LOCUS       V037626                 7745 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037626
VERSION     V037626
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3184
                     /label="CUL4A(NM_001008895)"
                     /note="CUL4A(NM_001008895)"
                     /gene="CUL4A"
     CDS             3191..3217
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3221..3247
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3251..3277
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3312..3536
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3582..4010
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4024..4353
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4420..5214
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5388..5509
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5558..5574)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5582..5598
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5606..5636)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5651..5672
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5960..6545)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6716..7576)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7577..7681)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gcggacgagg ccccgcggaa gggcagcttc tcggcgctcg tgggccgcac
      961 caacggcctc accaagcccg cggccctggc cgccgcgccc gccaagccgg ggggcgcggg
     1021 cggctccaag aagctggtca tcaagaactt ccgagacaga cctcggctgc ccgacaacta
     1081 cacgcaggac acgtggcgga agctgcacga ggcggtgcgg gccgtgcaga gcagcacctc
     1141 catcaggtac aacctcgagg agctctacca ggctgtggaa aatctctgtt ctcacaaagt
     1201 ctccccaatg ctctacaagc aactgcgtca ggcctgtgaa gaccacgtcc aggcacagat
     1261 ccttccgttt agagaagact cactagatag tgttttattt ttaaagaaga ttaacacgtg
     1321 ctggcaggac cactgcagac aaatgatcat gatcagaagc atcttcctgt tcttggaccg
     1381 cacctatgtg ctgcagaact ccacgctgcc ctccatctgg gatatgggat tagaactgtt
     1441 tagaacccat attattagtg ataaaatggt tcagagtaaa accattgatg gaatcctact
     1501 gctgatcgag cgcgagagga gcggcgaggc cgtggaccgg agcctgttgc ggagcctcct
     1561 gggcatgctg tctgacctgc aggtgtataa agattcattt gaactgaaat ttttggaaga
     1621 gactaattgc ttatatgctg ccgaaggcca aaggttaatg caggaaagag aggttccaga
     1681 atatcttaac catgtaagta aacgcttaga ggaagaggga gacagagtaa tcacttactt
     1741 ggaccacagc acacagaaac cactgattgc ttgtgtggag aaacagctat taggagaaca
     1801 tttaacagca attctgcaga aagggctcga ccacttactg gatgagaaca gagtgccgga
     1861 cctcgcacag atgtaccagc tgttcagccg ggtgaggggc gggcagcagg cgctgctgca
     1921 gcactggagc gagtacatca agacttttgg aacagcgatc gtaatcaatc ctgagaaaga
     1981 caaagacatg gtccaagacc tgttggactt caaggacaag gtggaccacg tgatcgaggt
     2041 ctgcttccag aagaatgagc ggttcgtcaa cctgatgaag gagtcctttg agacgttcat
     2101 caacaagaga cccaacaagc ctgcagaact gatcgcaaag catgtggatt caaagttaag
     2161 agcaggcaac aaagaagcca cagacgagga gctggagcgg acgttggaca agatcatgat
     2221 cctgttcagg tttatccacg gtaaagatgt ctttgaagca ttttataaaa aagatttggc
     2281 aaaaagactc cttgttggga aaagtgcctc agtcgatgct gaaaagtcta tgttgtcaaa
     2341 gctcaagcat gagtgcggtg cagccttcac cagcaagctg gaaggcatgt tcaaggacat
     2401 ggagctttcg aaggacatca tggttcattt caagcagcat atgcagaatc agagtgactc
     2461 aggccctata gacctcacag tgaacatact cacaatgggc tactggccaa catacacgcc
     2521 catggaagtg cacttaaccc cagaaatgat taaacttcag gaagtattta aggcatttta
     2581 tcttggaaag cacagtggtc gaaaacttca gtggcaaact actttgggac atgctgtttt
     2641 aaaagcggag tttaaagaag ggaagaagga attccaggtg tccctcttcc agacactggt
     2701 gctcctcatg ttcaacgagg gagatggctt cagctttgag gagataaaaa tggccacggg
     2761 gatagaggat agtgaattgc gcagaacgct gcagtccctg gcctgtggca aagcacgtgt
     2821 gctgattaaa agtcccaaag gaaaggaagt ggaagatgga gacaagttca tttttaatgg
     2881 agagttcaag cacaagttgt ttagaataaa gatcaatcaa attcagatga aggaaactgt
     2941 tgaggaacag gttagcacca ctgagagagt gtttcaggat agacaatatc agattgatgc
     3001 tgctatcgtc agaataatga agatgagaaa gactcttggt cataatcttc tagtttctga
     3061 attatataat cagctgaaat ttccagtaaa gcctggagat ttgaaaaaga gaattgaatc
     3121 tctgatagac agagactata tggagagaga caaagacaat ccgaatcagt accactacgt
     3181 ggccctcgag tacccatacg acgtacctga ttatgcaact tatccttatg atgtacctga
     3241 ttatgctagc tatccttatg atgtcccaga ctacgcatga gggcccgttt aaacccgctg
     3301 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3361 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3421 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3481 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     3541 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     3601 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3661 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3721 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3781 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3841 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3901 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     3961 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     4021 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4081 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4141 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4201 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4261 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     4321 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     4381 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     4441 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     4501 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     4561 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     4621 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     4681 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     4741 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     4801 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     4861 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     4921 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     4981 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     5041 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     5101 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     5161 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     5221 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     5281 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     5341 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     5401 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5461 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     5521 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     5581 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     5641 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     5701 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     5761 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     5821 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     5881 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5941 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6001 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6061 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6121 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6181 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6241 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6301 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6361 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     6421 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6481 ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6541 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6601 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6661 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6721 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6781 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6841 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6901 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6961 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7021 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7081 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7141 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7201 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7261 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7321 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7381 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7441 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7501 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7561 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7621 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7681 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     7741 atggt
//
