LOCUS V024614 11207 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V024614 VERSION V024614 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..6697 /label="DLG5(NM_004747)" /note="DLG5(NM_004747)" /gene="DLG5" CDS 6704..6769 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 6807..7031 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 7077..7505 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 7519..7848 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 7915..8709 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 8883..9004 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(9053..9069) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 9077..9093 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(9101..9131) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 9146..9167 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(9455..10040) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(10211..11071) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(11072..11176) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggagcccc agcgccggga 961 gctgctcgcc cagtgtcagc agagcctggc ccaggccatg acggaggtgg aagccgtgct 1021 cgggctgctc gaggccgcgg gagcgctcag tcccggcgag cggcggcagc tggacgagga 1081 ggcgggaggc gccaaggcgg agctgctgct caagctgctc ttggccaagg agcgggacca 1141 cttccaggac ctgcgggcgg cgctggagaa gacgcagcct cacctgctgc ccattctcta 1201 cctgaacggc gtcgtcgggc cgccgcagcc cgccgaaggc gcgggttcta cctacagcgt 1261 cctgtccacc atgccctcag actcagaaag cagcagctcc ctcagcagtg tgggcactac 1321 cgggaaggcg ccgtccccac cacccctcct cactgaccag caagtgaatg agaaggtgga 1381 gaacctctcc attcagctgc ggctgatgac ccgggagaga aacgagctcc gcaagcgcct 1441 ggcctttgct acgcatggca cggcctttga caagaggccc taccacaggc tgaatcctga 1501 ctatgagagg ctgaagatcc agtgcgtgcg agccatgtcg gacctgcaga gcctgcagaa 1561 ccagcacacc aacgccttga agaggtgtga ggaggtggcc aaggagactg acttctacca 1621 cacactccac agccggctcc tgagtgacca gactcggctg aaggatgacg tggacatgct 1681 gaggcgggag aatgggcagc tgctgcggga gcgaaacctg ctgcagcagt catgggagga 1741 catgaagcgg ctccacgagg aggaccagaa ggagatcggt gacctccgtg cccagcagca 1801 gcaggtgttg aagcacaacg ggtcatccga gattctcaac aaactgtatg acacggccat 1861 ggacaagttg gaggtggtca agaaggacta tgacgccctt cggaagaggt acagtgagaa 1921 agtcgccatc cacaatgcag acctgagccg cctggagcag ctgggggagg agaaccagcg 1981 gttgctgaag cagacagaga tgctgaccca gcagagggac acggccatcc agctgcagca 2041 ccagtgcgcc ctctccctga ggaggtttga ggcgatccac catgagctga acaaggccac 2101 ggcgcagaac aaggacctgc agtgggagat ggagctgctg cagtcagagc tgaccgagct 2161 gagaaccacg caggtgaaga cagcaaagga gtcggagaaa tacagggagg agcgggacgc 2221 tgtgtacagc gagtacaagc tcatcatgag tgagcgtgac caggtcatct ctgagctgga 2281 caagctgcag accgaagtgg agctggccga gtccaagctc aagagcagca catctgagaa 2341 gaaggcggcc aatgaggaga tggaggcgct gcggcagatc aaagacacgg tgacaatgga 2401 tgctgggaga gccaacaagg aggttgaaat ccttcgaaag cagtgcaagg ctctgtgcca 2461 ggagctgaag gaagccctcc aggaggcgga tgtggccaag tgccggcggg actgggcctt 2521 ccaggagcga gacaagattg tagcagagcg tgacagcatc cggacactgt gtgacaacct 2581 gaggcgggag cgggaccgtg cggtgagcga gctggctgag gccctgcgca gcctggatga 2641 cacccgcaag cagaagaatg atgtcagccg cgagctgaag gagctcaagg aacagatgga 2701 atcccagttg gaaaaggagg cccggttccg acagctgatg gcccacagct cccacgactc 2761 ggccattgac acggattcca tggagtggga aacggaagtt gtagagttcg agagggagac 2821 ggaggatatt gacttgaagg cactggggtt tgatatggca gaaggtgtga atgagccttg 2881 tttcccgggg gactgtggca tatttgtcac taaagtggac aaaggaagca ttgctgatgg 2941 ccgcttaagg gtcaatgact ggctgctgag aatcaacgat gtggacctca tcaacaagga 3001 caagaagcag gccatcaagg cgctcctcaa tggggagggg gccatcaaca tggtcgtgcg 3061 gcggaggaag tccctgggtg ggaaggtggt cacgccgctg cacatcaacc tcagtggaca 3121 gaaagacagt ggcatcagtc tggagaatgg agtgtatgct gccgctgtgc tgcctggaag 3181 ccctgccgct aaagaagggt cccttgctgt gggagacagg atcgttgcga tcaatggcat 3241 tgcactggac aacaagtctc tgaatgaatg tgaatctctg ctgcgcagct gccaggactc 3301 cctgaccctg tccctcctga aggtattccc tcagagctcc tcgtggagtg gccagaacat 3361 ttttgaaaat atcaaagact ctgataagat gctgagtttt cgagcccatg gcccggaggt 3421 ccaggctcat aacaaacgga acttgataca gcacaataac tccacgcaga cagacatctt 3481 ctacacggac aggctggaag acaggaagga gccaggcccc ccaggaggca gcagctcctt 3541 tctgcataag ccattccctg ggggaccctt gcaggtctgc ccccaggcct gtcccagtgc 3601 ctctgagcgt agcctgagct ccttccgctc agatgcctct ggggaccgtg gctttgggct 3661 ggtggacgtg cgtggccggc ggccactgct gccctttgag accgaggtgg gcccctgtgg 3721 ggttggggag gcctccctgg acaaggcaga ctctgaaggc tccaacagcg gcgggacctg 3781 gcccaaggcc atgctcagct ccacggcagt gcctgagaag ctctctgttt ataaaaagcc 3841 aaagcaaaga aagtccatct ttgaccctaa cactttcaaa cgcccccaga caccccccaa 3901 aatagactac ctgcttccag gtcctgggcc tgctcactct ccccagccct ccaagagggc 3961 ggggcctctg acacccccaa aacctcccag aaggagcgac tccattaagt tccagcacag 4021 gctggagact agctccgagt cagaagccac tctggtgggc agctccccat ccactagtcc 4081 cccgagcgcc ctgccccctg acgtggaccc cggggagccc atgcacgcat caccccctcg 4141 caaggccagg gtccgcattg cttccagcta ctaccctgaa ggagatgggg actcctccca 4201 cctgccggcc aagaaatcct gtgatgagga cctcacctcc cagaaggtgg atgagctggg 4261 gcagaagcgt cgccggccaa aatctgctcc cagttttcgg ccgaagcttg ctccagtagt 4321 gattcctgct cagttcctgg aggaacagaa gtgtgtcccg gccagtggag aactctcccc 4381 ggagctccag gagtgggcac cttactcgcc tgggcattcc agccggcaca gcaacccccc 4441 gctataccct agcaggccgt ctgtgggcac tgttccccgg agtttgaccc ccagcaccac 4501 tgtgagctcc atcctgcgga accccatcta cactgtgcgc agtcacaggg tcggcccctg 4561 cagctctcca cctgcggccc gagatgctgg cccccagggt ttgcatccca gtgtccagca 4621 ccagggacgc ctgagcctgg acctgagcca caggacctgc agcgactact ccgagatgag 4681 agccacccat gggtccaact cactgccctc cagcgcccgc ctgggttctt cgagtaactt 4741 gcagttcaag gcggaacgca ttaaaatccc atcaacacca agatatccgc ggagtgtcgt 4801 gggctccgag agaggttcag tgtcacattc tgaatgcagc actcctccac agtcacccct 4861 gaacatcgac accctgtcct cttgtagcca gtcccagacc tcagcctcca cattgcccag 4921 aatcgctgtc aaccccgcgt ccctcgggga gcggagaaag gacaggcctt atgtggagga 4981 gccacgccac gtgaaggtgc agaagggctc agagccgctg ggcatctcca tcgtgagtgg 5041 agagaagggc ggcatctacg tctccaaggt gaccgtgggg agcatcgctc accaggctgg 5101 cctcgagtat ggggatcagt tactggagtt caacggcata aacctgcgga gcgccacgga 5161 gcagcaggcg cggctcatca tcgggcagca gtgtgatacc atcaccatcc tggcccagta 5221 caacccccac gtgcaccagc tcagcagcca ctcccggtcc agctcacacc tggaccctgc 5281 cggtacccac tccactctcc agggcagtgg caccaccacc ccggagcatc catctgtcat 5341 cgacccactg atggagcagg acgaggggcc tagcaccccc ccagccaagc agagcagctc 5401 caggattgcg ggagatgcca acaagaagac cctggagcca cgcgttgtct tcatcaaaaa 5461 gtcccagctg gagcttgggg tgcacttgtg tggtgggaac ctgcatgggg tgtttgtggc 5521 cgaggtggag gatgacagtc ctgccaaggg tcctgacggc ctcgtgccag gggacctcat 5581 cctggagtat ggcagcctgg acgtgcggaa caagacagtg gaggaagtct atgtggagat 5641 gctgaagccc agggatggcg tccgcctgaa ggtgcagtac cgccctgagg agttcacgaa 5701 ggccaagggc ctgcctggtg acagcttcta catcagggcc ctgtacgacc ggctggcaga 5761 tgtggagcaa gagttgagct ttaagaagga cgacatcctc tacgtggatg acaccttacc 5821 ccagggcacg ttcgggtcct ggatggcttg gcagctggac gagaatgccc agaagatcca 5881 gcgcgggcag attcccagca aatatgtgat ggaccaagaa ttctccagga ggctcagcat 5941 gtctgaagtc aaagatgaca atagcgccac aaagacgctg tcagcggctg cacgccggtc 6001 cttttttcgg aggaaacaca agcacaaacg cagcgggtcc aaggacggga aagacctgct 6061 cgccttggat gccttttcca gtgactccat tccactcttt gaagattcgg tgagcctggc 6121 ctatcagcgg gtccagaagg tggactgcac cgctctgagg cctgtcctga ttctggggcc 6181 tttgctggac gtggtgaagg agatgctggt gaatgaggct cctggcaagt tctgcagatg 6241 tccccttgag gtgatgaagg cctcccagca ggccattgag cggggtgtca aagattgcct 6301 gtttgtcgac tataagcgga gaagcggcca tttcgatgtg accactgtgg cgtcaataaa 6361 ggagatcaca gaaaagaacc gacactgcct cctggacatt gctccgcacg ctattgagcg 6421 gctccaccac atgcacatct accccattgt catcttcatc cactacaaga gcgccaagca 6481 catcaaggag cagagagacc ccatctacct gagggacaag gtgactcaga ggcattccaa 6541 agagcagttt gaggcggcgc agaagcttga gcaggagtac agcaggtact tcacaggggt 6601 catccaggga ggagccctgt caagcatttg cactcagatc ttggcaatgg tcaatcaaga 6661 acaaaataaa gtcctgtgga ttccagcctg cccgctcctc gaggactaca aagaccatga 6721 cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt agtgagggcc 6781 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 6841 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 6901 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 6961 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 7021 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc 7081 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 7141 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 7201 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 7261 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 7321 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 7381 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 7441 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 7501 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 7561 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 7621 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 7681 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 7741 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta 7801 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga 7861 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt 7921 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat 7981 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag 8041 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac 8101 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac 8161 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc 8221 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg 8281 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag 8341 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat 8401 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag 8461 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc 8521 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg 8581 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg 8641 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag 8701 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat 8761 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc 8821 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc 8881 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 8941 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 9001 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 9061 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 9121 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 9181 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 9241 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 9301 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 9361 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 9421 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 9481 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 9541 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 9601 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 9661 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 9721 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 9781 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 9841 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 9901 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 9961 gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc 10021 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 10081 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 10141 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 10201 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 10261 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 10321 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 10381 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 10441 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 10501 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 10561 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 10621 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 10681 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 10741 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 10801 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 10861 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 10921 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 10981 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 11041 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 11101 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 11161 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc //