LOCUS V022340 7436 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V022340 VERSION V022340 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..2959 /label="FOXO3(NM_001455)" /note="FOXO3(NM_001455)" /gene="FOXO3" CDS 2975..2998 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" polyA_signal 3036..3260 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3306..3734 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3748..4077 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4144..4938 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5112..5233 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5282..5298) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5306..5322 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5330..5360) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5375..5396 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5684..6269) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6440..7300) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7301..7405) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggcagagg caccggcttc 961 cccggccccg ctctctccgc tcgaagtgga gctggacccg gagttcgagc cccagagccg 1021 tccgcgatcc tgtacgtggc ccctgcaaag gccggagctc caagcgagcc ctgccaagcc 1081 ctcgggggag acggccgccg actccatgat ccccgaggag gaggacgatg aagacgacga 1141 ggacggcggg ggacgggccg gctcggccat ggcgatcggc ggcggcggcg ggagcggcac 1201 gctgggctcc gggctgctcc ttgaggactc ggcccgggtg ctggcacccg gagggcaaga 1261 ccccgggtct gggccagcca ccgcggcggg cgggctgagc gggggtacac aggcgctgct 1321 gcagcctcag caaccgctgc caccgccgca gccgggggcg gctgggggct ccgggcagcc 1381 gaggaaatgt tcgtcgcggc ggaacgcctg gggaaacctg tcctacgcgg acctgatcac 1441 ccgcgccatc gagagctccc cggacaaacg gctcactctg tcccagatct acgagtggat 1501 ggtgcgttgc gtgccctact tcaaggataa gggcgacagc aacagctctg ccggctggaa 1561 gaactccatc cggcacaacc tgtcactgca tagtcgattc atgcgggtcc agaatgaggg 1621 aactggcaag agctcttggt ggatcatcaa ccctgatggg gggaagagcg gaaaagcccc 1681 ccggcggcgg gctgtctcca tggacaatag caacaagtat accaagagcc gtggccgcgc 1741 agccaagaag aaggcagccc tgcagacagc ccccgaatca gctgacgaca gtccctccca 1801 gctctccaag tggcctggca gccccacgtc acgcagcagt gatgagctgg atgcgtggac 1861 ggacttccgt tcacgcacca attctaacgc cagcacagtc agtggccgcc tgtcgcccat 1921 catggcaagc acagagttgg atgaagtcca ggacgatgat gcgcctctct cgcccatgct 1981 ctacagcagc tcagccagcc tgtcaccttc agtaagcaag ccgtgcacgg tggaactgcc 2041 acggctgact gatatggcag gcaccatgaa tctgaatgat gggctgactg aaaacctcat 2101 ggacgacctg ctggataaca tcacgctccc gccatcccag ccatcgccca ctgggggact 2161 catgcagcgg agctctagct tcccgtatac caccaagggc tcgggcctgg gctccccaac 2221 cagctccttt aacagcacgg tgttcggacc ttcatctctg aactccctac gccagtctcc 2281 catgcagacc atccaagaga acaagccagc taccttctct tccatgtcac actatggtaa 2341 ccagacactc caggacctgc tcacttcgga ctcacttagc cacagcgatg tcatgatgac 2401 acagtcggac cccttgatgt ctcaggccag caccgctgtg tctgcccaga attcccgccg 2461 gaacgtgatg cttcgcaatg atccgatgat gtcctttgct gcccagccta accagggaag 2521 tttggtcaat cagaacttgc tccaccacca gcaccaaacc cagggcgctc ttggtggcag 2581 ccgtgccttg tcgaattctg tcagcaacat gggcttgagt gagtccagca gccttgggtc 2641 agccaaacac cagcagcagt ctcctgtcag ccagtctatg caaaccctct cggactctct 2701 ctcaggctcc tccttgtact caactagtgc aaacctgccc gtcatgggcc atgagaagtt 2761 ccccagcgac ttggacctgg acatgttcaa tgggagcttg gaatgtgaca tggagtccat 2821 tatccgtagt gaactcatgg atgctgatgg gttggatttt aactttgatt ccctcatctc 2881 cacacagaat gttgttggtt tgaacgtggg gaacttcact ggtgctaagc aggcctcatc 2941 tcagagctgg gtgccaggct ggcggccgct cgaggattac aaggatgacg acgataagta 3001 gtgagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc 3061 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 3121 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 3181 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 3241 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta 3301 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 3361 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 3421 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 3481 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 3541 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 3601 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 3661 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 3721 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 3781 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 3841 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 3901 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 3961 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc 4021 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag 4081 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt 4141 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 4201 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 4261 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 4321 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 4381 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 4441 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 4501 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 4561 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 4621 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc 4681 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 4741 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 4801 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 4861 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 4921 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca 4981 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 5041 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 5101 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 5161 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 5221 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat 5281 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 5341 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 5401 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 5461 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 5521 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 5581 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 5641 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 5701 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 5761 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 5821 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 5881 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 5941 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 6001 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 6061 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 6121 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 6181 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc 6241 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 6301 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 6361 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 6421 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 6481 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 6541 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 6601 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 6661 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 6721 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 6781 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 6841 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 6901 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 6961 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 7021 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 7081 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 7141 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 7201 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 7261 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 7321 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 7381 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc //