LOCUS       V034331                11615 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034331
VERSION     V034331
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             941..7105
                     /label="EVPL(NM_001320747)"
                     /note="EVPL(NM_001320747)"
                     /gene="EVPL"
     CDS             7112..7177
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    7215..7439
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      7485..7913
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7927..8256
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8323..9117
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9291..9412
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9461..9477)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9485..9501
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9509..9539)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9554..9575
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9863..10448)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10619..11479)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11480..11584)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgttcaagg ggctgagcaa
      961 aggctcccag gggaaggggt cccccaaggg ctcccccgcc aaggggtccc ccaaaggctc
     1021 ccccagcagg cacagccggg ctgccaccca ggagctggcc cttctcatct cccgcatgca
     1081 agccaacgcc gaccaggtgg agcgggacat cctggagacg cagaagaggc tgcagcagga
     1141 ccggctgaac agtgagcaga gccaggccct gcagcaccag caggagacgg gccgcagcct
     1201 gaaggaggct gaggtgctgc tcaaggacct cttcctggac gtggacaagg cccggcggct
     1261 caagcacccg caggctgagg agattgagaa ggacatcaag cagctgcacg agcgggtgac
     1321 ccaggagtgt gcggagtacc gtgccctgta cgagaagatg gtgctgcccc ccgacgtggg
     1381 acccagggtc gactgggcac gcgtgctgga gcagaaacag aagcaggtct gcgcaggcca
     1441 gtacgggccg ggcatggcgg agctggagca acagatcgcc gagcacaaca tcctgcagaa
     1501 ggagatcgac gcctatgggc agcagctgcg gagcctcgtg gggccggatg cagccaccat
     1561 ccggagccaa taccgagacc tactgaaggc ggcgtcgtgg cgcgggcaga gcctgggcag
     1621 cctgtacacg cacctccagg gctgcacgcg gcagctgagc gccctggctg agcagcagcg
     1681 ccgcatcctg cagcaggact ggagcgacct catggccgac cctgcgggcg tgcggcggga
     1741 gtacgagcac ttcaagcagc acgagctgct gagccaggag cagagcgtga accagctgga
     1801 ggacgacggc gagcgcatgg tggagctgcg gcaccccgcg gtggggccca tccaggccca
     1861 ccaggaggcc ctgaagatgg agtggcagaa cttcctgaac ctgtgtatct gccaggagac
     1921 ccagctgcag cacgtggagg actaccgccg gttccaggaa gaggccgact cagtcagcca
     1981 gaccctggcg aagctcaact ccaacttgga tgccaagtac agccctgcac ctgggggccc
     2041 ccctggcgcc cccacagagc tgctgcaaca gctggaggca gaggaaaaac ggctggccgt
     2101 caccgagagg gccactgggg acctgcagcg gcgaagccgg gatgtggccc ctctgccaca
     2161 gcgaagaaac ccccctcagc agcccctgca cgtggacagc atctgcgact gggactcagg
     2221 agaagtgcag ctgctgcagg gtgagcggta taagctggta gataacactg acccgcacgc
     2281 ctgggtcgtg cagggccctg gcggggagac caagcgtgct cccgccgcct gcttctgcat
     2341 cccagcacca gaccctgatg ctgtggccag ggcctcccgg ctggcctcag agctgcaggc
     2401 cctgaagcag aaattggcca cagtccagag ccgcctgaag gccagtgctg tggagtctct
     2461 tcggcccagc cagcagggta cccagggtgg cccggggaga ggtggctgtg gggctagaag
     2521 gagtagattc cgacttccga ctgctccatc tggctcagac ctggccaacc cacaggccca
     2581 gaagctcctg acacagatga cccggctgga tggagacctg ggacagatag agaggcaggt
     2641 gctggcctgg gcgcgggccc cgctgagccg ccccacaccc ttggaggact tggagggccg
     2701 catccacagc catgagggca cagcccagcg cctgcagagc ctgggaacgg agaaggagac
     2761 agcccagaag gagtgcgagg cgtttctgtc cacgcggccc gtgggccccg ctgccctgca
     2821 gctgcccgta gccctcaaca gcgtgaagaa caagttcagt gacgtgcagg ttctgtgcag
     2881 cctctacggg gagaaagcca aggctgccct ggatctggag cggcagatcc aggatgcgga
     2941 cagggtcatc cgaggcttcg aggccaccct ggtgcaggag gcccccatcc ctgctgaacc
     3001 gggggctctg caggagaggg tcagcgagct gcagcgccag cggagggagc tgctggaaca
     3061 gcagacctgc gtgctgcggc tacaccgcgc gctgaaggcc tcggagcacg catgcgctgc
     3121 cctgcagaac aacttccagg agttctgcca agacctgcct cgccagcagc gccaggtgcg
     3181 agccctcacc gaccgctacc acgccgtagg ggaccagctg gacctgcggg agaaggtggt
     3241 gcaggatgcc gccctcacct accagcagtt caagaactgc aaggataacc tgagctcctg
     3301 gctggagcac ctgccccgca gccaggtgcg gcccagcgac ggccccagcc agatcgccta
     3361 caagctgcag gcgcagaaga ggctgacgca ggagatccag agccgagagc gggacagggc
     3421 cacagcatcc cacctctccc aggccctgca ggcagcgctc caggactatg agctccaggc
     3481 agacacctac cgctgctctt tggagcccac cctggcagtg tcagccccca agagaccccg
     3541 agtggctccc ctgcaagaga gcatccaagc ccaggagaag aaccttgcaa aggcctatac
     3601 tgaggttgca gcagcacagc agcagctgct ccagcagctg gagtttgcta gaaaaatgct
     3661 ggagaagaag gagctcagtg aggacatccg aaggacccat gatgcaaagc agggctccga
     3721 gagccctgcc caagcaggga gagagtcaga ggccctgaag gcccagctgg aagaggagag
     3781 gaagcgggtg gcccgggtgc agcatgagct ggaggcgcag aggagccaac tgctgcagct
     3841 gaggacccag cggcccttgg agaggctgga ggagaaggaa gtggtagagt tctaccggga
     3901 cccccagctg gagggcagcc tgtccagggt gaaggcccag gtggaggagg agggcaagcg
     3961 gcgggctggc ctgcaggcag acctggaagt ggcagcccag aaggtcgtgc agctggaaag
     4021 caagaggaag accatgcagc ctcatctgct gaccaaggag gtcacccagg tggagaggga
     4081 ccccggcctg gacagccagg cggcccagct caggatccag atccagcagc tccgcgggga
     4141 ggatgccgtc atctcggccc ggctggaagg gctgaagaag gagctactgg cccttgagaa
     4201 gagggaggtg gacgtgaagg agaaggtcgt ggtgaaagag gtagtcaagg tggagaagaa
     4261 tctggaaatg gtcaaggcag cccaggctct gaggctgcag atggaggagg atgctgcgcg
     4321 gaggaagcag gcggaggagg ctgtggccaa gctacaggct cgcatcgaag acctggagcg
     4381 ggctatcagc tcggtggagc ccaaggtcat cgtgaaggag gtgaagaagg tggagcagga
     4441 cccagggctc ctccaggagt cctccaggct gaggagcctc ctcgaggagg agaggaccaa
     4501 gaacgcgacg ctggccaggg agctgagcga cctgcacagc aagtacagcg tggtggagaa
     4561 gcagaggccc aaagtgcagc tccaggagcg cgtccacgag atcttccagg tggatccgga
     4621 gacagagcag gagatcactc ggctcaaggc caagctgcag gagatggcgg gcaagaggag
     4681 cggtgtggag aaggaggtgg agaagctgct gcccgacctg gaggtcctgc gggcccagaa
     4741 gcccacggtg gagtacaagg aggtgaccca ggaggtggtg aggcatgaga ggagccccga
     4801 ggtgctgcgt gagatcgacc gcctgaaggc tcagctcaac gagctcgtca acagccacgg
     4861 gcgctcccag gagcagctca tccgcctgca gggtgagcgc gacgagtgga ggcgcgagcg
     4921 ggccaaggtg gagaccaaga cggtgagcaa ggaggtggtg cgccacgaga aggacccggt
     4981 gctggagaaa gaagcagagc ggctccgcca ggaggtgcgg gaggcggccc agaagaggcg
     5041 ggccgcggag gacgcggtgt acgagctgca gagcaagcgc ctgctgctgg agaggaggaa
     5101 gcccgaggag aaggtggtgg tgcaggaggt ggtggtcacc cagaaggacc cgaagctgcg
     5161 cgaggagcac agccggctga gcgggagcct ggatgaggag gtgggccggc ggcgccagct
     5221 agagcttgag gtgcagcagc tgcgggccgg cgtggaggag caggagggcc tgctcagctt
     5281 ccaggaggac cgcagcaaga agctggccgt ggagagggag ctgcggcagc tgaccttgag
     5341 gatccaggag ctcgagaagc ggcctcccac ggtgcaggag aagatcatca tggaggaagt
     5401 ggtcaagctg gagaaggacc cggacctgga gaagtccacg gaagccctgc ggtgggacct
     5461 ggaccaggag aagacccagg taaccgagct gaatcgggag tgcaagaacc tgcaggtcca
     5521 gattgacgtc ctccagaaag ccaaatcgca ggagaagacc atctacaagg aagtgatccg
     5581 ggtgcagaag gaccgcgtcc tggaagatga gcgggcccgc gtgtgggaga tgctcaacag
     5641 ggagcgcacg gcccggcagg cccgggagga ggaggcacgg cgcctgcggg agcgcattga
     5701 ccgggccgag acgctgggga gaacctggtc ccgggaggag tccgagctgc agagggcccg
     5761 ggaccaggcc gaccaggagt gtgggcggct gcagcaggag ctgcgggctc tggagaggca
     5821 gaagcagcag cagacactgc agctgcagga ggagtcgaag ctgctcagcc agaagacgga
     5881 gagcgagcga cagaaggcgg cccagcgggg ccaggagctc tcgcggctgg aggcggccat
     5941 cctccgcgag aaggaccaga tctacgagaa ggagcggacg ctccgggacc tccacgccaa
     6001 ggtgagccgg gaggagctca gccaggagac ccagacgcga gagaccaacc tttccaccaa
     6061 gatctccatc ctggaacccg agacggggaa ggacatgtcc ccatacgagg cctacaagag
     6121 gggcatcatc gacaggggcc agtacttgca gctgcaggag ctcgagtgtg actgggagga
     6181 ggtcaccacc tcggggccct gtggggagga gtctgtgctc ctggaccgca agagcgggaa
     6241 gcagtactcc atcgaggccg ccctccgctg ccggcgcatc tctaaggagg agtaccatct
     6301 gtacaaggac ggccacctgc ccatctccga gtttgcgctg cttgtagctg gggagaccaa
     6361 gccaagctcc tcactctcca tcggctctat catctccaag tccccgctcg cctccccggc
     6421 cccccagagc accagtttct tctctcccag cttctctctc gggctcggtg atgacagctt
     6481 ccctatcgcc gggatctatg acacaaccac agacaacaag tgcagcatca agacggccgt
     6541 ggccaagaac atgctggacc ccatcactgg gcagaagcta ctggaggccc aggcggccac
     6601 agggggcatc gtggacctgc tcagccgtga gcgctactct gtgcacaagg cgatggagag
     6661 gggcctgatc gagaacacct ccacacagag gctgcttaac gcccagaagg ccttcaccgg
     6721 catcgaggac cccgtcacca agaagaggct ctcggtgggc gaggccgtcc agaagggctg
     6781 gatgccccgg gagagcgtgc tcccacacct gcaggtgcag cacctgaccg gggggctcat
     6841 cgaccccaag aggacaggcc gcatccccat ccagcaggcc ctcctctccg ggatgatcag
     6901 tgaagagctg gcccagctcc tgcaggacga gtccagctac gagaaggatt tgacagaccc
     6961 catctccaag gaacggctga gctacaagga ggccatgggc cgctgccgca aagaccccct
     7021 gagcggcctg ctgctcctgc cagcggcact ggaggggtac cgctgctacc gctccgcctc
     7081 ccccaccgtc ccgcgctccc ttcgcctcga ggactacaaa gaccatgacg gtgattataa
     7141 agatcatgac atcgactaca aggatgacga tgacaagtag tgagggcccg tttaaacccg
     7201 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     7261 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     7321 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     7381 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     7441 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     7501 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     7561 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     7621 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     7681 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     7741 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     7801 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     7861 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     7921 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7981 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     8041 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     8101 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8161 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     8221 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     8281 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     8341 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     8401 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     8461 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     8521 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     8581 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     8641 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     8701 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     8761 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     8821 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     8881 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     8941 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     9001 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     9061 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     9121 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     9181 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     9241 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     9301 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9361 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     9421 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     9481 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     9541 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     9601 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     9661 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     9721 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     9781 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     9841 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9901 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9961 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    10021 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    10081 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    10141 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    10201 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    10261 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
    10321 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    10381 aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    10441 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    10501 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    10561 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    10621 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    10681 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    10741 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    10801 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    10861 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    10921 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    10981 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    11041 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    11101 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    11161 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    11221 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    11281 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    11341 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    11401 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    11461 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    11521 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    11581 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
