LOCUS V028798 8584 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V028798 VERSION V028798 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 874..1590 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="EGFP" CDS 1642..4074 /label="DEPDC1(NM_001114120)" /note="DEPDC1(NM_001114120)" /gene="DEPDC1" polyA_signal 4151..4375 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 4421..4849 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4863..5192 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5259..6053 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6227..6348 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6397..6413) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6421..6437 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6445..6475) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6490..6511 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6799..7384) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7555..8415) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8416..8520) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgcc accatggtga gcaagggcga ggagctgttc 901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 1561 atcactctcg gcatggacga gctgtacaag ggaggcggag gtagtggagg agggggaagc 1621 ggaggcggag ggagtgaatt catggagagt cagggtgtgc ctcccgggcc ttatcgggcc 1681 accaagctgt ggaatgaagt taccacatct tttcgagcag gaatgcctct aagaaaacac 1741 agacaacact ttaaaaaata tggcaattgt ttcacagcag gagaagcagt ggattggctt 1801 tatgacctat taagaaataa tagcaatttt ggtcctgaag ttacaaggca acagactatc 1861 caactgttga ggaaatttct taagaatcat gtaattgaag atatcaaagg gaggtgggga 1921 tcagaaaatg ttgatgataa caaccagctc ttcagatttc ctgcaacttc gccacttaaa 1981 actctaccac gaaggtatcc agaattgaga aaaaacaaca tagagaactt ttccaaagat 2041 aaagatagca tttttaaatt acgaaactta tctcgtagaa ctcctaaaag gcatggatta 2101 catttatctc aggaaaatgg cgagaaaata aagcatgaaa taatcaatga agatcaagaa 2161 aatgcaattg ataatagaga actaagccag gaagatgttg aagaagtttg gagatatgtt 2221 attctgatct acctgcaaac cattttaggt gtgccatccc tagaagaagt cataaatcca 2281 aaacaagtaa ttccccaata tataatgtac aacatggcca atacaagtaa acgtggagta 2341 gttatactac aaaacaaatc agatgacctc cctcactggg tattatctgc catgaagtgc 2401 ctagcaaatt ggccaagaag caatgatatg aataatccaa cttatgttgg atttgaacga 2461 gatgtattca gaacaatcgc agattatttt ctagatctcc ctgaacctct acttactttt 2521 gaatattacg aattatttgt aaacattttg gttgtttgtg gctacatcac agtttcagat 2581 agatccagtg ggatacataa aattcaagat gatccacagt cttcaaaatt ccttcactta 2641 aacaatttga attccttcaa atcaactgag tgccttcttc tcagtctgct tcatagagaa 2701 aaaaacaaag aagaatcaga ttctactgag agactacaga taagcaatcc aggatttcaa 2761 gaaagatgtg ctaagaaaat gcagctagtt aatttaagaa acagaagagt gagtgctaat 2821 gacataatgg gaggaagttg tcataattta atagggttaa gtaatatgca tgatctatcc 2881 tctaacagca aaccaaggtg ctgttctttg gaaggaattg tagatgtgcc agggaattca 2941 agtaaagagg catccagtgt ctttcatcaa tcttttccga acatagaagg acaaaataat 3001 aaactgtttt tagagtctaa gcccaaacag gaattcctgt tgaatcttca ttcagaggaa 3061 aatattcaaa agccattcag tgctggtttt aagagaacct ctactttgac tgttcaagac 3121 caagaggagt tgtgtaatgg gaaatgcaag tcaaaacagc tttgtaggtc tcagagtttg 3181 cttttaagaa gtagtacaag aaggaatagt tatatcaata caccagtggc tgaaattatc 3241 atgaaaccaa atgttggaca aggcagcaca agtgtgcaaa cagctatgga aagtgaactc 3301 ggagagtcta gtgccacaat caataaaaga ctctgcaaaa gtacaataga actttcagaa 3361 aattctttac ttccagcttc ttctatgttg actggcacac aaagcttgct gcaacctcat 3421 ttagagaggg ttgccatcga tgctctacag ttatgttgtt tgttacttcc cccaccaaat 3481 cgtagaaagc ttcaactttt aatgcgtatg atttcccgaa tgagtcaaaa tgttgatatg 3541 cccaaacttc atgatgcaat gggtacgagg tcactgatga tacatacctt ttctcgatgt 3601 gtgttatgct gtgctgaaga agtggatctt gatgagcttc ttgctggaag attagtttct 3661 ttcttaatgg atcatcatca ggaaattctt caagtaccct cttacttaca gactgcagtg 3721 gaaaaacatc ttgactactt aaaaaaggga catattgaaa atcctggaga tggactattt 3781 gctcctttgc caacttactc atactgtaag cagattagtg ctcaggagtt tgatgagcaa 3841 aaagtttcta cctctcaagc tgcaattgca gaacttttag aaaatattat taaaaacagg 3901 agtttacctc taaaggagaa aagaaaaaaa ctaaaacagt ttcagaagga atatcctttg 3961 atatatcaga aaagatttcc aaccacggag agtgaagcag cactttttgg tgacaaacct 4021 acaatcaagc aaccaatgct gattttaaga aaaccaaagt tccgtagtct aagataagga 4081 tcctgatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga 4141 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct 4201 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca 4261 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag 4321 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct 4381 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca 4441 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 4501 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 4561 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 4621 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 4681 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 4741 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 4801 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga 4861 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 4921 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 4981 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 5041 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 5101 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 5161 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 5221 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 5281 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 5341 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 5401 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 5461 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 5521 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 5581 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 5641 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 5701 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 5761 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 5821 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 5881 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 5941 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 6001 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 6061 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 6121 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 6181 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 6241 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 6301 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 6361 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 6421 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 6481 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 6541 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 6601 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 6661 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 6721 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 6781 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 6841 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 6901 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 6961 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 7021 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 7081 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 7141 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 7201 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 7261 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 7321 caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 7381 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 7441 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 7501 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 7561 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 7621 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 7681 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 7741 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 7801 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 7861 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 7921 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 7981 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 8041 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 8101 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 8161 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 8221 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 8281 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 8341 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 8401 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 8461 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 8521 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta 8581 tggt //