LOCUS V037626 7745 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V037626 VERSION V037626 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..3184 /label="CUL4A(NM_001008895)" /note="CUL4A(NM_001008895)" /gene="CUL4A" CDS 3191..3217 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3221..3247 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3251..3277 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" polyA_signal 3312..3536 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3582..4010 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4024..4353 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4420..5214 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5388..5509 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5558..5574) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5582..5598 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5606..5636) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5651..5672 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5960..6545) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6716..7576) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7577..7681) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg gcggacgagg ccccgcggaa gggcagcttc tcggcgctcg tgggccgcac 961 caacggcctc accaagcccg cggccctggc cgccgcgccc gccaagccgg ggggcgcggg 1021 cggctccaag aagctggtca tcaagaactt ccgagacaga cctcggctgc ccgacaacta 1081 cacgcaggac acgtggcgga agctgcacga ggcggtgcgg gccgtgcaga gcagcacctc 1141 catcaggtac aacctcgagg agctctacca ggctgtggaa aatctctgtt ctcacaaagt 1201 ctccccaatg ctctacaagc aactgcgtca ggcctgtgaa gaccacgtcc aggcacagat 1261 ccttccgttt agagaagact cactagatag tgttttattt ttaaagaaga ttaacacgtg 1321 ctggcaggac cactgcagac aaatgatcat gatcagaagc atcttcctgt tcttggaccg 1381 cacctatgtg ctgcagaact ccacgctgcc ctccatctgg gatatgggat tagaactgtt 1441 tagaacccat attattagtg ataaaatggt tcagagtaaa accattgatg gaatcctact 1501 gctgatcgag cgcgagagga gcggcgaggc cgtggaccgg agcctgttgc ggagcctcct 1561 gggcatgctg tctgacctgc aggtgtataa agattcattt gaactgaaat ttttggaaga 1621 gactaattgc ttatatgctg ccgaaggcca aaggttaatg caggaaagag aggttccaga 1681 atatcttaac catgtaagta aacgcttaga ggaagaggga gacagagtaa tcacttactt 1741 ggaccacagc acacagaaac cactgattgc ttgtgtggag aaacagctat taggagaaca 1801 tttaacagca attctgcaga aagggctcga ccacttactg gatgagaaca gagtgccgga 1861 cctcgcacag atgtaccagc tgttcagccg ggtgaggggc gggcagcagg cgctgctgca 1921 gcactggagc gagtacatca agacttttgg aacagcgatc gtaatcaatc ctgagaaaga 1981 caaagacatg gtccaagacc tgttggactt caaggacaag gtggaccacg tgatcgaggt 2041 ctgcttccag aagaatgagc ggttcgtcaa cctgatgaag gagtcctttg agacgttcat 2101 caacaagaga cccaacaagc ctgcagaact gatcgcaaag catgtggatt caaagttaag 2161 agcaggcaac aaagaagcca cagacgagga gctggagcgg acgttggaca agatcatgat 2221 cctgttcagg tttatccacg gtaaagatgt ctttgaagca ttttataaaa aagatttggc 2281 aaaaagactc cttgttggga aaagtgcctc agtcgatgct gaaaagtcta tgttgtcaaa 2341 gctcaagcat gagtgcggtg cagccttcac cagcaagctg gaaggcatgt tcaaggacat 2401 ggagctttcg aaggacatca tggttcattt caagcagcat atgcagaatc agagtgactc 2461 aggccctata gacctcacag tgaacatact cacaatgggc tactggccaa catacacgcc 2521 catggaagtg cacttaaccc cagaaatgat taaacttcag gaagtattta aggcatttta 2581 tcttggaaag cacagtggtc gaaaacttca gtggcaaact actttgggac atgctgtttt 2641 aaaagcggag tttaaagaag ggaagaagga attccaggtg tccctcttcc agacactggt 2701 gctcctcatg ttcaacgagg gagatggctt cagctttgag gagataaaaa tggccacggg 2761 gatagaggat agtgaattgc gcagaacgct gcagtccctg gcctgtggca aagcacgtgt 2821 gctgattaaa agtcccaaag gaaaggaagt ggaagatgga gacaagttca tttttaatgg 2881 agagttcaag cacaagttgt ttagaataaa gatcaatcaa attcagatga aggaaactgt 2941 tgaggaacag gttagcacca ctgagagagt gtttcaggat agacaatatc agattgatgc 3001 tgctatcgtc agaataatga agatgagaaa gactcttggt cataatcttc tagtttctga 3061 attatataat cagctgaaat ttccagtaaa gcctggagat ttgaaaaaga gaattgaatc 3121 tctgatagac agagactata tggagagaga caaagacaat ccgaatcagt accactacgt 3181 ggccctcgag tacccatacg acgtacctga ttatgcaact tatccttatg atgtacctga 3241 ttatgctagc tatccttatg atgtcccaga ctacgcatga gggcccgttt aaacccgctg 3301 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc 3361 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc 3421 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa 3481 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc 3541 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc 3601 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 3661 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 3721 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 3781 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 3841 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 3901 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 3961 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg 4021 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 4081 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 4141 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 4201 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 4261 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga 4321 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt 4381 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg 4441 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag 4501 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt 4561 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta 4621 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg 4681 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt 4741 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat 4801 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg 4861 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca 4921 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc 4981 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc 5041 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat 5101 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc 5161 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga 5221 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt 5281 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga 5341 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg 5401 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 5461 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta 5521 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 5581 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 5641 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 5701 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 5761 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 5821 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 5881 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 5941 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 6001 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 6061 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 6121 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 6181 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 6241 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 6301 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 6361 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 6421 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 6481 ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 6541 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 6601 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 6661 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 6721 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 6781 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 6841 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 6901 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 6961 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 7021 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 7081 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 7141 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 7201 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 7261 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 7321 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 7381 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 7441 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 7501 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 7561 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 7621 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 7681 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct 7741 atggt //