LOCUS       V042738                 8445 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042738
VERSION     V042738
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             874..1590
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1642..3936
                     /label="DDX27(NM_017895)"
                     /note="DDX27(NM_017895)"
                     /gene="DDX27"
     polyA_signal    4012..4236
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4282..4710
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4724..5053
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5120..5914
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6088..6209
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6258..6274)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6282..6298
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6306..6336)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6351..6372
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6660..7245)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7416..8276)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8277..8381)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgcc accatggtga gcaagggcga ggagctgttc
      901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1561 atcactctcg gcatggacga gctgtacaag ggaggcggag gtagtggagg agggggaagc
     1621 ggaggcggag ggagtgaatt catgcttgcg gacctcggct taatcggaac cataggcgag
     1681 gatgacgagg tgccggtgga gcccgagtct gactccgggg acgaggaaga ggaggggccc
     1741 attgtgctgg gcagacgaca aaaagctttg gggaagaacc gcagtgctga tttcaaccct
     1801 gatttcgttt tcactgagaa ggaggggacg tacgatggca gctgggccct ggctgatgtc
     1861 atgagccaac tcaagaagaa gagggcagcc actacattag atgagaagat tgagaaagtt
     1921 cgaaagaaaa ggaaaacaga ggataaagaa gccaagtctg ggaagttgga aaaggagaaa
     1981 gaagcaaagg aaggctctga accaaaggag caggaagacc ttcaagagaa tgatgaggaa
     2041 ggctcagaag atgaagcctc ggagactgac tactcatcag ctgatgagaa catcctcacc
     2101 aaagcagata cactcaaagt aaaggatcgg aagaagaaga agaagaaagg acaggaagca
     2161 ggaggatttt ttgaagatgc atctcagtac gatgaaaacc tctcgttcca ggacatgaac
     2221 ctttcccgcc ctcttctgaa ggccattaca gccatgggct tcaagcagcc caccccgatc
     2281 cagaaggcgt gcatacctgt gggtctattg gggaaggaca tctgtgcctg tgcagccact
     2341 gggacaggta aaactgccgc ctttgccctg cctgttttgg agcgtctgat ttataaaccc
     2401 cgccaggctc cagtcacccg cgtgctggtg ctagtgccca cccgagagct gggcatccag
     2461 gtgcactctg tcaccagaca gctggcccag ttctgcaaca tcaccacctg cctggctgtg
     2521 ggcggcttgg atgtgaagtc tcaggaagca gctcttcggg cagcgcctga catcctcatc
     2581 gccaccccag gccggctcat cgatcacctc cacaactgcc cttccttcca cctgagcagc
     2641 atcgaggtgc tcatcctgga cgaggctgac aggatgctgg atgagtactt tgaggagcag
     2701 atgaaggaga tcatccgaat gtgttcccac caccgccaga ccatgctctt ctcggccacc
     2761 atgacagacg aggtgaaaga tctggcttct gtctccttga agaatcctgt ccggatattt
     2821 gtgaacagca acacagatgt ggctcccttc ctgcggcagg agttcatccg gatccggcct
     2881 aatcgtgaag gagaccggga agccatcgtg gcagctttgt tgacgaggac cttcactgac
     2941 catgtgatgc tgttcacgca aaccaagaag caggcccacc gcatgcacat cctcctgggg
     3001 ctcatggggc tgcaggtggg tgagctccat ggcaacttgt cacagacgca gcggctggag
     3061 gccctccggc gttttaagga tgaacagatt gacatcctcg tggccactga tgtggcagcc
     3121 cgtggacttg acattgaggg ggtcaaaacg gtaatcaact tcacaatgcc taataccatc
     3181 aaacattatg tccaccgggt ggggcgaaca gcacgtgctg gcagggctgg gcgctcagtc
     3241 tctctggtgg gagaagatga gcggaagatg ctgaaggaga ttgtaaaagc tgccaaggcc
     3301 cctgtgaagg ccaggatact tccccaagat gtcatcctca aattccggga caagattgag
     3361 aaaatggaga aagatgtgta tgcagttctg cagctagagg cggaggaaaa agagatgcag
     3421 cagtcagaag cccagatcaa tacagcaaag cggctcctgg agaaggggaa ggaggcagtg
     3481 gtccaagagc ccgagaggag ctggttccag accaaagaag agaggaagaa ggagaaaatt
     3541 gccaaagctc tgcaggaatt tgacttggcc ttaagaggaa agaagaaaag gaagaagttt
     3601 atgaaggatg ccaaaaaaaa gggggagatg acagcagagg aaaggtctca gtttgaaatc
     3661 ctcaaggcgc agatgtttgc tgaacggcta gcgaagagga atcgcagagc caagcgggcc
     3721 cgagcaatgc ccgaggagga gccagtgaga ggtcctgcca agaagcaaaa gcaggggaag
     3781 aaatctgtat ttgatgaaga actcaccaac acaagcaaga aggccctgaa acagtatcga
     3841 gctggccctt cctttgaaga aaggaaacag ttgggcttgc cccaccagag acgaggagga
     3901 aactttaaat ctaaatccag atacaagagg aggaagtagg atcctgatat ccagcacagt
     3961 ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     4021 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     4081 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     4141 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     4201 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     4261 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4321 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4381 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4441 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4501 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4561 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4621 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4681 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     4741 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4801 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4861 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4921 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4981 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5041 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     5101 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     5161 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     5221 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     5281 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     5341 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     5401 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     5461 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     5521 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     5581 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     5641 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     5701 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     5761 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     5821 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     5881 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     5941 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     6001 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     6061 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     6121 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6181 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     6241 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6301 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6361 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6421 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6481 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6541 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6601 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6661 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6721 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6781 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6841 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6901 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6961 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7021 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7081 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7141 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt
     7201 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7261 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7321 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7381 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7441 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7501 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7561 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7621 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7681 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7741 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7801 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7861 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7921 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7981 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8041 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8101 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8161 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8221 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8281 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8341 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8401 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggt
//
