LOCUS       V024614                11207 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024614
VERSION     V024614
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             941..6697
                     /label="DLG5(NM_004747)"
                     /note="DLG5(NM_004747)"
                     /gene="DLG5"
     CDS             6704..6769
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    6807..7031
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      7077..7505
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7519..7848
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7915..8709
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8883..9004
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9053..9069)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9077..9093
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9101..9131)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9146..9167
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9455..10040)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10211..11071)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11072..11176)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggagcccc agcgccggga
      961 gctgctcgcc cagtgtcagc agagcctggc ccaggccatg acggaggtgg aagccgtgct
     1021 cgggctgctc gaggccgcgg gagcgctcag tcccggcgag cggcggcagc tggacgagga
     1081 ggcgggaggc gccaaggcgg agctgctgct caagctgctc ttggccaagg agcgggacca
     1141 cttccaggac ctgcgggcgg cgctggagaa gacgcagcct cacctgctgc ccattctcta
     1201 cctgaacggc gtcgtcgggc cgccgcagcc cgccgaaggc gcgggttcta cctacagcgt
     1261 cctgtccacc atgccctcag actcagaaag cagcagctcc ctcagcagtg tgggcactac
     1321 cgggaaggcg ccgtccccac cacccctcct cactgaccag caagtgaatg agaaggtgga
     1381 gaacctctcc attcagctgc ggctgatgac ccgggagaga aacgagctcc gcaagcgcct
     1441 ggcctttgct acgcatggca cggcctttga caagaggccc taccacaggc tgaatcctga
     1501 ctatgagagg ctgaagatcc agtgcgtgcg agccatgtcg gacctgcaga gcctgcagaa
     1561 ccagcacacc aacgccttga agaggtgtga ggaggtggcc aaggagactg acttctacca
     1621 cacactccac agccggctcc tgagtgacca gactcggctg aaggatgacg tggacatgct
     1681 gaggcgggag aatgggcagc tgctgcggga gcgaaacctg ctgcagcagt catgggagga
     1741 catgaagcgg ctccacgagg aggaccagaa ggagatcggt gacctccgtg cccagcagca
     1801 gcaggtgttg aagcacaacg ggtcatccga gattctcaac aaactgtatg acacggccat
     1861 ggacaagttg gaggtggtca agaaggacta tgacgccctt cggaagaggt acagtgagaa
     1921 agtcgccatc cacaatgcag acctgagccg cctggagcag ctgggggagg agaaccagcg
     1981 gttgctgaag cagacagaga tgctgaccca gcagagggac acggccatcc agctgcagca
     2041 ccagtgcgcc ctctccctga ggaggtttga ggcgatccac catgagctga acaaggccac
     2101 ggcgcagaac aaggacctgc agtgggagat ggagctgctg cagtcagagc tgaccgagct
     2161 gagaaccacg caggtgaaga cagcaaagga gtcggagaaa tacagggagg agcgggacgc
     2221 tgtgtacagc gagtacaagc tcatcatgag tgagcgtgac caggtcatct ctgagctgga
     2281 caagctgcag accgaagtgg agctggccga gtccaagctc aagagcagca catctgagaa
     2341 gaaggcggcc aatgaggaga tggaggcgct gcggcagatc aaagacacgg tgacaatgga
     2401 tgctgggaga gccaacaagg aggttgaaat ccttcgaaag cagtgcaagg ctctgtgcca
     2461 ggagctgaag gaagccctcc aggaggcgga tgtggccaag tgccggcggg actgggcctt
     2521 ccaggagcga gacaagattg tagcagagcg tgacagcatc cggacactgt gtgacaacct
     2581 gaggcgggag cgggaccgtg cggtgagcga gctggctgag gccctgcgca gcctggatga
     2641 cacccgcaag cagaagaatg atgtcagccg cgagctgaag gagctcaagg aacagatgga
     2701 atcccagttg gaaaaggagg cccggttccg acagctgatg gcccacagct cccacgactc
     2761 ggccattgac acggattcca tggagtggga aacggaagtt gtagagttcg agagggagac
     2821 ggaggatatt gacttgaagg cactggggtt tgatatggca gaaggtgtga atgagccttg
     2881 tttcccgggg gactgtggca tatttgtcac taaagtggac aaaggaagca ttgctgatgg
     2941 ccgcttaagg gtcaatgact ggctgctgag aatcaacgat gtggacctca tcaacaagga
     3001 caagaagcag gccatcaagg cgctcctcaa tggggagggg gccatcaaca tggtcgtgcg
     3061 gcggaggaag tccctgggtg ggaaggtggt cacgccgctg cacatcaacc tcagtggaca
     3121 gaaagacagt ggcatcagtc tggagaatgg agtgtatgct gccgctgtgc tgcctggaag
     3181 ccctgccgct aaagaagggt cccttgctgt gggagacagg atcgttgcga tcaatggcat
     3241 tgcactggac aacaagtctc tgaatgaatg tgaatctctg ctgcgcagct gccaggactc
     3301 cctgaccctg tccctcctga aggtattccc tcagagctcc tcgtggagtg gccagaacat
     3361 ttttgaaaat atcaaagact ctgataagat gctgagtttt cgagcccatg gcccggaggt
     3421 ccaggctcat aacaaacgga acttgataca gcacaataac tccacgcaga cagacatctt
     3481 ctacacggac aggctggaag acaggaagga gccaggcccc ccaggaggca gcagctcctt
     3541 tctgcataag ccattccctg ggggaccctt gcaggtctgc ccccaggcct gtcccagtgc
     3601 ctctgagcgt agcctgagct ccttccgctc agatgcctct ggggaccgtg gctttgggct
     3661 ggtggacgtg cgtggccggc ggccactgct gccctttgag accgaggtgg gcccctgtgg
     3721 ggttggggag gcctccctgg acaaggcaga ctctgaaggc tccaacagcg gcgggacctg
     3781 gcccaaggcc atgctcagct ccacggcagt gcctgagaag ctctctgttt ataaaaagcc
     3841 aaagcaaaga aagtccatct ttgaccctaa cactttcaaa cgcccccaga caccccccaa
     3901 aatagactac ctgcttccag gtcctgggcc tgctcactct ccccagccct ccaagagggc
     3961 ggggcctctg acacccccaa aacctcccag aaggagcgac tccattaagt tccagcacag
     4021 gctggagact agctccgagt cagaagccac tctggtgggc agctccccat ccactagtcc
     4081 cccgagcgcc ctgccccctg acgtggaccc cggggagccc atgcacgcat caccccctcg
     4141 caaggccagg gtccgcattg cttccagcta ctaccctgaa ggagatgggg actcctccca
     4201 cctgccggcc aagaaatcct gtgatgagga cctcacctcc cagaaggtgg atgagctggg
     4261 gcagaagcgt cgccggccaa aatctgctcc cagttttcgg ccgaagcttg ctccagtagt
     4321 gattcctgct cagttcctgg aggaacagaa gtgtgtcccg gccagtggag aactctcccc
     4381 ggagctccag gagtgggcac cttactcgcc tgggcattcc agccggcaca gcaacccccc
     4441 gctataccct agcaggccgt ctgtgggcac tgttccccgg agtttgaccc ccagcaccac
     4501 tgtgagctcc atcctgcgga accccatcta cactgtgcgc agtcacaggg tcggcccctg
     4561 cagctctcca cctgcggccc gagatgctgg cccccagggt ttgcatccca gtgtccagca
     4621 ccagggacgc ctgagcctgg acctgagcca caggacctgc agcgactact ccgagatgag
     4681 agccacccat gggtccaact cactgccctc cagcgcccgc ctgggttctt cgagtaactt
     4741 gcagttcaag gcggaacgca ttaaaatccc atcaacacca agatatccgc ggagtgtcgt
     4801 gggctccgag agaggttcag tgtcacattc tgaatgcagc actcctccac agtcacccct
     4861 gaacatcgac accctgtcct cttgtagcca gtcccagacc tcagcctcca cattgcccag
     4921 aatcgctgtc aaccccgcgt ccctcgggga gcggagaaag gacaggcctt atgtggagga
     4981 gccacgccac gtgaaggtgc agaagggctc agagccgctg ggcatctcca tcgtgagtgg
     5041 agagaagggc ggcatctacg tctccaaggt gaccgtgggg agcatcgctc accaggctgg
     5101 cctcgagtat ggggatcagt tactggagtt caacggcata aacctgcgga gcgccacgga
     5161 gcagcaggcg cggctcatca tcgggcagca gtgtgatacc atcaccatcc tggcccagta
     5221 caacccccac gtgcaccagc tcagcagcca ctcccggtcc agctcacacc tggaccctgc
     5281 cggtacccac tccactctcc agggcagtgg caccaccacc ccggagcatc catctgtcat
     5341 cgacccactg atggagcagg acgaggggcc tagcaccccc ccagccaagc agagcagctc
     5401 caggattgcg ggagatgcca acaagaagac cctggagcca cgcgttgtct tcatcaaaaa
     5461 gtcccagctg gagcttgggg tgcacttgtg tggtgggaac ctgcatgggg tgtttgtggc
     5521 cgaggtggag gatgacagtc ctgccaaggg tcctgacggc ctcgtgccag gggacctcat
     5581 cctggagtat ggcagcctgg acgtgcggaa caagacagtg gaggaagtct atgtggagat
     5641 gctgaagccc agggatggcg tccgcctgaa ggtgcagtac cgccctgagg agttcacgaa
     5701 ggccaagggc ctgcctggtg acagcttcta catcagggcc ctgtacgacc ggctggcaga
     5761 tgtggagcaa gagttgagct ttaagaagga cgacatcctc tacgtggatg acaccttacc
     5821 ccagggcacg ttcgggtcct ggatggcttg gcagctggac gagaatgccc agaagatcca
     5881 gcgcgggcag attcccagca aatatgtgat ggaccaagaa ttctccagga ggctcagcat
     5941 gtctgaagtc aaagatgaca atagcgccac aaagacgctg tcagcggctg cacgccggtc
     6001 cttttttcgg aggaaacaca agcacaaacg cagcgggtcc aaggacggga aagacctgct
     6061 cgccttggat gccttttcca gtgactccat tccactcttt gaagattcgg tgagcctggc
     6121 ctatcagcgg gtccagaagg tggactgcac cgctctgagg cctgtcctga ttctggggcc
     6181 tttgctggac gtggtgaagg agatgctggt gaatgaggct cctggcaagt tctgcagatg
     6241 tccccttgag gtgatgaagg cctcccagca ggccattgag cggggtgtca aagattgcct
     6301 gtttgtcgac tataagcgga gaagcggcca tttcgatgtg accactgtgg cgtcaataaa
     6361 ggagatcaca gaaaagaacc gacactgcct cctggacatt gctccgcacg ctattgagcg
     6421 gctccaccac atgcacatct accccattgt catcttcatc cactacaaga gcgccaagca
     6481 catcaaggag cagagagacc ccatctacct gagggacaag gtgactcaga ggcattccaa
     6541 agagcagttt gaggcggcgc agaagcttga gcaggagtac agcaggtact tcacaggggt
     6601 catccaggga ggagccctgt caagcatttg cactcagatc ttggcaatgg tcaatcaaga
     6661 acaaaataaa gtcctgtgga ttccagcctg cccgctcctc gaggactaca aagaccatga
     6721 cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt agtgagggcc
     6781 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     6841 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     6901 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     6961 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     7021 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     7081 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7141 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7201 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     7261 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     7321 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     7381 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     7441 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     7501 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     7561 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     7621 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     7681 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7741 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     7801 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     7861 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     7921 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     7981 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     8041 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     8101 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     8161 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     8221 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     8281 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     8341 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     8401 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     8461 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     8521 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     8581 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     8641 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     8701 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     8761 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     8821 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     8881 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8941 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     9001 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     9061 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     9121 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     9181 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     9241 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     9301 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9361 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9421 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9481 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9541 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9601 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9661 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9721 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9781 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9841 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     9901 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9961 gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc
    10021 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    10081 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    10141 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    10201 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    10261 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    10321 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    10381 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    10441 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10501 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10561 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10621 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10681 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10741 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10801 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10861 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10921 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10981 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    11041 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    11101 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    11161 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
