LOCUS V023625 7394 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V023625 VERSION V023625 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..2884 /label="FOXN1(NM_001369369)" /note="FOXN1(NM_001369369)" /gene="FOXN1" CDS 2891..2956 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 2994..3218 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3264..3692 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3706..4035 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4102..4896 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5070..5191 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5240..5256) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5264..5280 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5288..5318) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5333..5354 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5642..6227) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6398..7258) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7259..7363) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggtgtcgc tacccccgcc 961 gcagtctgac gtcacgctgc cgggccccac cagactggag ggcgagcgcc aaggggacct 1021 catgcaggca ccgggcctcc caggctcccc tgccccacag agtaagcatg ccggcttcag 1081 ctgctcgtca tttgtgtccg acggccctcc agagaggaca ccctcactgc ccccacacag 1141 cccccgcatt gcgtcaccag ggcccgagca agtccagggc cactgcccag ccggccccgg 1201 ccctgggccc ttcaggctct caccctcaga caagtatcct ggctttggct ttgaggaggc 1261 cgcagcaagc agccctgggc gattcctcaa gggcagccac gcgcccttcc acccgtacaa 1321 gcggcctttc catgaggacg tcttcccaga ggccgagacc accctggccc tcaaaggaca 1381 ctcctttaag accccagggc cgctggaggc cttcgaggag atcccagtgg acgtggcgga 1441 ggccgaggcc ttcctgcctg gcttctcagc agaggcctgg tgtaacgggc tcccctaccc 1501 cagccaggag catggccccc aagtcctggg ttcagaggtc aaagtcaagc ccccagttct 1561 ggagagtggt gctgggatgt tctgctacca gcctcccttg cagcatatgt actgctcctc 1621 ccagcccccc ttccaccagt actcgccagg tggtggcagc taccccatac cctacctggg 1681 ctcctcacac tatcagtacc agcgaatggc accccaggcc agcaccgatg ggcaccagcc 1741 tctcttccca aaacccatct attcctacag catcctcatc ttcatggccc ttaagaacag 1801 taaaactggg agccttcccg tcagcgagat ctacaatttt atgacggagc actttcctta 1861 cttcaagaca gcacccgatg gctggaagaa ttctgtccgg cacaacctat ccctcaacaa 1921 gtgcttcgag aaggtggaga acaaatcagg aagttcctcc cgcaagggct gcctgtgggc 1981 cctcaatccg gccaagatcg acaagatgca agaggagctg caaaaatgga agaggaaaga 2041 tcccattgct gtgcgcaaaa gcatggccaa gccagaagag ctggacagcc tcattggaga 2101 caagagagaa aagctgggct ccccactcct gggctgtccg ccccctgggc tgtccggctc 2161 aggccccatc cggcccctgg cacccccagc tggcctctcc ccaccactgc actcactcca 2221 cccagctcca ggccccattc ctggcaagaa ccccctgcag gacctactta tggggcacac 2281 accctcctgc tatgggcaga catacttgca cctctcacca ggcctggccc ctcctggacc 2341 cccgcagcca ttgttcccac agccggacgg gcaccttgag ctgcgggccc agccaggcac 2401 cccccaggac tcgcctctgc ctgcccacac cccacccagc cacagtgcca agctactggc 2461 cgagccttcc ccagccagga ctatgcacga caccctgctg ccagatggag accttggcac 2521 tgacctggat gccatcaatc cctcactcac tgacttcgac ttccagggaa acctgtggga 2581 acagttgaag gatgatagct tggccctcga ccccctggta ctggtgacct catccccgac 2641 atcatcttcg atgccaccac cccagccacc acctcactgc ttcccccctg ggccctgtct 2701 gacagagaca ggcagtgggg caggtgactt ggcagccccg ggcagtggtg gctccggggc 2761 actgggtgac ctgcacctca ccaccctcta ctctgccttt atggagctgg agcccacgcc 2821 ccccacggcc cctgcaggcc cctctgtgta cctcagcccc agctccaagc ccgtggccct 2881 ggcactcgag gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa 2941 ggatgacgat gacaagtagt gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc 3001 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 3061 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 3121 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 3181 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag 3241 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 3301 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 3361 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 3421 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 3481 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 3541 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 3601 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 3661 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg 3721 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 3781 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 3841 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 3901 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 3961 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 4021 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag 4081 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 4141 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 4201 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 4261 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg 4321 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 4381 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 4441 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 4501 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 4561 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 4621 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 4681 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 4741 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 4801 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 4861 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga 4921 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg 4981 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg 5041 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca 5101 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 5161 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct 5221 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 5281 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 5341 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 5401 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 5461 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 5521 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 5581 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 5641 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 5701 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 5761 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 5821 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 5881 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 5941 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 6001 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 6061 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 6121 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt 6181 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 6241 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 6301 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 6361 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 6421 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 6481 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 6541 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 6601 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 6661 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 6721 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 6781 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 6841 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 6901 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 6961 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 7021 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 7081 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 7141 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 7201 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 7261 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 7321 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 7381 tgccacctga cgtc //