gagatctata actggttcac acgaatgttt 2461 gcttacttcc gacgcaacgc ggccacgtgg aagaatgcag tgcgtcataa tcttagtctt 2521 cacaagtgtt ttgtgcgagt agaaaacgtt aaaggggcag tatggacagt ggatgaagta 2581 gaattccaaa aacgaaggcc acaaaagatc agtggtaacc cttcccttat taaaaacatg 2641 cagagcagcc acgcctactg cacacctctc aatgcagctt tacaggcttc aatggctgag 2701 aatagtatac ctctatacac taccgcttcc atgggaaatc ccactctggg caacttagcc 2761 agcgcaatac gggaagagct gaacggggca atggagcata ccaacagcaa cgagagtgac 2821 agcagtccag gcagatctcc tatgcaagcc gtgcatcctg tacacgtcaa agaagagccc 2881 ctcgatccag aggaagctga agggcccctg tccttagtga caacagccaa ccacagtcca 2941 gattttgacc atgacagaga ttacgaagat gaaccagtaa acgaggacat ggagctcgag 3001 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat 3061 gacaagtagt gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc 3121 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc 3181 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct 3241 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg 3301 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct 3361 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 3421 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 3481 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 3541 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 3601 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 3661 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 3721 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt 3781 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt 3841 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 3901 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 3961 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 4021 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 4081 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 4141 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag 4201 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 4261 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 4321 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 4381 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 4441 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 4501 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 4561 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 4621 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 4681 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc 4741 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 4801 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 4861 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 4921 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 4981 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc 5041 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg 5101 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct 5161 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa 5221 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 5281 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc 5341 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 5401 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 5461 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 5521 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 5581 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 5641 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 5701 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 5761 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 5821 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 5881 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 5941 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 6001 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 6061 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 6121 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 6181 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 6241 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg 6301 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 6361 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 6421 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 6481 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 6541 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 6601 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 6661 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 6721 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 6781 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 6841 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 6901 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 6961 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 7021 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 7081 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 7141 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 7201 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 7261 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 7321 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 7381 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 7441 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 7501 cgtc //