LOCUS V027584 6208 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V027584 VERSION V027584 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 931..1728 /label="DRAM2(NM_001349881)" /note="DRAM2(NM_001349881)" /gene="DRAM2" polyA_signal 1775..1999 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2045..2473 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2487..2816 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2883..3677 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3851..3972 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4021..4037) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4045..4061 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4069..4099) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4114..4135 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4423..5008) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5179..6039) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6040..6144) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cactagtcca gtgtggtgga attcgccacc atgtggtggt ttcagcaagg cctcagtttc 961 cttccttcag cccttgtaat ttggacatct gctgctttca tattttcata cattactgca 1021 gtaacactcc accatataga cccggcttta ccttatatca gtgacactgg tacagtagct 1081 ccagaaaaat gcttatttgg ggcaatgcta aatattgcgg cagttttatg cattgctacc 1141 atttatgttc gttataagca agttcatgct ctgagtcctg aagagaacgt tatcatcaaa 1201 ttaaacaagg ctggccttgt acttggaata ctgagttgtt taggactttc tattgtggca 1261 aacttccaga aaacaaccct ttttgctgca catgtaagtg gagctgtgct tacctttggt 1321 atgggctcat tatatatgtt tgttcagacc atcctttcct accaaatgca gcccaaaatc 1381 catggcaaac aagtcttctg gatcagactg ttgttggtta tctggtgtgg agtaagtgca 1441 cttagcatgc tgacttgctc atcagttttg cacagtggca attttgggac tgatttagaa 1501 cagaaactcc attggaaccc cgaggacaaa ggttatgtgc ttcacatgat cactactgca 1561 gcagaatggt ctatgtcatt ttccttcttt ggttttttcc tgacttacat tcgtgatttt 1621 cagaaaattt ctttacgggt ggaagccaat ttacatggat taaccctcta tgacactgca 1681 ccttgcccta ttaacaatga acgaacacgg ctactttcca gagatatttg actcgagtct 1741 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct 1801 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt 1861 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg 1921 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg 1981 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat 2041 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 2101 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 2161 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 2221 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 2281 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 2341 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 2401 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 2461 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 2521 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa 2581 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 2641 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 2701 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct 2761 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 2821 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc 2881 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat 2941 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt 3001 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac 3061 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg 3121 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc 3181 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa 3241 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc 3301 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg 3361 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg 3421 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa 3481 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg 3541 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct 3601 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc 3661 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa 3721 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 3781 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 3841 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 3901 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 3961 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg 4021 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 4081 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 4141 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 4201 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 4261 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 4321 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 4381 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 4441 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 4501 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 4561 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 4621 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 4681 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 4741 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 4801 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 4861 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 4921 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca 4981 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 5041 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 5101 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 5161 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 5221 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 5281 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 5341 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 5401 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 5461 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 5521 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 5581 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 5641 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 5701 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 5761 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 5821 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 5881 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 5941 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 6001 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 6061 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 6121 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg 6181 atcgggagat ctcccgatcc cctatggt //