LOCUS       V047675                 7706 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047675
VERSION     V047675
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3226
                     /label="CPT1A(NM_001876)"
                     /note="CPT1A(NM_001876)"
                     /gene="CPT1A"
     polyA_signal    3273..3497
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3543..3971
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3985..4314
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4381..5175
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5349..5470
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5519..5535)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5543..5559
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5567..5597)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5612..5633
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5921..6506)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6677..7537)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7538..7642)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gcagaagctc accaagctgt ggcctttcag ttcacggtca ctccggacgg
      961 gattgacctg cggctgagcc atgaagctct tagacaaatc tatctctctg gacttcattc
     1021 ctggaaaaag aagttcatca gattcaagaa cggcatcatc actggcgtgt acccggcaag
     1081 cccctccagt tggcttatcg tggtggtggg cgtgatgaca acgatgtacg ccaagatcga
     1141 cccctcgtta ggaataattg caaaaatcaa tcggactctg gaaacggcca actgcatgtc
     1201 cagccagacg aagaacgtgg tcagcggcgt gctgtttggc accggcctgt gggtggccct
     1261 catcgtcacc atgcgctact ccctgaaagt gctgctctcc taccacgggt ggatgttcac
     1321 tgagcacggc aagatgagtc gtgccaccaa gatctggatg ggtatggtca agatcttttc
     1381 aggccgaaaa cccatgttgt acagcttcca gacatcgctg cctcgcctgc cggtcccggc
     1441 tgtcaaagac actgtgaaca ggtatctaca gtcggtgagg cctcttatga aggaagaaga
     1501 cttcaaacgg atgacagcac ttgctcaaga ttttgctgtc ggtcttggac caagattaca
     1561 gtggtatttg aagttaaaat cctggtgggc tacaaattac gtgagcgact ggtgggagga
     1621 gtacatctac ctccgaggac gagggccgct catggtgaac agcaactatt atgccatgga
     1681 tctgctgtat atccttccaa ctcacattca ggcagcaaga gccggcaacg ccatccatgc
     1741 catcctgctt tacaggcgca aactggaccg ggaggaaatc aaaccaattc gtcttttggg
     1801 atccacgatt ccactctgct ccgctcagtg ggagcggatg tttaatactt cccggatccc
     1861 aggagaggag acagacacca tccagcacat gagagacagc aagcacatcg tcgtgtacca
     1921 tcgaggacgc tacttcaagg tctggctcta ccatgatggg cggctgctga agccccggga
     1981 gatggagcag cagatgcaga ggatcctgga caatacctcg gagcctcagc ccggggaggc
     2041 caggctggca gccctcaccg caggagacag agttccctgg gccaggtgtc gtcaggccta
     2101 ttttggacgt gggaaaaata agcagtctct tgatgctgtg gagaaagcag cgttctttgt
     2161 gacgttagat gaaactgaag aaggatacag aagtgaagac ccggatacgt caatggacag
     2221 ctacgccaaa tctctactac acggccgatg ttacgacagg tggtttgaca agtcgttcac
     2281 gtttgttgtc ttcaaaaacg ggaagatggg cctcaacgct gaacactcct gggcagatgc
     2341 gccgatcgtg gcccaccttt gggagtacgt catgtccatt gacagcctcc agctgggcta
     2401 tgcggaggat gggcactgca aaggcgacat caatccgaac attccgtacc ccaccaggct
     2461 gcagtgggac atcccggggg aatgtcaaga ggttatagag acctccctga acaccgcaaa
     2521 tcttctggca aacgacgtgg atttccattc cttcccattc gtagcctttg gtaaaggaat
     2581 catcaagaaa tgtcgcacga gcccagacgc ctttgtgcag ctggccctcc agctggcgca
     2641 ctacaaggac atgggcaagt tttgcctcac atacgaggcc tccatgaccc ggctcttccg
     2701 agaggggagg acggagaccg tgcgctcctg caccactgag tcatgcgact tcgtgcgggc
     2761 catggtggac ccggcccaga cggtggaaca gaggctgaag ttgttcaagt tggcgtctga
     2821 gaagcatcag catatgtatc gcctcgccat gaccggctct gggatcgatc gtcacctctt
     2881 ctgcctttac gtggtgtcta aatatctcgc tgtggagtcc cctttcctta aggaagtttt
     2941 atctgagcct tggagattat caacaagcca gacccctcag cagcaagtgg agctgtttga
     3001 cttggagaat aacccagagt acgtgtccag cggagggggc tttggaccgg ttgctgatga
     3061 cggctatggt gtgtcgtaca tccttgtggg agagaacctc atcaatttcc acatttcttc
     3121 caagttctct tgccctgaga cggattctca tcgctttgga aggcacctga aagaagcaat
     3181 gactgacatc atcactttgt ttggtctcag ttctaattcc aaaaagtaac tcgagtctag
     3241 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     3301 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     3361 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     3421 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     3481 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     3541 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3601 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3661 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3721 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     3781 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     3841 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     3901 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     3961 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     4021 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     4081 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4141 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4201 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     4261 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     4321 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     4381 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     4441 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     4501 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     4561 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     4621 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     4681 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     4741 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     4801 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     4861 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     4921 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     4981 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     5041 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     5101 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     5161 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     5221 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     5281 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     5341 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5401 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5461 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     5521 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     5581 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     5641 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     5701 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     5761 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5821 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5881 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5941 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6001 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6061 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6121 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6181 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6241 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6301 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6361 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6421 gctcttgatc cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga
     6481 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6541 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6601 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6661 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6721 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6781 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6841 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6901 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6961 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7021 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7081 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7141 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7201 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7261 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7321 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7381 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7441 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7501 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7561 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7621 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     7681 cgggagatct cccgatcccc tatggt
//
