LOCUS V026638 6545 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V026638 VERSION V026638 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..2035 /label="Cxadr(NM_001025192)" /note="Cxadr(NM_001025192)" /gene="Cxadr" CDS 2042..2107 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 2145..2369 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2415..2843 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2857..3186 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3253..4047 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4221..4342 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4391..4407) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4415..4431 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4439..4469) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4484..4505 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4793..5378) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5549..6409) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6410..6514) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggcgcgcc tactgtgctt 961 cgtgctcttg tgcgggatcg cggatttcac cagtggtttg agcatcacta cacccgaaca 1021 gaggatcgaa aaagccaaag gggaaactgc gtatctacca tgcaagttta ctctcagtcc 1081 cgaagaccag ggaccactgg acattgaatg gctgatatcc ccgtctgata accagatagt 1141 ggatcaagtg atcattttgt attctggaga caaaatttat gataactact atccggatct 1201 gaaaggacgg gtacatttta cgagtaacga tgtcaagtct ggcgacgcat ctataaatgt 1261 gaccaacctg cagctgtcgg acattggcac ttaccagtgc aaagtgaaga aagcccctgg 1321 ggttgcaaat aagaaattcc tgctgaccgt tcttgttaag ccttcaggta caagatgctt 1381 cgtggatgga tcggaagaga ttggaaatga cttcaagcta aaatgtgaac ccaaggaagg 1441 ctcccttcca ctacagtttg aatggcagaa actgtcggac tcccagacaa tgcctacgcc 1501 atggctggca gaaatgacgt caccagttat atctgtgaag aacgccagtt ctgagtattc 1561 tgggacatac agctgcacgg ttcaaaacag agtgggctct gaccagtgta tgctgcgact 1621 agacgttgtc ccaccctcca accgagccgg aacgatcgcg ggcgccgtca tagggacgct 1681 gctggccctt gtgctcatcg gggccatcct cttctgctgt cacaggaaac gcagagaaga 1741 gaagtacgag aaggaagttc atcatgatat cagggaagat gtgcctcctc caaagagtcg 1801 gacatccact gccaggagct atattggcag caaccattcc tccctgggat ccatgtcccc 1861 ctctaacatg gaggggtatt ccaagacgca gtataaccaa gtccccagtg aagactttga 1921 acgtgcgcct cagagcccga ctctggcacc cgctaaggta gctgccccta atctcagtcg 1981 aatgggagcg gttcctgtga tgattcctgc acagagcaag gacgggtcta tagtactcga 2041 ggactacaaa gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga 2101 tgacaagtag tgagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg 2161 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 2221 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 2281 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag 2341 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc 2401 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 2461 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 2521 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 2581 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 2641 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 2701 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 2761 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 2821 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag 2881 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 2941 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 3001 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 3061 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 3121 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 3181 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga 3241 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg 3301 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg 3361 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc 3421 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct 3481 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa 3541 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg 3601 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa 3661 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat 3721 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg 3781 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc 3841 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac 3901 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg 3961 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc 4021 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag 4081 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg 4141 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 4201 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca 4261 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 4321 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg 4381 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 4441 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 4501 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 4561 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 4621 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 4681 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 4741 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 4801 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 4861 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 4921 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 4981 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 5041 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 5101 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 5161 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 5221 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 5281 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt 5341 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 5401 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 5461 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 5521 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 5581 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 5641 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 5701 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 5761 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 5821 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 5881 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 5941 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 6001 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 6061 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 6121 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 6181 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 6241 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 6301 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 6361 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 6421 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 6481 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 6541 acgtc //