LOCUS V028061 5764 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V028061 VERSION V028061 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 931..1254 /label="Cisd1(NM_001106385)" /note="Cisd1(NM_001106385)" /gene="Cisd1" CDS 1255..1284 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" polyA_signal 1331..1555 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 1601..2029 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2043..2372 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2439..3233 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3407..3528 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3577..3593) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3601..3617 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3625..3655) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 3670..3691 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(3979..4564) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(4735..5595) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5596..5700) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cactagtcca gtgtggtgga attcgccacc atgggcctca gctccgactc ccctgtgcga 961 gttgagtgga tcgccgccgt gacctttgct gctggcacag ccgctctcgg ttacctggct 1021 tacaagaagt tctacgctaa agagagtcgc accaaagcta tggtgaacct tcagatccag 1081 aaagacaacc cgaaggttgt gcacgccttc gacatggagg atctggggga taaggccgtg 1141 tattgccggt gctggaggtc taaaaagttc ccgttctgcg atggggctca cataaagcac 1201 aatgaggaga ctggagacaa cgtgggacct ctgatcatca agaaaaaaga aactgagcag 1261 aagctgatct cagaggagga cctgtagctc gagtctagag ggcccgttta aacccgctga 1321 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct 1381 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca 1441 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag 1501 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct 1561 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca 1621 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 1681 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 1741 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 1801 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 1861 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 1921 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 1981 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga 2041 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 2101 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 2161 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 2221 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 2281 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 2341 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 2401 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 2461 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 2521 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 2581 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 2641 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 2701 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 2761 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 2821 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 2881 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 2941 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 3001 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 3061 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 3121 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 3181 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 3241 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 3301 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 3361 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 3421 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 3481 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 3541 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 3601 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 3661 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 3721 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 3781 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 3841 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 3901 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 3961 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 4021 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 4081 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 4141 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 4201 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 4261 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 4321 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 4381 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 4441 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 4501 caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 4561 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 4621 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 4681 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 4741 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 4801 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 4861 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 4921 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 4981 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 5041 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 5101 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 5161 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 5221 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 5281 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 5341 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 5401 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 5461 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 5521 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 5581 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 5641 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 5701 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta 5761 tggt //